pH-stat fed-batch process to enhance the production of cis, cis-muconate from benzoate by Pseudomonas putida KT2440-JD1

2011 ◽  
Vol 28 (1) ◽  
pp. 85-92 ◽  
Author(s):  
Jozef B. J. H. van Duuren ◽  
Dorien Wijte ◽  
Bianka Karge ◽  
Vítor A.P. Martins dos Santos ◽  
Yu Yang ◽  
...  
Keyword(s):  
Pseudomonas Putida ◽  
Batch Process ◽  
Fed Batch ◽  
Pseudomonas Putida Kt2440 ◽  
Ph Stat

scholarly journals Engineering of Solvent-Tolerant Pseudomonas putida S12 for Bioproduction of Phenol from Glucose

2005 ◽  
Vol 71 (12) ◽  
pp. 8221-8227 ◽  
Author(s):  
Nick J. P. Wierckx ◽  
Hendrik Ballerstedt ◽  
Jan A. M. de Bont ◽  
Jan Wery
Keyword(s):  
Pseudomonas Putida ◽  
High Throughput Screening ◽  
Random Mutagenesis ◽  
Batch Process ◽  
Shake Flask Culture ◽  
Efficient Production ◽  
Fed Batch ◽  
Tyrosine Phenol Lyase ◽  
Solvent Tolerant ◽  
Biphasic Medium

ABSTRACT Efficient bioconversion of glucose to phenol via the central metabolite tyrosine was achieved in the solvent-tolerant strain Pseudomonas putida S12. The tpl gene from Pantoea agglomerans, encoding tyrosine phenol lyase, was introduced into P. putida S12 to enable phenol production. Tyrosine availability was a bottleneck for efficient production. The production host was optimized by overexpressing the aroF-1 gene, which codes for the first enzyme in the tyrosine biosynthetic pathway, and by random mutagenesis procedures involving selection with the toxic antimetabolites m-fluoro-dl-phenylalanine and m-fluoro-l-tyrosine. High-throughput screening of analogue-resistant mutants obtained in this way yielded a P. putida S12 derivative capable of producing 1.5 mM phenol in a shake flask culture with a yield of 6.7% (mol/mol). In a fed-batch process, the productivity was limited by accumulation of 5 mM phenol in the medium. This toxicity was overcome by use of octanol as an extractant for phenol in a biphasic medium-octanol system. This approach resulted in accumulation of 58 mM phenol in the octanol phase, and there was a twofold increase in the overall production compared to a single-phase fed batch.

Production of Rhamnolipid Biosurfactant from Fed Batch Culture by Pseudomonas aeruginosa using Multiple Substrates

2020 ◽  
Vol 16 (6) ◽  
pp. 928-933
Author(s):  
Jujjavarapu S. Eswari
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Batch Process ◽  
Time Interval ◽  
Fed Batch ◽  
Multiple Substrates ◽  
Surface Active Agents ◽  
Limiting Nutrients ◽  
Limiting Nutrient ◽  
Surface Active ◽  
Rhamnolipid Biosurfactant

Objective: Biosurfactants are the surface active agents which are used for the reduction of surface and interfacial tensions of liquids. Rhamnolipids are the surfactants produced by Pseudomonas aeruginosa. It requires minimum nutrition for its growth as it can also grow in distilled water. The rhamnolipids produced by Pseudomonas aeruginosa are extra-cellular glycolipids consisting of L-rhamnose and 3-hydroxyalkanoic acid. Methods: The fed-batch method for the rhamnolipid production is considered in this study to know the influence of the carbon, nitrogen, phosphorous substrates as growth-limiting nutrients. Pulse feeding is employed for limiting nutrient addition at particular time interval to obtain maximum rhamnolipid formation from Pseudomonas aeruginosa compared with the batch process. Results: Out of 3 fed batch strategies constant glucose fed batch strategy shows best and gave maximum rhamnolipid concentration of 0.134 g/l.

scholarly journals Establishing a Fed‐Batch Process for Protease Expression with Bacillus licheniformis in Polymer‐Based Controlled‐Release Microtiter Plates

2019 ◽  
Vol 15 (2) ◽  
pp. 1900088 ◽  
Author(s):  
Tobias Habicher ◽  
Edward K. A. Rauls ◽  
Franziska Egidi ◽  
Timm Keil ◽  
Tobias Klein ◽  
...  
Keyword(s):  
Controlled Release ◽  
Bacillus Licheniformis ◽  
Batch Process ◽  
Fed Batch ◽  
Microtiter Plates ◽  
Protease Expression
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