Alterations in biochemical components of extracellular matrix in intervertebral disc herniation: role of MMP-2 and TIMP-2 in type II collagen loss

2006 ◽  
Vol 24 (5) ◽  
pp. 431-436 ◽  
Author(s):  
Leyla Didem Kozacı ◽  
Alev Guner ◽  
Gulgun Oktay ◽  
Gul Guner
Keyword(s):  
Extracellular Matrix ◽  
Intervertebral Disc ◽  
Disc Herniation ◽  
Type Ii Collagen ◽  
Intervertebral Disc Herniation ◽  
Type Ii ◽  
Biochemical Components

The Effect of Adenosine on Extracellular Matrix Production in Porcine Intervertebral Disc Cells

2018 ◽  
Vol 206 (1-2) ◽  
pp. 73-81 ◽  
Author(s):  
Xue Yin ◽  
Silvia  Gonzales ◽  
Somya Sha ◽  
Howard Levene ◽  
Chun-Yuh Huang
Keyword(s):  
Gene Expression ◽  
Extracellular Matrix ◽  
Intervertebral Disc ◽  
Adenosine Receptors ◽  
Type Ii Collagen ◽  
Underlying Mechanism ◽  
Receptor Activation ◽  
Type Ii ◽  
Atp Production ◽  
Intracellular Atp

Compressive loading promotes adenosine triphosphate (ATP) production and release by intervertebral disc (IVD) cells. Extracellular ATP can be rapidly hydrolyzed by ectonucleotidases. Adenosine, one of the adenine derivatives of ATP hydrolysis, can modulate diverse cellular actions via adenosine receptors. The objectives of this study were to investigate the effects of exogenous adenosine on the production of extracellular matrix (ECM; i.e., collagen type II and aggrecan) and ATP of IVD cells and explore the underlying mechanism of action. It was found that adenosine treatment significantly upregulated aggrecan and type II collagen gene expression and the ATP level in IVD cells. Dipyridamole, an adenosine transport blocker, completely suppressed the effects of adenosine on the ATP production and ECM gene expression of the IVD cells, whereas antagonists of adenosine receptors did not significantly affect adenosine-treated IVD cells. The findings suggested that elevated intracellular ATP and upregulation of ECM gene expression by adenosine treatment are mainly due to adenosine uptake rather than receptor activation. Since ECM biosynthesis is a high ATP demanding process, supplementing adenosine could be beneficial as IVD cells are able to utilize it to replenish intracellular ATP and sequentially promote ECM production, which is constantly suppressed by limited nutrition supply due to the avascular nature of the IVD.

scholarly journals On the role of type IX collagen in the extracellular matrix of cartilage: type IX collagen is localized to intersections of collagen fibrils.

1986 ◽  
Vol 102 (5) ◽  
pp. 1931-1939 ◽  
Author(s):  
W Müller-Glauser ◽  
B Humbel ◽  
M Glatt ◽  
P Sträuli ◽  
K H Winterhalter ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Bone Formation ◽  
Tissue Distribution ◽  
Chicken Embryo ◽  
Polyclonal Antibodies ◽  
Type Ii Collagen ◽  
Endochondral Bone Formation ◽  
Type Ii ◽  
Endochondral Bone

The tissue distribution of type II and type IX collagen in 17-d-old chicken embryo was studied by immunofluorescence using polyclonal antibodies against type II collagen and a peptic fragment of type IX collagen (HMW), respectively. Both proteins were found only in cartilage where they were co-distributed. They occurred uniformly throughout the extracellular matrix, i.e., without distinction between pericellular, territorial, and interterritorial matrices. Tissues that undergo endochondral bone formation contained type IX collagen, whereas periosteal and membranous bones were negative. The thin collagenous fibrils in cartilage consisted of type II collagen as determined by immunoelectron microscopy. Type IX collagen was associated with the fibrils but essentially was restricted to intersections of the fibrils. These observations suggested that type IX collagen contributes to the stabilization of the network of thin fibers of the extracellular matrix of cartilage by interactions of its triple helical domains with several fibrils at or close to their intersections.

Langzeitevaluation postoperativer Schmerzen nach thorakolumbaler Hemilaminektomie

2020 ◽  
Vol 48 (05) ◽  
pp. 377-377
Keyword(s):  
Postoperative Pain ◽  
Intervertebral Disc ◽  
Disc Herniation ◽  
Neuropathischer Schmerz ◽  
Intervertebral Disc Herniation ◽  
Pain Evaluation ◽  
Ca 50

Zidan N, Medland J, Olby NJ. Long-term postoperative pain evaluation in dogs with thoracolumbar intervertebral disc herniation after hemilaminectomy. J Vet Intern Med 2020. doi: 10.1111/jvim.15800. Beim Menschen ist chronischer neuropathischer Schmerz eine häufige Komplikation nach Rückenmarksinsulten, ca. 50–80 % der Patienten entwickeln eine derartige Dolenz. Für den Hund liegen diesbezüglich keine Studiendaten vor, vor allem aufgrund der herausfordernden Evaluierung insbesondere von neuropathischen Schmerzzuständen. Diese Studie prüfte die Verlässlichkeit einer Messung von spinalen mechanisch ausgelösten sensorischen Schwellenwerten (mechanical sensory threshholds, MSTs) und verglich die MSTs gesunder Hunde mit denen von Hunden nach operativer Hemilaminektomie aufgrund einer thorakolumbaren intervertebralen Diskherniation (IVDH).

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