What are the effects of adding piperonyl butoxide (PBO) to pyrethroid-treated nets for prevention of malaria?

The purpose of the research is development of preventive measures against zooanthroponoze vector-borne diseases spread by parasitic arthropods in the Kaluga Region. Materials and methods. The subject of the research was Ixodidae, mosquitoes, and small mammals inhabiting the Kaluga Region. The census of parasitic arthropods was carried out on the territory of all districts of the Kaluga Region and the city of Kaluga. Open natural habitat and human settlements were investigated. Weather conditions from 2013 to 2018 were also taken into account. For the purposes of the study, we used standard methods for capturing and counting arthropods and mouse-like rodents. In order to obtain mathematical models of small mammal populations, a full factorial experiment was conducted using the collected statistical data. In-process testing of the drug based on s-fenvalerate and piperonyl butoxide were carried out under the conditions of the agricultural collective farm “Niva” of the Kozelsky District, the Kaluga Region, and LLC “Angus Center of Genetics” of the Babyninsky District, the Kaluga Region. Results and discussion. In the Kaluga Region, two species of ixodic ticks are found, namely, Ixodes ricinus and Dermacentor reticulatus, which have two activity peaks. Mosquito may have 3-4 generations in a year in the Kaluga region. The most common mosquito species in the Kaluga Region are Aedes communis, Ae. (Och.) togoi and Ae. (Och.) diantaeus, Culex pipiens Culex Linnaeus, 1758 (Diptera, Culicidae) (Culex pipiens): Cx. pipiens f. pipiens L. (non-autogenic form) and Cx. p. f. molestus Fors. (autogenic form), which interbreed, and reproductively isolated in the Region. The developed mathematical models make it possible to quantify the risks of outbreaks of zooanthroponoze vector-borne diseases without the cost of field research, and allow for rational, timely and effective preventive measures. Medications based on s-fenvalerate and piperonyl butoxide and based on cyfluthrin showed high insecto-acaricidal efficacy and safety.

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Escalation of Pyrethroid Resistance in the Malaria Vector Anopheles funestus Induces a Loss of Efficacy of Piperonyl Butoxide–Based Insecticide-Treated Nets in Mozambique

The Journal of Infectious Diseases ◽

10.1093/infdis/jiz139 ◽

2019 ◽

Vol 220 (3) ◽

pp. 467-475 ◽

Cited By ~ 16

Author(s):

Jacob M Riveron ◽

Silvie Huijben ◽

Williams Tchapga ◽

Magellan Tchouakui ◽

Murielle J Wondji ◽

...

Keyword(s):

Cytochrome P450 ◽

Pyrethroid Resistance ◽

Anopheles Funestus ◽

Resistance Mechanisms ◽

Piperonyl Butoxide ◽

Insecticide Treated Nets ◽

Molecular Analyses ◽

Cytochrome P450 Genes ◽

High Degree ◽

Long Lasting Insecticidal Nets

Abstract Background Insecticide resistance poses a serious threat to insecticide-based interventions in Africa. There is a fear that resistance escalation could jeopardize malaria control efforts. Monitoring of cases of aggravation of resistance intensity and its impact on the efficacy of control tools is crucial to predict consequences of resistance. Methods The resistance levels of an Anopheles funestus population from Palmeira, southern Mozambique, were characterized and their impact on the efficacy of various insecticide-treated nets established. Results A dramatic loss of efficacy of all long-lasting insecticidal nets (LLINs), including piperonyl butoxide (PBO)–based nets (Olyset Plus), was observed. This An. funestus population consistently (2016, 2017, and 2018) exhibited a high degree of pyrethroid resistance. Molecular analyses revealed that this resistance escalation was associated with a massive overexpression of the duplicated cytochrome P450 genes CYP6P9a and CYP6P9b, and also the fixation of the resistance CYP6P9a_R allele in this population in 2016 (100%) in contrast to 2002 (5%). However, the low recovery of susceptibility after PBO synergist assay suggests that other resistance mechanisms could be involved. Conclusions The loss of efficacy of pyrethroid-based LLINs with and without PBO is a concern for the effectiveness of insecticide-based interventions, and action should be taken to prevent the spread of such super-resistance.

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Different Pathways of Constitutive Androstane Receptor–mediated Liver Hypertrophy and Hepatocarcinogenesis in Mice Treated with Piperonyl Butoxide or Decabromodiphenyl Ether

Toxicologic Pathology ◽

10.1177/0192623313482055 ◽

2013 ◽

Vol 41 (8) ◽

pp. 1078-1092 ◽

Cited By ~ 11

Author(s):

Yohei Sakamoto ◽

Kaoru Inoue ◽

Miwa Takahashi ◽

Yoshikazu Taketa ◽

Yukio Kodama ◽

...

