Weather Probe Set near Barbados

Science News ◽  
1969 ◽  
Vol 95 (17) ◽  
pp. 411 ◽  
Author(s):  
Ann Ewing
Keyword(s):  
Probe Set

Hemorrhoidal Laser procedure (HeLP) and Hemorrrrhoidal Laser procedure + mucopexy (HeLPexx) and other Emerging Technologies

2020 ◽  
Vol 15 ◽  
Author(s):  
Paolo Giamundo
Keyword(s):  
Laser Treatment ◽  
Dentate Line ◽  
Mucosal Prolapse ◽  
Daily Activities ◽  
Common Goal ◽  
Superior Rectal Artery ◽  
Doppler Probe ◽  
The Common ◽  
Probe Set

Background: Minimally-invasive treatments for hemorrhoids should be encouraged as they cause low morbidity, reasonable discomfort and quicker return to work. According to the “vascular theory” hemorrhoidal disease is mainly caused by blood overflow into hemorrhoidal plexus deriving from the superior hemorrhoidal arteries. Introduction: Many different procedures have been described in the literature with the common goal of reducing the blood flow into the hemorrhoidal piles. ‘HeLP’ (Hemorrhoids Laser Procedure) is a novel form of dearterialization to treat patients suffering from symptomatic hemorrhoids. Methods: The procedure consists of the closure of the terminal branches of the superior rectal artery approximately 2-3 cm above the dentate line by means of laser shots originated by a diode laser platform. The arteries, at that level, have variable location and distribution. Therefore, a doppler probe set at the frequency of 20MHz helps identifying the arteries that would be missed otherwise. The laser beam is well tolerated by patients. For this reason, anesthesia is not required in most cases and the procedure allows a quick return to daily activities. In case of concomitant severe mucosal prolapse, the laser treatment can be combined with suture mucopexy. Three to six running sutures allow a complete lifting of hemorrhoidal piles, securing long-term resolution of symptoms. Results and Conclusions: ‘HeLP’ is indicated in patients with symptomatic hemorrhoids where conservative treatment failed and when mucosal prolapse is scarce or not symptomatic. The addition of mucopexy to laser treatment (HeLPexx) contributes to overall resolution of symptoms when mucosal prolapse is an issue, Emborrhoid is another novel, ‘hi-tech’ form of selective dearterialization used in selected case of hemorrhoids where main symptom is bleeding. It is generally used in cases where surgery is contraindicated due to severe concomitant diseases.

scholarly journals A target enrichment probe set for resolving the flagellate land plant tree of life

2021 ◽  
Vol 9 (1) ◽  
Author(s):  
Jesse W. Breinholt ◽  
Sarah B. Carey ◽  
George P. Tiley ◽  
E. Christine Davis ◽  
Lorena Endara ◽  
...  
Keyword(s):  
Land Plant ◽  
Tree Of Life ◽  
Target Enrichment ◽  
Probe Set

Corrosion surveillance program for tank, fuel cladding and supporting structure of 30 MW Indonesian RSG GAS research reactor

Kerntechnik ◽  
2021 ◽  
Vol 86 (3) ◽  
pp. 236-243
Author(s):  
G. R. Sunaryo ◽  
R. Kusumastuti ◽  
Sriyono
Keyword(s):  
Water Quality ◽  
Power Plants ◽  
Research Reactor ◽  
Visual Analysis ◽  
Galvanic Corrosion ◽  
Steel Wire ◽  
Chloride Ions ◽  
Bottom Surface ◽  
Surveillance Program ◽  
Probe Set

