MIRACLE: mass isotopomer ratio analysis of U-13C-labeled extracts. A new method for accurate quantification of changes in concentrations of intracellular metabolites

2004 ◽  
Vol 85 (6) ◽  
pp. 620-628 ◽  
Author(s):  
M. R. Mashego ◽  
L. Wu ◽  
J. C. Van Dam ◽  
C. Ras ◽  
J. L. Vinke ◽  
...  
2001 ◽  
Vol 88 (2) ◽  
pp. 385-386 ◽  
Author(s):  
John E. Barbuto

Previously published research is examined, and an alternative scoring method for the Motivation Sources Inventory is proposed. The new method, ratio analysis, provides an empirical assessment more consistent with the theoretical framework of the inventory. Theoretical and empirical support for ratio analysis is provided.


PLoS ONE ◽  
2014 ◽  
Vol 9 (11) ◽  
pp. e113909 ◽  
Author(s):  
Nikolas Kessler ◽  
Frederik Walter ◽  
Marcus Persicke ◽  
Stefan P. Albaum ◽  
Jörn Kalinowski ◽  
...  

2009 ◽  
Vol 76 (5) ◽  
pp. 1674-1678 ◽  
Author(s):  
Claude Saint-Ruf ◽  
Corinne Cordier ◽  
J�rome M�gret ◽  
Ivan Matic

ABSTRACT We have developed a new method for accurate quantification of dead microbial cells. This technique employs the simultaneous use of fluorescent hydrazides and nucleic acid dyes. Fluorescent hydrazides allow detection of cells that cannot be detected with currently used high-affinity nucleic acid dyes. This is particularly important for nongrowing bacterial populations and for multicellular communities containing physiologically heterogeneous cell populations, such as colonies and biofilms.


Author(s):  
C. C. Clawson ◽  
L. W. Anderson ◽  
R. A. Good

Investigations which require electron microscope examination of a few specific areas of non-homogeneous tissues make random sampling of small blocks an inefficient and unrewarding procedure. Therefore, several investigators have devised methods which allow obtaining sample blocks for electron microscopy from region of tissue previously identified by light microscopy of present here techniques which make possible: 1) sampling tissue for electron microscopy from selected areas previously identified by light microscopy of relatively large pieces of tissue; 2) dehydration and embedding large numbers of individually identified blocks while keeping each one separate; 3) a new method of maintaining specific orientation of blocks during embedding; 4) special light microscopic staining or fluorescent procedures and electron microscopy on immediately adjacent small areas of tissue.


1960 ◽  
Vol 23 ◽  
pp. 227-232 ◽  
Author(s):  
P WEST ◽  
G LYLES
Keyword(s):  

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