scholarly journals Simultaneous AFM and FLIM Imaging with a SiR-DNA Probe Reveals Structural Changes during DNA Condensation in Live Cell Nuclei

2018 ◽  
Vol 114 (3) ◽  
pp. 596a
Author(s):  
Chetan Poudel ◽  
Nathan Curry ◽  
Kevin A. Feeney ◽  
Gabriele S. Kaminski Schierle ◽  
Clemens F. Kaminski
Keyword(s):  
Structural Changes ◽  
Dna Condensation ◽  
Dna Probe ◽  
Live Cell ◽  
Cell Nuclei

Structural Changes During The Initiation Of Pollen Embryogenesis In Barley: Ultrastructure Analysis And Live Cell Imaging

2012 ◽  
Vol 18 (S2) ◽  
pp. 258-259
Author(s):  
M. Melzer
Keyword(s):  
Live Cell Imaging ◽  
Structural Changes ◽  
Cell Imaging ◽  
Live Cell ◽  
Pollen Embryogenesis

Extended abstract of a paper presented at Microscopy and Microanalysis 2012 in Phoenix, Arizona, USA, July 29 – August 2, 2012.

scholarly journals Prenatal tracheal ligation or intra-amniotic administration of surfactant or dexamethasone prevents some structural changes in the pulmonary arteries of surgically created diaphragmatic hernia in rabbits

2002 ◽  
Vol 57 (1) ◽  
pp. 1-8 ◽  
Author(s):  
Consuelo J. Rodrigues ◽  
Uenis Tannuri ◽  
Ana Cristina A. Tannuri ◽  
João Maksoud-Filho ◽  
Aldo J. Rodrigues Junior
Keyword(s):  
Congenital Diaphragmatic Hernia ◽  
Diaphragmatic Hernia ◽  
Structural Changes ◽  
Elastic Fiber ◽  
Pulmonary Arteries ◽  
External Diameter ◽  
Cell Nuclei ◽  
Fiber Density ◽  
Positive Effects ◽  
Tracheal Ligation

PURPOSE: Characterization of the structural changes occurring in the pulmonary arteries resulting from surgically produced congenital diaphragmatic hernia in rabbits, with particular emphasis on the preventive effects of prenatal tracheal ligation or administration of intra-amniotic dexamethasone or surfactant. METHODS: Twenty rabbit fetuses underwent surgical creation of a left-sided congenital diaphragmatic hernia on the 24th or 25th gestational day. They were divided according to the following procedures: congenital diaphragmatic hernia (n = 5), congenital diaphragmatic hernia plus tracheal ligation (n = 5), congenital diaphragmatic hernia plus intra-amniotic administration of dexamethasone 0.4 mg (n = 5) or surfactant (Curosurf 40 mg, n = 5). On gestational day 30, all the fetuses were delivered by caesarean section and killed. A control group consisted of five nonoperated fetuses. Histomorphometric analysis of medial thickness, cell nuclei density, and elastic fiber density of pulmonary arterial walls was performed. RESULTS: Arteries with an external diameter > 100 mum have a decreased medial thickness, lower cell nuclei density, and greater elastic fiber density when compared with arteries with external diameter <= 100 mum. Congenital diaphragmatic hernia promoted a significant decrease in medial thickness and an increase in cell nuclei density in artery walls with external diameter > 100 mum. Prenatal treatments with tracheal ligation or intra-amniotic administration of dexamethasone or surfactant prevented these changes. In arteries with external diameter <= 100 mum, congenital diaphragmatic hernia promoted a significant increase in medial thickness and in cell nuclei density and a decrease in elastic fiber density. The prenatal treatments with tracheal ligation or intra-amniotic administration of dexamethasone or surfactant prevented these changes, although no effect was observed in elastic fiber density in the congenital diaphragmatic hernia plus dexamethasone group. CONCLUSIONS: Congenital diaphragmatic hernia promoted different structural changes for large or small arteries. The prenatal intra-amniotic administration of dexamethasone or surfactant had positive effects on the lung structural changes promoted by congenital diaphragmatic hernia, and these effects were comparable to the changes induced by tracheal ligation.

