scholarly journals A cell engineering strategy to enhance supercoiled plasmid DNA production for gene therapy

2016 ◽  
Vol 113 (9) ◽  
pp. 2064-2071 ◽  
Author(s):  
Sally Hassan ◽  
Eli Keshavarz-Moore ◽  
John Ward
Keyword(s):  
Gene Therapy ◽  
Plasmid Dna ◽  
Cell Engineering ◽  
Supercoiled Plasmid ◽  
Dna Production ◽  
Engineering Strategy ◽  
A Cell

Host strain influences on supercoiled plasmid DNA production inEscherichia coli: Implications for efficient design of large-scale processes

2008 ◽  
Vol 101 (3) ◽  
pp. 529-544 ◽  
Author(s):  
Sin Yee Yau ◽  
Eli Keshavarz-Moore ◽  
John Ward
Keyword(s):  
Plasmid Dna ◽  
Large Scale ◽  
Host Strain ◽  
Inescherichia Coli ◽  
Efficient Design ◽  
Supercoiled Plasmid ◽  
Dna Production

Nanoparticle mediated delivery of pure P53 supercoiled plasmid DNA for gene therapy

2011 ◽  
Vol 156 (2) ◽  
pp. 212-222 ◽  
Author(s):  
Vítor M. Gaspar ◽  
Ilídio J. Correia ◽  
Ângela Sousa ◽  
Filomena Silva ◽  
Catarina M. Paquete ◽  
...  
Keyword(s):  
Gene Therapy ◽  
Plasmid Dna ◽  
Supercoiled Plasmid

scholarly journals A Cell Engineering Strategy to Enhance the Safety of Stem Cell Therapies

Cell Reports ◽  
2014 ◽  
Vol 8 (6) ◽  
pp. 1677-1685 ◽  
Author(s):  
Elisa Oricchio ◽  
Eirini P. Papapetrou ◽  
Fabien Lafaille ◽  
Yosif M. Ganat ◽  
Sonja Kriks ◽  
...  
Keyword(s):  
Stem Cell ◽  
Cell Engineering ◽  
Stem Cell Therapies ◽  
Cell Therapies ◽  
Engineering Strategy ◽  
A Cell

Polymeric Delivery of Proteins and Plasmid DNA for Tissue Engineering and Gene Therapy

2001 ◽  
Vol 11 (1-3) ◽  
pp. 12 ◽  
Author(s):  
Thomas P. Richardson ◽  
William L. Murphy ◽  
David J. Mooney
Keyword(s):  
Tissue Engineering ◽  
Gene Therapy ◽  
Plasmid Dna ◽  
Polymeric Delivery

Arf6-mediated macropinocytosis enhanced suicide gene therapy of C16TAB-condensed Tat/pDNA nanoparticles in ovarian cancer

Nanoscale ◽  
2021 ◽  
Author(s):  
Zhe Sun ◽  
Jinhai Huang ◽  
Linjia Su ◽  
Jing Li ◽  
Fangzheng Qi ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Gene Therapy ◽  
Cancer Treatment ◽  
Plasmid Dna ◽  
Suicide Gene ◽  
Cell Penetrating Peptides ◽  
Therapeutic Gene ◽  
Hiv Tat ◽  
Efficient Delivery ◽  
Cell Penetrating

Using cell-penetrating peptides (CPPs), typically HIV-Tat, to deliver the therapeutic gene for cancer treatment has being hampered by low efficient delivery and complicated uptake route of plasmid DNA (pDNA). On...

Emerging significance of plasmid DNA nuclear import in gene therapy

2003 ◽  
Vol 55 (6) ◽  
pp. 749-760 ◽  
Author(s):  
F Munkonge
Keyword(s):  
Gene Therapy ◽  
Plasmid Dna ◽  
Nuclear Import

scholarly journals Identification of a new deoxyendonuclease fromEscherichia colithat preferentially cleaves supercoiled plasmid DNA

FEBS Letters ◽  
1983 ◽  
Vol 153 (1) ◽  
pp. 60-64 ◽  
Author(s):  
Ujjala DasGupta ◽  
Sudhamoy Ghosh
Keyword(s):  
Plasmid Dna ◽  
Supercoiled Plasmid

scholarly journals Combination gene therapy for HIV using a conditional suicidal gene with CCR5 knockout

2021 ◽  
Vol 18 (1) ◽  
Author(s):  
Tugba Mehmetoglu-Gurbuz ◽  
Rose Yeh ◽  
Himanshu Garg ◽  
Anjali Joshi
Keyword(s):  
Gene Therapy ◽  
Lentiviral Vector ◽  
Suicide Gene ◽  
Vector System ◽  
Tat Protein ◽  
Ccr5 Gene ◽  
Hematopoietic Stem ◽  
Therapy Strategy ◽  
Hiv Tat ◽  
A Cell

Abstract Background Gene therapy approaches using hematopoietic stem cells to generate an HIV resistant immune system have been shown to be successful. The deletion of HIV co-receptor CCR5 remains a viable strategy although co-receptor switching to CXCR4 remains a major pitfall. To overcome this, we designed a dual gene therapy strategy that incorporates a conditional suicide gene and CCR5 knockout (KO) to overcome the limitations of CCR5 KO alone. Methods A two-vector system was designed that included an integrating lentiviral vector that expresses a HIV Tat dependent Thymidine Kinase mutant SR39 (TK-SR39) and GFP reporter gene. The second non-integrating lentiviral (NIL) vector expresses a CCR5gRNA-CRISPR/Cas9 cassette and HIV Tat protein. Results Transduction of cells sequentially with the integrating followed by the NIL vector allows for insertion of the conditional suicide gene, KO of CCR5 and transient expression of GFP to enrich the modified cells. We used this strategy to modify TZM cells and generate a cell line that was resistant to CCR5 tropic viruses while permitting infection of CXCR4 tropic viruses which could be controlled via treatment with Ganciclovir. Conclusions Our study demonstrates proof of principle that a combination gene therapy for HIV is a viable strategy and can overcome the limitation of editing CCR5 gene alone.

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