Oscillating perfusion of cell suspensions through three-dimensional scaffolds enhances cell seeding efficiency and uniformity

2003 ◽  
Vol 84 (2) ◽  
pp. 205-214 ◽  
Author(s):  
D. Wendt ◽  
A. Marsano ◽  
M. Jakob ◽  
M. Heberer ◽  
I. Martin
Keyword(s):  
Three Dimensional ◽  
Cell Suspensions ◽  
Cell Seeding ◽  
Seeding Efficiency

scholarly journals The Influence of Scaffold Designs on Cell Seeding Efficiency in Establishing A Three-Dimensional Culture

2018 ◽  
Vol 1082 ◽  
pp. 012072
Author(s):  
Yanny Marliana Baba Ismail ◽  
Yvonne Reinwald ◽  
Ian Wimpenny ◽  
Oana Bretcanu ◽  
Kenneth Dalgarno ◽  
...  
Keyword(s):  
Three Dimensional ◽  
Cell Seeding ◽  
Three Dimensional Culture ◽  
Seeding Efficiency

scholarly journals The importance of factorial design in tissue engineering and biomaterials science: Optimisation of cell seeding efficiency on dermal scaffolds as a case study

2018 ◽  
Vol 9 ◽  
pp. 204173141878169 ◽  
Author(s):  
Alexandra Levin ◽  
Vaibhav Sharma ◽  
Lilian Hook ◽  
Elena García-Gareta
Keyword(s):  
Tissue Engineering ◽  
Experimental Design ◽  
Factorial Design ◽  
Three Dimensional ◽  
Cell Types ◽  
Cell Seeding ◽  
Factorial Experimental Design ◽  
Seeding Efficiency

This article presents a case study to show the usefulness and importance of using factorial design in tissue engineering and biomaterials science. We used a full factorial experimental design (2 × 2 × 2 × 3) to solve a routine query in every biomaterial research project: the optimisation of cell seeding efficiency for pre-clinical in vitro cell studies, the importance of which is often overlooked. In addition, tissue-engineered scaffolds can be cellularised with relevant cell type(s) to form implantable tissue constructs, where the cell seeding method must be reliable and robust. Our results show the complex relationship between cells and scaffolds and suggest that the optimum seeding conditions for each material may be different due to different material properties, and therefore, should be investigated for individual scaffolds. Our factorial experimental design can be easily translated to other cell types and three-dimensional biomaterials, where multiple interacting variables can be thoroughly investigated for better understanding of cell–biomaterial interactions.

scholarly journals Increased cell seeding efficiency in bioplotted three-dimensional PEOT/PBT scaffolds

2013 ◽  
Vol 10 (8) ◽  
pp. 679-689 ◽  
Author(s):  
A. M. Leferink ◽  
W. J. Hendrikson ◽  
J. Rouwkema ◽  
M. Karperien ◽  
C. A. van Blitterswijk ◽  
...  
Keyword(s):  
Three Dimensional ◽  
Cell Seeding ◽  
Seeding Efficiency

scholarly journals Three-dimensional plotted scaffolds with controlled pore size gradients: Effect of scaffold geometry on mechanical performance and cell seeding efficiency

2011 ◽  
Vol 7 (3) ◽  
pp. 1009-1018 ◽  
Author(s):  
Jorge M. Sobral ◽  
Sofia G. Caridade ◽  
Rui A. Sousa ◽  
João F. Mano ◽  
Rui L. Reis
Keyword(s):  
Pore Size ◽  
Mechanical Performance ◽  
Three Dimensional ◽  
Cell Seeding ◽  
Seeding Efficiency

Generation of three-dimensional pannus-like tissues in vitro from single cell suspensions of synovial fluid cells from arthritis patients

2004 ◽  
Vol 24 (2) ◽  
pp. 71-76 ◽  
Author(s):  
Samuel Solomon ◽  
Madhan Masilamani ◽  
Subhasis Mohanty ◽  
J�rg E. Schwab ◽  
Eva-Maria Boneberg ◽  
...  
Keyword(s):  
Synovial Fluid ◽  
Single Cell ◽  
Three Dimensional ◽  
Cell Suspensions ◽  
Synovial Fluid Cells

scholarly journals Electrodeposition of Hydroxyapatite on a Metallic 3D-Woven Bioscaffold

Coatings ◽  
2020 ◽  
Vol 10 (8) ◽  
pp. 715 ◽  
Author(s):  
Ju Xue ◽  
Ashley Farris ◽  
Yunfei Wang ◽  
Weiyan Yeh ◽  
Cristina Romany ◽  
...  
Keyword(s):  
Reference Electrode ◽  
Deposition Potential ◽  
Cellular Adhesion ◽  
Cell Seeding ◽  
X Ray Diffraction ◽  
X Ray ◽  
Woven Bone ◽  
Electrochemically Deposited ◽  
Seeding Efficiency ◽  
Stromal Stem Cells

