Hydrocyclones as cell retention device for CHO perfusion processes in single‐use bioreactors

2020 ◽  
Vol 117 (7) ◽  
pp. 1915-1928 ◽  
Author(s):  
Ioná W. Bettinardi ◽  
Andreas Castan ◽  
Ricardo A. Medronho ◽  
Leda R. Castilho
Keyword(s):  
Cell Retention ◽  
Single Use ◽  
Retention Device

scholarly journals Cell culture–based production of defective interfering influenza A virus particles in perfusion mode using an alternating tangential flow filtration system

2021 ◽  
Author(s):  
Marc D. Hein ◽  
Anshika Chawla ◽  
Maurizio Cattaneo ◽  
Sascha Y. Kupke ◽  
Yvonne Genzel ◽  
...  
Keyword(s):  
Influenza A Virus ◽  
Hollow Fiber ◽  
Influenza A ◽  
Tubular Cell ◽  
Cell Retention ◽  
Virus Particles ◽  
Tangential Flow ◽  
Perfusion Process ◽  
Flow Filtration ◽  
Retention Device

AbstractRespiratory diseases including influenza A virus (IAV) infections represent a major threat to human health. While the development of a vaccine requires a lot of time, a fast countermeasure could be the use of defective interfering particles (DIPs) for antiviral therapy. IAV DIPs are usually characterized by a large internal deletion in one viral RNA segment. Consequentially, DIPs can only propagate in presence of infectious standard viruses (STVs), compensating the missing gene function. Here, they interfere with and suppress the STV replication and might act “universally” against many IAV subtypes. We recently reported a production system for purely clonal DIPs utilizing genetically modified cells. In the present study, we established an automated perfusion process for production of a DIP, called DI244, using an alternating tangential flow filtration (ATF) system for cell retention. Viable cell concentrations and DIP titers more than 10 times higher than for a previously reported batch cultivation were observed. Furthermore, we investigated a novel tubular cell retention device for its potential for continuous virus harvesting into the permeate. Very comparable performances to typically used hollow fiber membranes were found during the cell growth phase. During the virus replication phase, the tubular membrane, in contrast to the hollow fiber membrane, allowed 100% of the produced virus particles to pass through. To our knowledge, this is the first time a continuous virus harvest was shown for a membrane-based perfusion process. Overall, the process established offers interesting possibilities for advanced process integration strategies for next-generation virus particle and virus vector manufacturing.Key points• An automated perfusion process for production of IAV DIPs was established.• DIP titers of 7.40E + 9 plaque forming units per mL were reached.• A novel tubular cell retention device enabled continuous virus harvesting.

scholarly journals Novel small scale TFF cell retention device for perfusion cell culture systems

2015 ◽  
Vol 9 (S9) ◽  
Author(s):  
Chris S Martin ◽  
Jorge Padilla-Zamudio ◽  
Doug Rank ◽  
Patrick McInnis ◽  
Mikhail Kozlov ◽  
...  
Keyword(s):  
Cell Culture ◽  
Small Scale ◽  
Cell Retention ◽  
Perfusion Cell Culture ◽  
Culture Systems ◽  
Cell Culture Systems ◽  
Retention Device

Continuous Cell Separation Using Microfluidic-Based Cell Retention Device with Alternative Boosted Flow

2020 ◽  
Vol 191 (1) ◽  
pp. 151-163
Author(s):  
Po-Hung Chen ◽  
Yu-Ting Cheng ◽  
Bing-Syuan Ni ◽  
Jen-Huang Huang
Keyword(s):  
Cell Separation ◽  
Cell Retention ◽  
Retention Device

scholarly journals Miniature auto‐perfusion bioreactor system with spiral microfluidic cell retention device

2021 ◽  
Author(s):  
Lu Yin ◽  
Wen Yip Au ◽  
Chia Chen Yu ◽  
Taehong Kwon ◽  
Zhangxing Lai ◽  
...  
Keyword(s):  
Perfusion Bioreactor ◽  
Cell Retention ◽  
Bioreactor System ◽  
Retention Device

scholarly journals Microfluidic Cell Retention Device for Perfusion of Mammalian Suspension Culture

