scholarly journals Vesicle transport and growth dynamics in Aspergillus niger : Microscale modeling of secretory vesicle flow and centerline extraction from confocal fluorescent data

2020 ◽  
Vol 117 (9) ◽  
pp. 2875-2886
Author(s):  
Philipp J. Kunz ◽  
Lars Barthel ◽  
Vera Meyer ◽  
Rudibert King
Keyword(s):  
Aspergillus Niger ◽  
Growth Dynamics ◽  
Secretory Vesicle ◽  
Vesicle Transport ◽  
Centerline Extraction ◽  
Microscale Modeling

Effects of Aspergillus niger Bio-Fertilizer on Growth Dynamics of Peanut Plants

2019 ◽  
Vol 09 (05) ◽  
pp. 337-343
Author(s):  
甜 张
Keyword(s):  
Aspergillus Niger ◽  
Growth Dynamics

scholarly journals Metabolism of phosphorus compoundsand taxonomic position of the Aspergillus niger AM1 mold

2020 ◽  
Vol 62 (6) ◽  
pp. 98-124
Author(s):  
Anton Z. Mindubaev ◽  
◽  
Elena K. Badeeva ◽  
Salima T. Minzanova ◽  
Lubov’ G. Mironova ◽  
...  
Keyword(s):  
Aspergillus Niger ◽  
Environmental Pollutants ◽  
Fungal Growth ◽  
Growth Dynamics ◽  
Sole Source ◽  
White Phosphorus ◽  
Phosphorus Compounds ◽  
Phosphoric Acid Ester ◽  
Relationship Of ◽  
First Time

White phosphorus is one of the most dangerous environmental pollutants. However, it is used in industry and for military purposes; therefore, it is impossible to overlook the fact that this substance is constantly released into the environment. In our works, cultures of microorganisms growing in media with a content of white phosphorus up to 1% were obtained for the first time. This exceeds the TLV in wastewater by 5000 times! These cultures are unique, and they are only in our possession. For the first time, cultures were grown in media containing white phosphorus as the sole source of phosphorus. In these environments, microorganisms grew without experiencing phosphorus starvation. That is, they oxidized white phosphorus to phosphate, which is necessary for vital activity! This is first ever example of the inclusion of white phosphorus in the biospheric circulation of the phosphorus element. It turned out that microorganisms that neutralize elemental phosphorus are able to biodegrade most of the spectrum of phosphorus compounds. Our studies of the metabolism of phosphorus-containing compounds of various classes confirm this. Since the chemistry of phosphorus is diverse, it is necessary to collect significant material on the metabolism of many classes of compounds. In this article, we describe the continuation of this work. It turned out that Aspergillus niger AM1 is able to utilize dithiophosphate of the simplest structure as sources of phosphorus, but is not able to utilize substituted dithiophosphonate. In addition, in the present work, we clarified the previously obtained results on the metabolism of phosphoric acid ester and phosphoramide.The NMR method demonstrated that A. niger AM1 slowly metabolizes hypophosphite resulting from the biodegradation of white phosphorus, but does not metabolize phosphite. The NMR data conforms to fungal growth dynamics with these substances in media. Also, was first studied phylogenetic relationship of A. niger AM1 with biodegradable A. niger and A. bombycis strains from the NCBI database.

scholarly journals Dinamika Populasi Jamur Pada Media Starter Tepung Beras Diperkaya Nutrisi Potato Dextrose Agar

2019 ◽  
Vol 5 (1) ◽  
pp. 1-6
Author(s):  
Anindyta Robiatul Adawiyah ◽  
Ahmad Syauqi ◽  
Hasan Zayadi
Keyword(s):  
Aspergillus Niger ◽  
Candida Utilis ◽  
Trichoderma Viride ◽  
Growth Dynamics ◽  
Rice Flour ◽  
Fungal Species ◽  
Potato Dextrose Agar ◽  
Test Method ◽  
Composite Flour ◽  
Fungal Cell

