Screening and identification of potential bioactive components in a combined prescription of Danggui Buxue decoction using cell extraction coupled with high performance liquid chromatography

2008 ◽  
Vol 22 (2) ◽  
pp. 157-163 ◽  
Author(s):  
Xi Zhang ◽  
Lian-Wen Qi ◽  
Ling Yi ◽  
Ping Li ◽  
Xiao-Dong Wen ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Bioactive Components ◽  
Cell Extraction ◽  
Screening And Identification ◽  
Danggui Buxue Decoction

Screening and Analyzing the Potential Bioactive Components from Reduning Injection, Using Macrophage Cell Extraction and Ultra-High Performance Liquid Chromatography Coupled with Mass Spectrometry

2013 ◽  
Vol 41 (01) ◽  
pp. 221-229 ◽  
Author(s):  
Yonghui Li ◽  
Peixiang Wang ◽  
Wei Xiao ◽  
Li Zhao ◽  
Zhenzhong Wang ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Cell Damage ◽  
Prostaglandin E ◽  
Bioactive Components ◽  
Macrophage Cell ◽  
Cell Extraction ◽  
Dicaffeoylquinic Acid ◽  
Reduning Injection

In this paper, a useful method for screening and analyzing the potential bioactive components from Reduning Injection was developed using macrophage cell extraction and ultra-high performance liquid chromatography coupled with Q-TOF/MS spectrometry. In addition, the protective effects on macrophage cell damage induced by LPS in vitro were also investigated. The results showed that chlorogenic acid, 3, 4-dicaffeoylquinic acid, 3, 5-dicaffeoylquinic acid and 4, 5-dicaffeoylquinic acid significantly inhibited the prostaglandin E2 (PGE2) release and reversed the interleukin-6 (IL-6) secretion of macrophages (p < 0.05). These data indicated that the method of macrophage cell extraction coupled with UPLC-MS technology is feasible, rapid and useful for screening and analyzing potential bioactive components from TCM injection.

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