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Taehong Kwon ◽  
Holly Prentice ◽  
Jonas De Oliveira ◽  
Nyasha Madziva ◽  
Majid Ebrahimi Warkiani ◽  
...  
Keyword(s):  
Suspension Culture ◽  
Cell Retention ◽  
Retention Device

Single-Use Views

2008 ◽  
Vol 33 (3) ◽  
pp. 139
Keyword(s):  
Single Use

DETERMINATION OF THE TYPE OF A SINGLE-USE GRENADE LAUNCHER BASED ON ITS COMPOSITE PARTS AND FRAGMENTS OF REACTIVE GRENADE FOUND AT THE PLACE OF ACCIDENT

2017 ◽  
Vol 17 ◽  
pp. 245-252
Author(s):  
V. V. Somov
Keyword(s):  
Combustion Chamber ◽  
Structural Features ◽  
Outlet Section ◽  
Technical Expertise ◽  
Jet Engine ◽  
Size Ofthe ◽  
Single Use ◽  
Nozzle Block ◽  
Considerable Damage

In carrying out an investigation into the explosion, among others, the investigative version of the use of a single-use reactive grenade launcher is being considered. The most common for criminal explosions are applied grenade launchers RPG-18, RPG-22, RPG-26. Their use is due to a number of such properties as small size and weight, which makes it possible to transfer them covertly, the range of the shot significantly exceeding the range of the hand grenade throw, the high detonating effect of the rocket grenade explosion. The single-use rocket launchers are generally of the same design. Their differences are in the features of the components construction and dimensional characteristics, which are given in the article. On the basis of expert practice, details ofgrenade launchers that remain at the site of the explosion and have the least damage are determined. These details are the objects of investigation of the explosion technical expertise. These objects include launchers of grenade launchers and rocket parts ofjet grenades. The design features of the launchers, their dimensional characteristics and marking symbols make it possible to determine their belonging to a specific type of jet grenade launchers. Missile parts of jet grenades differ in the form of the combustion chamber of the jet engine, nozzle, in the size ofthe outlet section of the nozzle, in the form and size of the stabilizerfeathers. To determine the belonging of the rocket part of the grenade to a specific type ofjet grenade launcher, it’s necessary to establish a set of structural features and dimensional characteristics. At considerable damage of the combustion chamber of the jet engine, as a rule, the nozzle block remains intact that allows to define diameter of critical section of a nozzle, and on it to establish type of the used single-use grenade launcher.

RESEARCH: Dry Heat Processing of Single-Use Respirators and Surgical Masks

2020 ◽  
Vol 54 (6) ◽  
pp. 410-416
Author(s):  
Joyce M. Hansen ◽  
Scott Weiss ◽  
Terra A. Kremer ◽  
Myrelis Aguilar ◽  
Gerald McDonnell
Keyword(s):  
Microbial Contamination ◽  
Healthcare Providers ◽  
High Volume ◽  
Airborne Particles ◽  
Heat Processing ◽  
Protective Equipment ◽  
Healthcare Facilities ◽  
Dry Heat ◽  
Surgical Masks ◽  
Single Use

The COVID-19 pandemic, caused by severe acute respiratory syndrome coronavirus 2, has challenged healthcare providers in maintaining the supply of critical personal protective equipment, including single-use respirators and surgical masks. Single-use respirators and surgical masks can reduce risks from the inhalation of airborne particles and microbial contamination. The recent high-volume demand for single-use respirators and surgical masks has resulted in many healthcare facilities considering processing to address critical shortages. The dry heat process of 80°C (176°F) for two hours (120 min) has been confirmed to be an appropriate method for single-use respirator and surgical mask processing.

Governments And Companies Move To Get Rid Of Single Use Plastics

2018 ◽  
Author(s):  
Daniel Nelson
Keyword(s):  
Single Use
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