Abstract The objective of this research is to understand the condition of the structural material of the 30 MW RSG-GAS research reactor as input for the aging management program. Furthermore, this should enable a prediction of the remaining life of the components. In the current experiment, corrosion surveillance was carried out at Interim Storage for Spent Fuel (ISSF), that has similar water quality as in reactor pool by using a corrosion probe which is made of aluminum alloy and stainless steel. The probe set is designed to understand the effect of water quality in the ISSF pond. The corrosion processes observed were pitting, crevice and galvanic corrosion. Two sets of corrosion probes were immersed into the ISSF pool in 2007, hanging by steel wire, 1-meter height from the bottom surface. One probe set consists of horizontal and vertical positions. The soaking time was 7 years. The observations made were water chemical content, corrosion rate and visual analysis, macro and micro. For macro visual observations an optical microscope was used, for micro-observations SEM-EDX. From the results of macro-observations, information on the presence of galvanic corrosion, crevice and pitting was obtained. SEM-EDX provides information on the influence of chloride ions on corrosion products. This experience will be very useful in dealing with the aging process of Indonesia’s nuclear power plants in the future.

A novel exome probe set captures phototransduction genes across birds (Aves) enabling efficient analysis of vision evolution

2021 ◽  
Author(s):  
Noor D. White ◽  
Zachary A. Batz ◽  
Edward L. Braun ◽  
Michael J. Braun ◽  
Karen L. Carleton ◽  
...  
Keyword(s):  
Probe Set

scholarly journals A Universal Probe Set for Targeted Sequencing of 353 Nuclear Genes from Any Flowering Plant Designed Using k-Medoids Clustering

2018 ◽  
Vol 68 (4) ◽  
pp. 594-606 ◽  
Author(s):  
Matthew G Johnson ◽  
Lisa Pokorny ◽  
Steven Dodsworth ◽  
Laura R Botigué ◽  
Robyn S Cowan ◽  
...  
Keyword(s):  
Nuclear Genes ◽  
Targeted Sequencing ◽  
Flowering Plant ◽  
Probe Set

scholarly journals Oligonucleotide capture sequencing of the SARS-CoV-2 genome and subgenomic fragments from COVID-19 individuals

2020 ◽  
Author(s):  
Harsha Doddapaneni ◽  
Sara Javornik Cregeen ◽  
Richard Sucgang ◽  
Qingchang Meng ◽  
Xiang Qin ◽  
...  
Keyword(s):  
Transcriptional Activity ◽  
Oligonucleotide Probe ◽  
Threshold Value ◽  
Open Reading Frames ◽  
Clinical Samples ◽  
Viral Loads ◽  
Full Length Genome ◽  
Probe Set ◽  
Reading Frames ◽  
Capture Sequencing

AbstractThe newly emerged and rapidly spreading SARS-CoV-2 causes coronavirus disease 2019 (COVID-19). To facilitate a deeper understanding of the viral biology we developed a capture sequencing methodology to generate SARS-CoV-2 genomic and transcriptome sequences from infected patients. We utilized an oligonucleotide probe-set representing the full-length genome to obtain both genomic and transcriptome (subgenomic open reading frames [ORFs]) sequences from 45 SARS-CoV-2 clinical samples with varying viral titers. For samples with higher viral loads (cycle threshold value under 33, based on the CDC qPCR assay) complete genomes were generated. Analysis of junction reads revealed regions of differential transcriptional activity and provided evidence of expression of ORF10. Heterogeneous allelic frequencies along the 20kb ORF1ab gene suggested the presence of a defective interfering viral RNA species subpopulation in one sample. The associated workflow is straightforward, and hybridization-based capture offers an effective and scalable approach for sequencing SARS-CoV-2 from patient samples.

scholarly journals An optimized ribodepletion approach for C. elegans RNA-sequencing libraries

2021 ◽  
Author(s):  
Alec Barrett ◽  
Rebecca McWhirter ◽  
Seth R Taylor ◽  
Alexis Weinreb ◽  
David M Miller ◽  
...  
Keyword(s):  
Ribosomal Rna ◽  
Noncoding Rnas ◽  
Rrna Gene ◽  
Library Preparation ◽  
C Elegans ◽  
Pcr Duplicates ◽  
Rrna Depletion ◽  
Dissociated Cells ◽  
Probe Set ◽  
Long Genes

