scholarly journals Monitoring Dicer‐Mediated miRNA‐21 Maturation and Ago2 Loading by a Dual‐Colour FIT PNA Probe Set

ChemBioChem ◽  
2020 ◽  
Vol 21 (17) ◽  
pp. 2527-2532
Author(s):  
Natalia Loibl ◽  
Christoph Arenz ◽  
Oliver Seitz
Keyword(s):  
Author(s):  
Paolo Giamundo

Background: Minimally-invasive treatments for hemorrhoids should be encouraged as they cause low morbidity, reasonable discomfort and quicker return to work. According to the “vascular theory” hemorrhoidal disease is mainly caused by blood overflow into hemorrhoidal plexus deriving from the superior hemorrhoidal arteries. Introduction: Many different procedures have been described in the literature with the common goal of reducing the blood flow into the hemorrhoidal piles. ‘HeLP’ (Hemorrhoids Laser Procedure) is a novel form of dearterialization to treat patients suffering from symptomatic hemorrhoids. Methods: The procedure consists of the closure of the terminal branches of the superior rectal artery approximately 2-3 cm above the dentate line by means of laser shots originated by a diode laser platform. The arteries, at that level, have variable location and distribution. Therefore, a doppler probe set at the frequency of 20MHz helps identifying the arteries that would be missed otherwise. The laser beam is well tolerated by patients. For this reason, anesthesia is not required in most cases and the procedure allows a quick return to daily activities. In case of concomitant severe mucosal prolapse, the laser treatment can be combined with suture mucopexy. Three to six running sutures allow a complete lifting of hemorrhoidal piles, securing long-term resolution of symptoms. Results and Conclusions: ‘HeLP’ is indicated in patients with symptomatic hemorrhoids where conservative treatment failed and when mucosal prolapse is scarce or not symptomatic. The addition of mucopexy to laser treatment (HeLPexx) contributes to overall resolution of symptoms when mucosal prolapse is an issue, Emborrhoid is another novel, ‘hi-tech’ form of selective dearterialization used in selected case of hemorrhoids where main symptom is bleeding. It is generally used in cases where surgery is contraindicated due to severe concomitant diseases.


1983 ◽  
Vol 12 (1) ◽  
pp. 24-27
Author(s):  
K. Balasubramonian ◽  
N. Nithiyanandam ◽  
K. P. Rajappan
Keyword(s):  

2021 ◽  
Vol 9 (1) ◽  
Author(s):  
Jesse W. Breinholt ◽  
Sarah B. Carey ◽  
George P. Tiley ◽  
E. Christine Davis ◽  
Lorena Endara ◽  
...  

Science News ◽  
1969 ◽  
Vol 95 (17) ◽  
pp. 411 ◽  
Author(s):  
Ann Ewing
Keyword(s):  

Kerntechnik ◽  
2021 ◽  
Vol 86 (3) ◽  
pp. 236-243
Author(s):  
G. R. Sunaryo ◽  
R. Kusumastuti ◽  
Sriyono

Abstract The objective of this research is to understand the condition of the structural material of the 30 MW RSG-GAS research reactor as input for the aging management program. Furthermore, this should enable a prediction of the remaining life of the components. In the current experiment, corrosion surveillance was carried out at Interim Storage for Spent Fuel (ISSF), that has similar water quality as in reactor pool by using a corrosion probe which is made of aluminum alloy and stainless steel. The probe set is designed to understand the effect of water quality in the ISSF pond. The corrosion processes observed were pitting, crevice and galvanic corrosion. Two sets of corrosion probes were immersed into the ISSF pool in 2007, hanging by steel wire, 1-meter height from the bottom surface. One probe set consists of horizontal and vertical positions. The soaking time was 7 years. The observations made were water chemical content, corrosion rate and visual analysis, macro and micro. For macro visual observations an optical microscope was used, for micro-observations SEM-EDX. From the results of macro-observations, information on the presence of galvanic corrosion, crevice and pitting was obtained. SEM-EDX provides information on the influence of chloride ions on corrosion products. This experience will be very useful in dealing with the aging process of Indonesia’s nuclear power plants in the future.


Author(s):  
Noor D. White ◽  
Zachary A. Batz ◽  
Edward L. Braun ◽  
Michael J. Braun ◽  
Karen L. Carleton ◽  
...  
Keyword(s):  

2018 ◽  
Vol 68 (4) ◽  
pp. 594-606 ◽  
Author(s):  
Matthew G Johnson ◽  
Lisa Pokorny ◽  
Steven Dodsworth ◽  
Laura R Botigué ◽  
Robyn S Cowan ◽  
...  

Author(s):  
Harsha Doddapaneni ◽  
Sara Javornik Cregeen ◽  
Richard Sucgang ◽  
Qingchang Meng ◽  
Xiang Qin ◽  
...  

AbstractThe newly emerged and rapidly spreading SARS-CoV-2 causes coronavirus disease 2019 (COVID-19). To facilitate a deeper understanding of the viral biology we developed a capture sequencing methodology to generate SARS-CoV-2 genomic and transcriptome sequences from infected patients. We utilized an oligonucleotide probe-set representing the full-length genome to obtain both genomic and transcriptome (subgenomic open reading frames [ORFs]) sequences from 45 SARS-CoV-2 clinical samples with varying viral titers. For samples with higher viral loads (cycle threshold value under 33, based on the CDC qPCR assay) complete genomes were generated. Analysis of junction reads revealed regions of differential transcriptional activity and provided evidence of expression of ORF10. Heterogeneous allelic frequencies along the 20kb ORF1ab gene suggested the presence of a defective interfering viral RNA species subpopulation in one sample. The associated workflow is straightforward, and hybridization-based capture offers an effective and scalable approach for sequencing SARS-CoV-2 from patient samples.


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