scholarly journals Miniature auto‐perfusion bioreactor system with spiral microfluidic cell retention device

2021 ◽  
Author(s):  
Lu Yin ◽  
Wen Yip Au ◽  
Chia Chen Yu ◽  
Taehong Kwon ◽  
Zhangxing Lai ◽  
...  
Keyword(s):  
Perfusion Bioreactor ◽  
Cell Retention ◽  
Bioreactor System ◽  
Retention Device

Development of a Perfusion Bioreactor System for Alveolar Bone Tissue Engineering

2009 ◽  
Author(s):  
Ki Taek Lim ◽  
Pill Hoon Choung ◽  
Jang Ho Kim ◽  
Hyun Mok Son ◽  
Hoon Seonwoo ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Alveolar Bone ◽  
Perfusion Bioreactor ◽  
Bioreactor System

scholarly journals Human periosteal-derived cell expansion in a perfusion bioreactor system: proliferation, differentiation and extracellular matrix formation

2014 ◽  
Vol 11 (2) ◽  
pp. 519-530 ◽  
Author(s):  
M. Sonnaert ◽  
I. Papantoniou ◽  
V. Bloemen ◽  
G. Kerckhofs ◽  
F. P. Luyten ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Cell Expansion ◽  
Perfusion Bioreactor ◽  
Bioreactor System

scholarly journals Quantitative Validation of the Presto Blue™ Metabolic Assay for Online Monitoring of Cell Proliferation in a 3D Perfusion Bioreactor System

2015 ◽  
Vol 21 (6) ◽  
pp. 519-529 ◽  
Author(s):  
Maarten Sonnaert ◽  
Ioannis Papantoniou ◽  
Frank P. Luyten ◽  
Jan Schrooten
Keyword(s):  
Cell Proliferation ◽  
Online Monitoring ◽  
Perfusion Bioreactor ◽  
Quantitative Validation ◽  
Bioreactor System

Using 3D perfusion bioreactor system to studying cell biology of ovarian cancer.

2021 ◽  
Vol 39 (15_suppl) ◽  
pp. e17558-e17558
Author(s):  
Alba Martínez ◽  
Molly Buckley ◽  
Joel Berry ◽  
Rebecca Christian Arend
Keyword(s):  
Ovarian Cancer ◽  
Cell Culture ◽  
Cell Growth ◽  
Cell Lines ◽  
Culture Media ◽  
Tumor Biology ◽  
Perfusion Bioreactor ◽  
Suitable Model ◽  
Cell Culture Media ◽  
Bioreactor System

e17558 Background: Epithelial Ovarian Cancer (EOC) is the most common cause of death among gynecological malignancies. This is a result of the high rate of recurrence and chemo-resistance in EOC patients. Therefore, the development of new therapeutics is crucial. A major factor contributing to this is the lack of therapeutic candidates is lack of translational accuracy in preclinical models. Recently, 3-dimensional (3-D) models have aided in accurately recreating tumor biology. We have developed an EOC 3-D perfused bioreactor system that recapitulates EOC tumor biology and incorporates tumor biomechanical regulation. This model allows for us to more accurately predict the clinical response of new drug candidates, which aids in elimination of ineffective candidates prior to clinical trials. Methods: EOC cell lines (luciferase-taggedSKOV-3 and OVCAR-8) were embedded in a relevant extracellular matrix (ECM) and injected into a perfused, polydimethylsiloxane (PDMS) bioreactor. Microchannels were embedded in matrigel so that the cell culture media with or without chemotherapy could flow through the perfused PDMS to provide nutrient delivery and gas exchange enhancing viability and function of surrounding cells. The bioreactors were connected to a peristaltic pump that allowed for the cell culture media to perfuse over a 7-day period. We monitored cell viability using bioluminescence imaging (BLI), immunohistochemistry (IHC), and lactate dehydrogenase (LDH) release in media. Results: BLI showed a linear increase in SKOV-3 and OVCAR-8 cell growth over 7 days. These results were confirmed by IHC measuring the number of nucleated cells per micron2. Graphical representation of the region of interest (ROI) showed a high correlation between IHC staining of nucleated cells and BLI score. IHC analysis of PAX8 staining was positive and proved that the perfusion bioreactor system maintains EOC biology over time. In addition, our results suggest that the bioreactor is a suitable model for drug preclinical testing in both cell lines as well as in patients’ samples. Conclusions: Our preliminary results using the 3D EOC perfused, PDMS bioreactor model showed increased EOC cell growth overtime, while maintaining original EOC histology. Moreover, our results suggest that this model could provide a novel platform to study therapeutic interventions in EOC. Our ultimate goal is to implement ovarian cancer microenvironment components (e.g. immune cells) into bioreactor system to study different drug treatments to better determine drug candidate’s translational efficacy.