ABSTRACTA recent and powerful technique is to obtain transcriptomes from rare cell populations, such as single neurons in C. elegans, by enriching dissociated cells using fluorescent sorting. However, these cell samples often have low yields of RNA that present challenges in library preparation. This can lead to PCR duplicates, noisy gene expression for lowly expressed genes, and other issues that limit endpoint analysis. Further, some common resources, such as sequence specific kits for removing ribosomal RNA, are not optimized for non-mammalian samples. To optimize library construction for such challenging samples, we compared two approaches for building RNAseq libraries from less than 10 nanograms of C. elegans RNA: SMARTSeq V4 (Takara), a widely used kit for selecting poly-adenylated transcripts; and SoLo Ovation (Tecan Genomics), a newly developed ribodepletion-based approach. For ribodepletion, we used a custom kit of 200 probes designed to match C. elegans rRNA gene sequences. We found that SoLo Ovation, in combination with our custom C. elegans probe set for rRNA depletion, detects an expanded set of noncoding RNAs, shows reduced noise in lowly expressed genes, and more accurately counts expression of long genes. The approach described here should be broadly useful for similar efforts to analyze transcriptomics when RNA is limiting.

Evaluation of droplet digital PCR for the detection of black canker disease in tomato

Plant Disease ◽  
2021 ◽  
Author(s):  
Li Wang ◽  
Tian Qian ◽  
Pei Zhou ◽  
Wenjun Zhao ◽  
Xianchao Sun
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Plant Pathogens ◽  
Plant Protection ◽  
Detection Threshold ◽  
Digital Pcr ◽  
Taqman Probe ◽  
Tomato Seed ◽  
Canker Disease ◽  
Probe Set

Clavibacter michiganensis subsp. michiganensis (Cmm), the cause of bacterial canker disease, is one of the most destructive pathogens in greenhouse and field tomato. The pathogen is now present in all main production areas of tomato and is quite widely distributed in the EPPO（European and Mediterranean Plant Protection Organization）region. The inspection and quarantine of the plant pathogens relies heavily on accurate detection tools. Primers and probes reported in previous studies do not distinguish the Cmm pathogen from other closely related subspecies of C. michiganensis, especially the non-pathogenic subspecies that were identified from tomato seeds recently. Here, we have developed a droplet digital polymerase chain reaction (ddPCR) method for the identification of this specific bacterium with primers/TaqMan probe set designed based on the pat-1 gene of Cmm. This new primers/probe set has been evaluated by qPCRthe real time PCR（qPCR） and ddPCR. The detection results suggest that the ddPCR method established in this study was highly specific for the target strains. The result showed the positive amplification for all 5 Cmm strains，and no amplification was observed for the other 43 tested bacteria, including the closely related C. michiganensis strains. The detection threshold of ddPCR was 10.8 CFU/mL for both pure Cmm cell suspensions and infected tomato seed, which was 100 times-fold more sensitive than that of the real-time PCR (qPCR ) performed using the same primers and probe. The data obtained suggest that our established ddPCR could detect Cmm even with low bacteria load, which could facilitate both Cmm inspection for pathogen quarantine and the routine pathogen detection for disease control of black canker in tomato.

Affymetrix Chip Definition Files Construction Based on Custom Probe Set Annotation Database

2011 ◽  
pp. 135-144 ◽  
Author(s):  
Michał Marczyk ◽  
Roman Jaksik ◽  
Andrzej Polański ◽  
Joanna Polańska
Keyword(s):  
Annotation Database ◽  
Affymetrix Chip ◽  
Probe Set

scholarly journals Modified Method to Detect PCR Products by 5′Nuclease Activity and an Asymmetric Fluorogenic Probe Set

BioTechniques ◽  
2000 ◽  
Vol 29 (4) ◽  
pp. 690-694 ◽  
Author(s):  
Kai Koo ◽  
Lee-Ann Jaykus
Keyword(s):  
Nuclease Activity ◽  
Modified Method ◽  
Fluorogenic Probe ◽  
Pcr Products ◽  
Probe Set
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