Identification of a novel miRNA that increases transient protein expression in combination with valproic acid

2017 ◽  
Vol 33 (4) ◽  
pp. 1139-1145 ◽  
Author(s):  
Hermann-Josef Meyer ◽  
Dorothea Reilly ◽  
Scott E. Martin ◽  
Athena W. Wong
Keyword(s):  
Valproic Acid ◽  
Protein Expression ◽  
Transient Protein Expression ◽  
Novel Mirna

scholarly journals Automation of large scale transient protein expression in mammalian cells

2011 ◽  
Vol 175 (2) ◽  
pp. 209-215 ◽  
Author(s):  
Yuguang Zhao ◽  
Benjamin Bishop ◽  
Jordan E. Clay ◽  
Weixian Lu ◽  
Margaret Jones ◽  
...  
Keyword(s):  
Protein Expression ◽  
Mammalian Cells ◽  
Large Scale ◽  
Transient Protein Expression ◽  
Expression In Mammalian Cells

Optimization and Comparison of Different DNA Methyl Transferase and Histone Deacetylase Inhibitors for Enhancing Transient Protein Expression

2010 ◽  
pp. 261-264
Author(s):  
Gaurav Backliwal ◽  
Markus Hildinger ◽  
Ivan Küttel ◽  
David L. Hacker ◽  
Florian M. Wurm
Keyword(s):  
Protein Expression ◽  
Histone Deacetylase ◽  
Histone Deacetylase Inhibitors ◽  
Methyl Transferase ◽  
Transient Protein Expression

scholarly journals An anti-apoptotic HEK293 cell line provides a robust and high titer platform for transient protein expression in bioreactors

mAbs ◽  
2019 ◽  
Vol 11 (5) ◽  
pp. 977-986 ◽  
Author(s):  
Tia A Arena ◽  
Bernice Chou ◽  
Peter D. Harms ◽  
Athena W. Wong
Keyword(s):  
Protein Expression ◽  
Cell Line ◽  
Hek293 Cell ◽  
High Titer ◽  
Hek293 Cell Line ◽  
Transient Protein Expression

scholarly journals Agroinfiltration-based efficient transient protein expression in leguminous plants

2019 ◽  
Vol 36 (2) ◽  
pp. 119-123 ◽  
Author(s):  
Takuya Suzaki ◽  
Mai Tsuda ◽  
Hiroshi Ezura ◽  
Brad Day ◽  
Kenji Miura
Keyword(s):  
Protein Expression ◽  
Leguminous Plants ◽  
Transient Protein Expression

The impact ofPseudomonas syringaetype III effectors on transient protein expression in tobacco

Plant Biology ◽  
2014 ◽  
Vol 17 (2) ◽  
pp. 484-492 ◽  
Author(s):  
J. F. Buyel ◽  
J. J. Buyel ◽  
C. Haase ◽  
R. Fischer
Keyword(s):  
Protein Expression ◽  
Transient Protein Expression ◽  
The Impact

High throughput screening identifies novel, cell cycle-arresting small molecule enhancers of transient protein expression

2017 ◽  
Vol 33 (6) ◽  
pp. 1579-1588 ◽  
Author(s):  
Hermann-Josef Meyer ◽  
Rebecca Turincio ◽  
Shirley Ng ◽  
Juan Li ◽  
Blair Wilson ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Protein Expression ◽  
Small Molecule ◽  
High Throughput ◽  
High Throughput Screening ◽  
Transient Protein Expression ◽  
Small Molecule Enhancers

scholarly journals Enhancement of transient erythropoietin protein expression by valproic acid in CHO‐K1 suspension adapted cells

2020 ◽  
Vol 25 (1) ◽  
pp. 28
Author(s):  
Yana Rubiyana ◽  
Retno Damajanti Soejoedono ◽  
Adi Santoso
Keyword(s):  
Gene Expression ◽  
Valproic Acid ◽  
Protein Expression ◽  
Histone Deacetylase Inhibitors ◽  
Expression System ◽  
Transient Gene Expression ◽  
Optimum Concentration ◽  
Enzyme Linked Immunosorbent Assay ◽  
Stable Gene ◽  
Gene Expression System

Erythropoietin (EPO) is a therapeutic protein that is widely used to increase red blood cell production in chronic kidney failure. EPO protein can be produced quickly with a transient gene expression system (TGE). However, the titer produced using TGE is usually lower than the stable gene expression system (SGE). It has been known that TGE can be improved by histone deacetylase inhibitors (iHDACs) such as valproic acid (VPA). This study was conducted to examine the VPA effect on EPO protein expression in CHO‐K1 suspension adapted cells and to find the optimum concentration of VPA on transient EPO protein production. EPO proteins was quantified using the enzyme‐linked immunosorbent assay (ELISA) method. The optimization of VPA concentrations showed that VPA increased the EPO protein yield by up to 2‐fold in transient EPO production, and the optimum concentration of VPA was 4 mM. VPA optimization was very helpful to obtain the maximum increase in the transiently expressed protein. Furthermore, this study can be used as a model to produce EPO proteins or other recombinant proteins rapidly with TGE of CHO‐K1 suspension adapted cells.

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