A guide for single‐particle chromatin tracking in live cell nuclei

2022 ◽  
Author(s):  
Mengdi Zhang ◽  
Clayton Seitz ◽  
Garrick Chang ◽  
Fadil Iqbal ◽  
Hua Lin ◽  
...  
Keyword(s):  
Single Particle ◽  
Live Cell ◽  
Cell Nuclei

scholarly journals Simultaneous AFM and FLIM Imaging with a SiR-DNA Probe Reveals Structural Changes during DNA Condensation in Live Cell Nuclei

2018 ◽  
Vol 114 (3) ◽  
pp. 596a
Author(s):  
Chetan Poudel ◽  
Nathan Curry ◽  
Kevin A. Feeney ◽  
Gabriele S. Kaminski Schierle ◽  
Clemens F. Kaminski
Keyword(s):  
Structural Changes ◽  
Dna Condensation ◽  
Dna Probe ◽  
Live Cell ◽  
Cell Nuclei

Non-rigid multi-frame registration of cell nuclei in live cell fluorescence microscopy image data

2015 ◽  
Vol 19 (1) ◽  
pp. 1-14 ◽  
Author(s):  
Marco Tektonidis ◽  
Il-Han Kim ◽  
Yi-Chun M. Chen ◽  
Roland Eils ◽  
David L. Spector ◽  
...  
Keyword(s):  
Fluorescence Microscopy ◽  
Image Data ◽  
Live Cell ◽  
Cell Nuclei ◽  
Microscopy Image

scholarly journals Lysosome Mobility Probed with Live Cell Imaging and Single Particle Tracking

2013 ◽  
Vol 104 (2) ◽  
pp. 651a
Author(s):  
Debjyoti Bandyopadhyay ◽  
Youngeun J. Kim ◽  
Jairo Zapata ◽  
Christine K. Payne
Keyword(s):  
Particle Tracking ◽  
Single Particle ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Single Particle Tracking ◽  
Live Cell

scholarly journals Correction to Study of RNA Polymerase II Clustering inside Live-Cell Nuclei Using Bayesian Nanoscopy

ACS Nano ◽  
2016 ◽  
Vol 10 (4) ◽  
pp. 4882-4882 ◽  
Author(s):  
Xuanze Chen ◽  
Mian Wei ◽  
M. Mocarlo Zheng ◽  
Jiaxi Zhao ◽  
Huiwen Hao ◽  
...  
Keyword(s):  
Rna Polymerase ◽  
Rna Polymerase Ii ◽  
Live Cell ◽  
Cell Nuclei

scholarly journals Coloring Single Nanoparticle Trajectory in Live Cell with its Own History: a Presuppositionless Preprocessing Approach

2019 ◽  
Author(s):  
Hansen Zhao ◽  
Zhenrong Huang ◽  
Feng Ge ◽  
Xiangjun Shi ◽  
Bin Xiong ◽  
...  
Keyword(s):  
Single Particle ◽  
Complex Dynamics ◽  
Live Cell ◽  
State Transitions ◽  
Human Beings ◽  
Global Pattern ◽  
Model Free ◽  
Mechanical Models ◽  
Data Points ◽  
Underlying Mechanisms

AbstractAnalyzing single particle trajectories is a prominent issue in understanding complex dynamics such as nanoparticle-cell interactions. Existing methods treat data points as isolated “atoms” and use predefined mechanical models to “frame” their complicated relationship. Herein, we propose a “historical evolution” based model-free strategy. It allows spatiotemporal heterogeneity embedded in a trajectory to self-emerge as consecutive colored segments before any model assumption, provide both an overall picture and local state transitions on the particle movement with minimum information loss, and inspire further model-based investigation. We demonstrate with simulations and experiments that the underlying mechanisms of various time-series and motion states of single nanoparticles on live cell membranes could all be revealed successfully. Since complexity studies at different levels of molecules, particles, cells, human beings, vehicles, and even stars could all be reduced to analyzing spatiotemporal trajectories of “single particles”, this presuppositionless approach will help fundamental researches on many important systems.Impact StatementA preprocessing strategy for single particle trajectory analysis is established by providing an intuitive global pattern from “historical experiences” of the particle without predefining any mechanical models.

scholarly journals Author response: Live-cell single particle imaging reveals the role of RNA polymerase II in histone H2A.Z eviction

2020 ◽  
Author(s):  
Anand Ranjan ◽  
Vu Q Nguyen ◽  
Sheng Liu ◽  
Jan Wisniewski ◽  
Jee Min Kim ◽  
...  
Keyword(s):  
Rna Polymerase ◽  
Rna Polymerase Ii ◽  
Single Particle ◽  
Live Cell ◽  
Author Response ◽  
Particle Imaging ◽  
Single Particle Imaging

scholarly journals Live‐cell imaging of octaarginine‐modified polymer dots via single particle tracking

2019 ◽  
Vol 52 (2) ◽  
pp. e12556 ◽  
Author(s):  
Yao Luo ◽  
Yuping Han ◽  
Xingjie Hu ◽  
Min Yin ◽  
Changfeng Wu ◽  
...  
Keyword(s):  
Particle Tracking ◽  
Single Particle ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Single Particle Tracking ◽  
Live Cell ◽  
Modified Polymer ◽  
Polymer Dots
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