Determination of sialic acids in infant formula by liquid chromatography tandem mass spectrometry

2010 ◽  
Vol 24 (11) ◽  
pp. 1208-1212 ◽  
Author(s):  
L. K. Sørensen
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Tandem Mass Spectrometry ◽  
Infant Formula ◽  
Sialic Acids ◽  
Tandem Mass ◽  
Chromatography Tandem Mass Spectrometry ◽  
Liquid Chromatography Tandem Mass

scholarly journals Determination of Free and Total Choline and Free and Total Carnitine in Infant Formula and Adult/Pediatric Nutritional Formula by Liquid Chromatography/Tandem Mass Spectrometry (HPLC-MS/MS): A Multi-laboratory Testing Study, Final Action 2015.10

2020 ◽  
Vol 103 (6) ◽  
pp. 1560-1567
Author(s):  
Jeffrey Shippar ◽  
David Ellingson ◽  
Jane Sabbatini
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Tandem Mass Spectrometry ◽  
Infant Formula ◽  
Performance Testing ◽  
Tandem Mass ◽  
Chromatography Tandem Mass Spectrometry ◽  
Liquid Chromatography Tandem Mass ◽  
Total Carnitine

Abstract Background A multi-laboratory study was conducted on AOAC First Action Method 2015.10 “Determination of Free and Total Choline and Free and Total Carnitine in Infant Formula and Adult/Pediatric Nutritional Formula by Liquid Chromatography/Tandem Mass Spectrometry (HPLC-MS/MS).” Objective In this study, nine laboratories participated in the performance testing of the method using ten nutritional products tested as blind duplicates. Method Both free and total carnitine and free and total choline content of the samples were determined using separate extractions for the free and total results. For free choline and carnitine analysis, samples are diluted in water. For total choline and carnitine analysis, samples are extracted using acid-assisted microwave hydrolysis with nitric acid. For both the free and total methods, samples are then diluted with acetonitrile and analyzed using strong cation exchange (SCX) liquid chromatography coupled to a triple quadrupole tandem mass spectrometer (LCMS). Stable isotope labeled internal standards were utilized in all analyses to compensate for extraction inefficiencies and ionization suppression.

scholarly journals Determination of sialic acid in dietary supplement by liquid chromatography tandem-mass spectrometry (LC-MS/MS)

2019 ◽  
Vol 2 (3) ◽  
pp. 3-9
Author(s):  
Hong Ngoc Nguyen Thi ◽  
Thanh Hoa Mac Thi ◽  
Mai Hoa Duong Thi ◽  
Son Tran Hung ◽  
Khanh Cao Cong ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Tandem Mass Spectrometry ◽  
Dietary Supplement ◽  
Sialic Acids ◽  
Neuraminic Acid ◽  
Tandem Mass ◽  
Chromatography Tandem Mass Spectrometry ◽  
Liquid Chromatography Tandem Mass

Simultaneous determination of sialic acids (N-acetyl neuraminic acid - NANA and N-glycolyl neuraminic acid - NGNA) in dietary supplement matrices by liquid chromatography tandem-mass spectrometry (LC-MS/MS) had been developed. Sialic acids were separated by using a C18 column (75 mm x 3.9 mm, 2.5 µm) and isocratic mobile phase of acid formic 0.1 %: acetonitrile (80:20, v/v). Sialic acids were totally hydrolyzed by formic acid 1M at 80oC in 2 hours before being analyzed by LC-MS/MS. It was proved that the method with the linearity range of 0.04 - 4.25 µg/ml had highly specificity, selectivity, repeatability, and recovery rate, that well satisfied AOAC requirements. The method has been applied to detect sialic acids in commercial dietary supplements.

Sign in / Sign up

Export Citation Format

Share Document