Yeast and fungus are important biomass in the food industry. Rice flour is one of the alternative basic ingredients of composite flour and consists of carbohydrates, fats, proteins, minerals and vitamins. Yeast multiplies by a process known as germination, which causes fermentation. This study aims to determine the development of fungal cell population dynamics in making starter with rice flour rich in PDA (Potato Dextrose Agar) nutrition. PDA (Potato Dextrose Agar) is one of the good media used to breed a microorganism, either in the form of fungus / function, bacteria, or living cells. The method used in this study is a different test method for two populations with 2 variables with PDA levels of 0% and 4% with 9 times of repetition. Which is observed once every eight hours the growth dynamics of the fungus by identifying the following fungal species Aspergillus niger is white and has a good composition, Candida utilis is white, Hansenulla anomala is opaque, Trichoderma viride is clear. Keywords: Rice flour, Yeast, PDA (Potato Dextrose Agar), Aspergillus niger, Candida utilis, Trichoderma viride, Hansenula saturnus. ABSTRAK Jamur adalah biomasa yang penting di dalam industri makanan. Tepung beras merupakan salah satu alternatif bahan dasar dari tepung komposit dan terdiri atas karbohidrat, lemak, protein, mineral dan vitamin. Starter adalah populasi mikroba dalam jumlah dan kondisi fisiologis yang siap diinokulasikan pada media fermentasi. Penelitian ini bertujuan Untuk mengetahui perkembangan dinamika populasi sel jamur pada pembuatan starter dengan media tepung beras kaya nutrisi PDA (Potato Dextrose Agar). PDA (Potato Dextrose Agar) merupakan salah satu media yang baik digunakan untuk membiakkan suatu mikroorganisme, baik itu berupa cendawan/fungsi, bakteri, maupun sel mahluk hidup. Metode yang digunakan pada penelitian ini adalah metode Uji beda rerata dua populasi dengan 2 variabel dengan kadar PDA 0% dan 4% dengan 9 kali ulangan waktu. Yang mana pada setiap 8 jam sekali diamati dinamika pertumbuhan jamurnya dengan mengidentifikasi spesies jamur sebagai berikut Aspergillus niger berwarna putih dan berbenang, Candida utilis berwarna putih, Hansenulla saturnus berwarna buram, Trichoderma viride berwarna bening. Kata Kunci : Tepung beras, Ragi, PDA (Potato Dextrose Agar), Aspergillus niger, Candida utilis, Trichoderma viride, Hansenula saturnus.

scholarly journals Yeast Rgd3 is a phospho-regulated F-BAR–containing RhoGAP involved in the regulation of Rho3 distribution and cell morphology

2020 ◽  
Vol 31 (23) ◽  
pp. 2570-2582
Author(s):  
Robert M. Gingras ◽  
Kyaw Myo Lwin ◽  
Abigail M. Miller ◽  
Anthony Bretscher
Keyword(s):  
Cell Polarity ◽  
Temperature Sensitivity ◽  
Cell Morphology ◽  
Budding Yeast ◽  
Secretory Vesicle ◽  
Vesicle Transport ◽  
Secretory Vesicles

RGD3 ( YHR182w) is a novel RhoGAP in budding yeast whose overexpression suppresses the temperature sensitivity of myo2 smy1 mutants defective in secretory vesicle transport. We show that Rgd3 localizes to polarized vesicles distinct from constitutive secretory vesicles and contributes to cell polarity through its GAP activity on Rho3.

scholarly journals Author response: Concerted action of kinesins KIF5B and KIF13B promotes efficient secretory vesicle transport to microtubule plus ends

2020 ◽  
Author(s):  
Andrea Serra-Marques ◽  
Maud Martin ◽  
Eugene A Katrukha ◽  
Ilya Grigoriev ◽  
Cathelijn AE Peeters ◽  
...  
Keyword(s):  
Secretory Vesicle ◽  
Vesicle Transport ◽  
Author Response ◽  
Concerted Action

scholarly journals Neuroprogenitor Cells From Patients With TBCK Encephalopathy Suggest Deregulation of Early Secretory Vesicle Transport

2022 ◽  
Vol 15 ◽  
Author(s):  
Danielle de Paula Moreira ◽  
Angela May Suzuki ◽  
André Luiz Teles e Silva ◽  
Elisa Varella-Branco ◽  
Maria Cecília Zorél Meneghetti ◽  
...  
Keyword(s):  
Cell Cycle Progression ◽  
Secretory Pathway ◽  
Molecular Mechanisms ◽  
Secretory Vesicle ◽  
Psychomotor Retardation ◽  
Vesicle Transport ◽  
Early Secretory Pathway ◽  
Pathogenic Variants ◽  
Altered Cell ◽  
Induced Pluripotent