Hydrocyclones as cell retention device for CHO perfusion processes in single‐use bioreactors

2020 ◽  
Vol 117 (7) ◽  
pp. 1915-1928 ◽  
Author(s):  
Ioná W. Bettinardi ◽  
Andreas Castan ◽  
Ricardo A. Medronho ◽  
Leda R. Castilho
Keyword(s):  
Cell Retention ◽  
Single Use ◽  
Retention Device

Pulsatile Perfusion Bioreactor System for Durability Testing and Compliance Estimation of Tissue Engineered Vascular Grafts

2013 ◽  
Vol 41 (9) ◽  
pp. 1979-1989 ◽  
Author(s):  
Stefanos E. Diamantouros ◽  
Luis G. Hurtado-Aguilar ◽  
Thomas Schmitz-Rode ◽  
Petra Mela ◽  
Stefan Jockenhoevel
Keyword(s):  
Vascular Grafts ◽  
Perfusion Bioreactor ◽  
Durability Testing ◽  
Bioreactor System ◽  
Pulsatile Perfusion

scholarly journals A perfusion bioreactor system efficiently generates cell‐loaded bone substitute materials for addressing critical size bone defects

2015 ◽  
Vol 10 (11) ◽  
pp. 1727-1738 ◽  
Author(s):  
Claudia Kleinhans ◽  
Ramkumar Ramani Mohan ◽  
Gabriele Vacun ◽  
Thomas Schwarz ◽  
Barbara Haller ◽  
...  
Keyword(s):  
Bone Substitute ◽  
Critical Size ◽  
Bone Defects ◽  
Perfusion Bioreactor ◽  
Bone Substitute Materials ◽  
Bioreactor System ◽  
Substitute Materials

scholarly journals Cell culture–based production of defective interfering influenza A virus particles in perfusion mode using an alternating tangential flow filtration system

2021 ◽  
Author(s):  
Marc D. Hein ◽  
Anshika Chawla ◽  
Maurizio Cattaneo ◽  
Sascha Y. Kupke ◽  
Yvonne Genzel ◽  
...  
Keyword(s):  
Influenza A Virus ◽  
Hollow Fiber ◽  
Influenza A ◽  
Tubular Cell ◽  
Cell Retention ◽  
Virus Particles ◽  
Tangential Flow ◽  
Perfusion Process ◽  
Flow Filtration ◽  
Retention Device

AbstractRespiratory diseases including influenza A virus (IAV) infections represent a major threat to human health. While the development of a vaccine requires a lot of time, a fast countermeasure could be the use of defective interfering particles (DIPs) for antiviral therapy. IAV DIPs are usually characterized by a large internal deletion in one viral RNA segment. Consequentially, DIPs can only propagate in presence of infectious standard viruses (STVs), compensating the missing gene function. Here, they interfere with and suppress the STV replication and might act “universally” against many IAV subtypes. We recently reported a production system for purely clonal DIPs utilizing genetically modified cells. In the present study, we established an automated perfusion process for production of a DIP, called DI244, using an alternating tangential flow filtration (ATF) system for cell retention. Viable cell concentrations and DIP titers more than 10 times higher than for a previously reported batch cultivation were observed. Furthermore, we investigated a novel tubular cell retention device for its potential for continuous virus harvesting into the permeate. Very comparable performances to typically used hollow fiber membranes were found during the cell growth phase. During the virus replication phase, the tubular membrane, in contrast to the hollow fiber membrane, allowed 100% of the produced virus particles to pass through. To our knowledge, this is the first time a continuous virus harvest was shown for a membrane-based perfusion process. Overall, the process established offers interesting possibilities for advanced process integration strategies for next-generation virus particle and virus vector manufacturing.Key points• An automated perfusion process for production of IAV DIPs was established.• DIP titers of 7.40E + 9 plaque forming units per mL were reached.• A novel tubular cell retention device enabled continuous virus harvesting.

scholarly journals A Perfusion Bioreactor System for Cell Seeding and Oxygen-Controlled Cultivation of Three-Dimensional Cell Cultures

2018 ◽  
Vol 24 (10) ◽  
pp. 585-595 ◽  
Author(s):  
Jakob Schmid ◽  
Sascha Schwarz ◽  
Robert Meier-Staude ◽  
Stefanie Sudhop ◽  
Hauke Clausen-Schaumann ◽  
...  
Keyword(s):  
Cell Cultures ◽  
Three Dimensional ◽  
Perfusion Bioreactor ◽  
Cell Seeding ◽  
Bioreactor System ◽  
Dimensional Cell

scholarly journals Validation of a novel perfusion bioreactor system in cancer research

2020 ◽  
Vol 74 (3) ◽  
pp. 187-196
Author(s):  
Jasmina Stojkovska ◽  
Jovana Zvicer ◽  
Milena Milivojevic ◽  
Isidora Petrovic ◽  
Milena Stevanovic ◽  
...  
Keyword(s):  
Cancer Research ◽  
Drug Screening ◽  
Cancer Drug ◽  
Perfusion Bioreactor ◽  
Animal Testing ◽  
Anti Cancer ◽  
Medium Flow ◽  
Bioreactor System

Development of drugs is a complex, time- and cost-consuming process due to the lack of standardized and reliable characterization techniques and models. Traditionally, drug screening is based on in vitro analysis using two-dimensional (2D) cell cultures followed by in vivo animal testing. Unfortunately, application of the obtained results to humans in about 90 % of cases fails. Therefore, it is important to develop and improve cell-based systems that can mimic the in vivo-like conditions to provide more reliable results. In this paper, we present development and validation of a novel, user-friendly perfusion bioreactor system for single use aimed for cancer research, drug screening, anti-cancer drug response studies, biomaterial characterization, and tissue engineering. Simple design of the perfusion bioreactor provides direct medium flow at physiological velocities (100?250 ?m s-1) through samples of different sizes and shapes. Biocompatibility of the bioreactor was confirmed in short term cultivation studies of cervical carcinoma SiHa cells immobilized in alginate microfibers under continuous medium flow. The results have shown preserved cell viability indicating that the perfusion bioreactor in conjunction with alginate hydrogels as cell carriers could be potentially used as a tool for controlled anti-cancer drug screening in a 3D environment.

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