In this study, we demonstrate that a uniform coating of hydroxyapatite (HAp, Ca10(PO4)6(OH)2) can be electrochemically deposited onto metallic 3D-woven bone scaffolds to enhance their bioactivity. The HAp coatings were deposited onto metallic scaffolds using an electrolyte containing Ca(NO3)2·4H2O, NH4H2PO4, and NaNO3. The deposition potential was varied to maximize the uniformity and adhesion of the coating. Using X-ray diffraction (XRD), Raman spectroscopy, and energy-dispersive spectroscopy (EDS), we found crystallized HAp on the 3D-woven lattice under all deposition potentials, while the −1.5 V mercury sulfate reference electrode potential provided the best local uniformity with a satisfactory deposition rate. The coatings generated under this optimized condition were approximately 5 µm thick and uniform throughout the internal and external sections of the woven lattice. We seeded and cultured both coated and uncoated scaffolds with human adipose-derived stromal/stem cells (ASCs) for 12 h and 4 days. We observed that the HAp coating increased the initial cell seeding efficiency by approximately 20%. Furthermore, after 4 days of culture, ASCs cultured on HAp-coated stainless-steel scaffolds increased by 32% compared to only 17% on the uncoated scaffold. Together, these results suggest that the HAp coating improves cellular adhesion.

scholarly journals Self-organising human gonads generated by a Matrigel-based gradient system

BMC Biology ◽  
2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Elizabeth Oliver ◽  
João Pedro Alves-Lopes ◽  
Femke Harteveld ◽  
Rod T. Mitchell ◽  
Elisabet Åkesson ◽  
...  
Keyword(s):  
Cell Differentiation ◽  
Germ Cell ◽  
Somatic Cell ◽  
Three Dimensional ◽  
Cell Suspensions ◽  
Testicular Development ◽  
Female Gonad ◽  
Gradient System ◽  
Three Dimensional Culture

Abstract Background Advances in three-dimensional culture technologies have led to progression in systems used to model the gonadal microenvironment in vitro. Despite demonstrating basic functionality, tissue organisation is often limited. We have previously detailed a three-dimensional culture model termed the three-layer gradient system to generate rat testicular organoids in vitro. Here we extend the model to human first-trimester embryonic gonadal tissue. Results Testicular cell suspensions reorganised into testis-like organoids with distinct seminiferous-like cords situated within an interstitial environment after 7 days. In contrast, tissue reorganisation failed to occur when mesonephros, which promotes testicular development in vivo, was included in the tissue digest. Organoids generated from dissociated female gonad cell suspensions formed loosely organised cords after 7 days. In addition to displaying testis-specific architecture, testis-like organoids demonstrated evidence of somatic cell differentiation. Within the 3-LGS, we observed the onset of AMH expression in the cytoplasm of SOX9-positive Sertoli cells within reorganised testicular cords. Leydig cell differentiation and onset of steroidogenic capacity was also revealed in the 3-LGS through the expression of key steroidogenic enzymes StAR and CYP17A1 within the interstitial compartment. While the 3-LGS generates a somatic cell environment capable of supporting germ cell survival in ovarian organoids germ cell loss was observed in testicular organoids. Conclusion The 3-LGS can be used to generate organised whole gonadal organoids within 7 days. The 3-LGS brings a new opportunity to explore gonadal organogenesis and contributes to the development of more complex in vitro models in the field of developmental and regenerative medicine.

scholarly journals A Perfusion Bioreactor System for Cell Seeding and Oxygen-Controlled Cultivation of Three-Dimensional Cell Cultures

2018 ◽  
Vol 24 (10) ◽  
pp. 585-595 ◽  
Author(s):  
Jakob Schmid ◽  
Sascha Schwarz ◽  
Robert Meier-Staude ◽  
Stefanie Sudhop ◽  
Hauke Clausen-Schaumann ◽  
...  
Keyword(s):  
Cell Cultures ◽  
Three Dimensional ◽  
Perfusion Bioreactor ◽  
Cell Seeding ◽  
Bioreactor System ◽  
Dimensional Cell

Polarized protein membrane for high cell seeding efficiency

2007 ◽  
Vol 83B (2) ◽  
pp. 472-480 ◽  
Author(s):  
Björn Atthoff ◽  
Cecilia Aulin ◽  
Catharina Adelöw ◽  
Jöns Hilborn
Keyword(s):  
Cell Seeding ◽  
High Cell ◽  
Protein Membrane ◽  
Seeding Efficiency
Sign in / Sign up

Export Citation Format

Share Document