Non-rigid multi-frame registration of cell nuclei in live cell fluorescence microscopy image data

2015 ◽  
Vol 19 (1) ◽  
pp. 1-14 ◽  
Author(s):  
Marco Tektonidis ◽  
Il-Han Kim ◽  
Yi-Chun M. Chen ◽  
Roland Eils ◽  
David L. Spector ◽  
...  
Keyword(s):  
Fluorescence Microscopy ◽  
Image Data ◽  
Live Cell ◽  
Cell Nuclei ◽  
Microscopy Image

scholarly journals Correction to Study of RNA Polymerase II Clustering inside Live-Cell Nuclei Using Bayesian Nanoscopy

ACS Nano ◽  
2016 ◽  
Vol 10 (4) ◽  
pp. 4882-4882 ◽  
Author(s):  
Xuanze Chen ◽  
Mian Wei ◽  
M. Mocarlo Zheng ◽  
Jiaxi Zhao ◽  
Huiwen Hao ◽  
...  
Keyword(s):  
Rna Polymerase ◽  
Rna Polymerase Ii ◽  
Live Cell ◽  
Cell Nuclei

scholarly journals Chomatin mass from previously aggregated, pyknotic, and fragmented monolayer nuclei is a source for dome cell nuclei generated by amitosis: Differentiation of Ishikawa Domes, Part 3

2016 ◽  
Author(s):  
Honoree Fleming
Keyword(s):  
Epithelial Cells ◽  
Nuclear Structure ◽  
Cell Membranes ◽  
Structural Changes ◽  
Cell Nuclei ◽  
Vacuole Formation ◽  
Hematoxylin Staining ◽  
Cavity Filling ◽  
Irregular Mass ◽  
Endometrial Epithelial Cells

Ishikawa endometrial epithelial cells are capable of differentiation from monolayer cells into fluid-enclosing hemispheres through a surprisingly complex series of structural changes as discussed in this and in two accompanying papers (Fleming, 2016a; Fleming 2016b). The process starts with the dissolution of cell membranes in defined regions throughout a monolayer that has been stimulated to differentiate (Fleming, 1995). Aggregated nuclei become wrapped in membranes containing mitochondrial carboxylases, and apparently generated by contiguous mitochondria. These mitonucleons are involved in vacuole formation that elevates the syncytium into a predome (Fleming, 2015a). The mitonucleons begin to fall apart several hours after formation as the enveloping membranes are breached and the pyknotic chromatin undergoes profound changes (Fleming, 2015b). Chromatin deconstruction, with attendant disappearance of the typical ovoid nuclear structure, results in chromatin fibers that fill the envelope formed by the apical and basal membranes of the syncytium, now stretching over a cavity filling with fluid. In the next several hours, hematoxylin staining, greatly diminished when nuclei were fragmented, reappears in an irregular mass of chromatin out of which nuclei form amitotically and increase in numbers until they fill the envelope. Subsequently cell membranes form around the nuclei. Domes can enlarge and even extend into tubules by becoming vacuolized and undergoing the same amitotic process that created the dome initially.

scholarly journals Excess copper induces structural changes in cultured photosynthetic soybean cells

2006 ◽  
Vol 33 (11) ◽  
pp. 1001 ◽  
Author(s):  
María Bernal ◽  
Pilar Sánchez-Testillano ◽  
María del Carmen Risueño ◽  
Inmaculada Yruela
Keyword(s):  
Cell Wall ◽  
Structural Changes ◽  
Copper Tolerance ◽  
Copper Stress ◽  
Induced Response ◽  
Cytoplasmic Vacuole ◽  
Cell Nuclei ◽  
Short Term ◽  
Excess Copper

Soybean [Glycine max (L.) Merr.] cell suspensions have the capacity to develop tolerance to excess copper, constituting a convenient system for studies on the mechanisms of copper tolerance. The functional cell organisation changes observed in these cell cultures after both short-term (stressed cells) and long-term (acclimated cells) exposure to 10 μm CuSO4 are reported from structural, cytochemical and microanalytical approaches. Cells grown in the presence of 10 μm CuSO4 shared some structural features with untreated cells, such as: (i) a large cytoplasmic vacuole, (ii) chloroplasts along the thin layer of cytoplasm, (iii) nucleus in a peripheral location exhibiting circular-shaped nucleolus and a decondensed chromatin pattern, and (iv) presence of Cajal bodies in the cell nuclei. In addition, cells exposed to 10 μm CuSO4 exhibited important differences compared with untreated cells: (i) chloroplasts displayed rounded shape and smaller size with denser-structured internal membranes, especially in copper-acclimated cells; (ii) no starch granules were found within chloroplasts; (iii) the cytoplasmic vacuole was larger, especially after long-term copper exposure; (iv) the levels of citrate and malate increased. Extracellular dark-coloured deposits with high copper content attached at the outer surface of the cell wall were observed only in cells exposed to a short-term copper stress. Structural cell modifications, mainly affecting chloroplasts, accompanied the short-term copper-induced response and were maintained as stable characters during the period of adaptation to excess copper. Vacuolar changes accompanied the long-term copper response. The results indicate that the first response of soybean cells to excess copper prevents its entry into the cell by immobilising it in the cell wall, and after an adaptive period, acclimation to excess copper may be mainly due to vacuolar sequestration.

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