Biallelic pathogenic variants in TBCK cause encephaloneuropathy, infantile hypotonia with psychomotor retardation, and characteristic facies 3 (IHPRF3). The molecular mechanisms underlying its neuronal phenotype are largely unexplored. In this study, we reported two sisters, who harbored biallelic variants in TBCK and met diagnostic criteria for IHPRF3. We provided evidence that TBCK may play an important role in the early secretory pathway in neuroprogenitor cells (iNPC) differentiated from induced pluripotent stem cells (iPSC). Lack of functional TBCK protein in iNPC is associated with impaired endoplasmic reticulum-to-Golgi vesicle transport and autophagosome biogenesis, as well as altered cell cycle progression and severe impairment in the capacity of migration. Alteration in these processes, which are crucial for neurogenesis, neuronal migration, and cytoarchitecture organization, may represent an important causative mechanism of both neurodevelopmental and neurodegenerative phenotypes observed in IHPRF3. Whether reduced mechanistic target of rapamycin (mTOR) signaling is secondary to impaired TBCK function over other secretory transport regulators still needs further investigation.

scholarly journals Concerted action of kinesins KIF5B and KIF13B promotes efficient secretory vesicle transport to microtubule plus ends

eLife ◽  
2020 ◽  
Vol 9 ◽  
Author(s):  
Andrea Serra-Marques ◽  
Maud Martin ◽  
Eugene A Katrukha ◽  
Ilya Grigoriev ◽  
Cathelijn AE Peeters ◽  
...  
Keyword(s):  
Golgi Apparatus ◽  
Intracellular Transport ◽  
Dominant Role ◽  
Secretory Vesicle ◽  
Vesicle Transport ◽  
Reverse Direction ◽  
Cell Periphery ◽  
Secretory Vesicles ◽  
Concerted Action ◽  
Directed Transport

Intracellular transport relies on multiple kinesins, but it is poorly understood which kinesins are present on particular cargos, what their contributions are and whether they act simultaneously on the same cargo. Here, we show that Rab6-positive secretory vesicles are transported from the Golgi apparatus to the cell periphery by kinesin-1 KIF5B and kinesin-3 KIF13B, which determine the location of secretion events. KIF5B plays a dominant role, whereas KIF13B helps Rab6 vesicles to reach freshly polymerized microtubule ends, to which KIF5B binds poorly, likely because its cofactors, MAP7-family proteins, are slow in populating these ends. Sub-pixel localization demonstrated that during microtubule plus-end directed transport, both kinesins localize to the vesicle front and can be engaged on the same vesicle. When vesicles reverse direction, KIF13B relocates to the middle of the vesicle, while KIF5B shifts to the back, suggesting that KIF5B but not KIF13B undergoes a tug-of-war with a minus-end directed motor.

scholarly journals Concerted action of kinesins KIF5B and KIF13B promotes efficient secretory vesicle transport to microtubule plus ends

2020 ◽  
Author(s):  
Andrea Serra-Marques ◽  
Maud Martin ◽  
Eugene A. Katrukha ◽  
Ilya Grigoriev ◽  
Cathelijn A.E. Peeters ◽  
...  
Keyword(s):  
Golgi Apparatus ◽  
Intracellular Transport ◽  
Dominant Role ◽  
Secretory Vesicle ◽  
Vesicle Transport ◽  
Reverse Direction ◽  
Cell Periphery ◽  
Secretory Vesicles ◽  
Concerted Action ◽  
Directed Transport

AbstractIntracellular transport relies on multiple kinesins, but it is poorly understood which kinesins are present on particular cargos, what their contributions are and whether they act simultaneously on the same cargo. Here, we show that Rab6-positive secretory vesicles are transported from the Golgi apparatus to the cell periphery by kinesin-1 KIF5B and kinesin-3 KIF13B, which determine the location of secretion events. KIF5B plays a dominant role, whereas KIF13B helps Rab6 vesicles to reach freshly polymerized microtubule ends, to which KIF5B binds poorly, likely because its cofactors, MAP7-family proteins, are slow in populating these ends. Sub-pixel localization demonstrated that during microtubule plus-end directed transport, both kinesins localize to the vesicle front and can be engaged on the same vesicle. When vesicles reverse direction, KIF13B relocates to the middle of the vesicle, while KIF5B shifts to the back, suggesting that KIF5B but not KIF13B undergoes a tug-of-war with a minus-end directed motor.

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