Keyword(s):

Constitutive Androstane Receptor ◽

Piperonyl Butoxide ◽

Decabromodiphenyl Ether

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168 The effects of fly control strategies on fly abundance, fly avoidance behaviors, and performance in feedlot bulls and steers

Journal of Animal Science ◽

10.1093/jas/skz397.034 ◽

2020 ◽

Vol 98 (Supplement_2) ◽

pp. 15-16

Author(s):

Courtney McNeff ◽

Justin Talley ◽

Colton A Robison ◽

Blake K Wilson

Keyword(s):

Treatment Time ◽

Control Strategies ◽

Block Design ◽

Animal Health ◽

Piperonyl Butoxide ◽

Stable Fly ◽

Animal Performance ◽

Time Interaction ◽

Avoidance Behaviors ◽

Fly Control

Abstract The objective of this experiment was to examine the effects of fly control strategies on fly abundance, fly avoidance behaviors, and animal performance in a feedlot setting. Angus bulls (n = 64; BW = 281 ± 36.2 kg) and steers (n = 36; BW = 475 ± 40.4 kg) were blocked by sex and BW and assigned to 1 of 4 experimental treatments in a randomized complete block design (7 pens/treatment; 4 bulls/pen; 3 steers/pen). Treatments included: control (CON; no fly control), abamectin, piperonyl butoxide, and zetacypermethrin insecticide tags (FT; Tri-Zap®, Y-Tex Corporation, Cody, WY), permethrin and piperonyl butoxide pour on (PO; Permectrin® CDS, Bayer Animal Health, Shawnee Mission, KS), or a garlic-powder top dress (GR) administered at 0.28 g •-1 animal •-1d. The experiment was conducted between June and September. Horn and stable fly abundance (FA) and fly avoidance behaviors (FAB; i.e. tail-flicks, stomping, head-tossing) were recorded by trained personnel. Both FA and FAB were recorded once/wk on days with similar temperatures, humidity, wind speed, and without precipitation based on Oklahoma Mesonet data (Stillwater site). There was a tendency for a treatment × time interaction for horn FA with differences in treatments occurring at wk 1, 2, 8, and 14. There was no treatment × time interaction for stable FA or FAB, however both were affected by time (P < 0.001). There were no differences in final BW (P = 0.34), overall ADG (P = 0.30), overall DMI (P = 0.53), or overall G:F (P = 0.39). There was a tendency (P ≤ 0.10) for GR to have decreased BW on d 28 and decreased ADG from d 0 to 28 compared to FT and PO. There was also a tendency (P = 0.09) for decreased DMI from d 0 to 56 for GR compared to CON and FT. Overall FA was less than expected (mean = 1 to 17 flies/animal) throughout the experiment and overall animal performance was unaffected by fly control strategy.

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The Effect of Piperonyl Butoxide and Adjuvants on Sulfonylurea Herbicide Activity

Weed Technology ◽

10.1017/s0890037x00045826 ◽

1996 ◽

Vol 10 (1) ◽

pp. 127-133 ◽

Cited By ~ 1

Author(s):

Chae Soon Kwon ◽

Donald Penner

Keyword(s):

Seed Oil ◽

Oxidase Inhibitor ◽

Piperonyl Butoxide ◽

Mixed Function Oxidase ◽

Sulfonylurea Herbicide ◽

Weed Species ◽

Common Lambsquarters ◽

Giant Foxtail ◽

Herbicide Activity ◽

Grass Weed

Greenhouse studies showed that the mixed function oxidase inhibitor, piperonyl butoxide (PBO), tank-mixed with the sulfonylurea herbicides, nicosulfuron, primisulfuron, and thifensulfuron, in the absence of effective adjuvants enhanced herbicide activity on both broadleaf and grass weed species. Effective adjuvants for nicosulfuron were K-3000 for common lambsquarters, Sylgard® 309 Surfactant for velvetleaf, K-2000 for barnyardgrass, and K-2000, K-3000, and Scoil® methylated seed oil for giant foxtail control. K-3000 and Sylgard 309 enhanced velvetleaf control with primisulfuron and thifensulfuron. The 28% urea and ammonium nitrate (UAN) was more effective as an adjuvant with thifensulfuron for velvetleaf than for common lambsquarters control. The enhancement of sulfonylurea herbicide activity with PBO was most apparent when other adjuvants were least effective.

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