Decision risk approach to the application of biological indicators in vapor phased hydrogen peroxide bio-decontamination

2018 ◽  
Vol 32 (4) ◽  
pp. e3015
Author(s):  
Éva Pusztai ◽  
Sándor Kemény ◽  
Emese Vágó ◽  
Anikó Horváth
Keyword(s):  
Hydrogen Peroxide ◽  
Biological Indicators ◽  
Risk Approach

A Head-to-Head Comparison of Hydrogen Peroxide Vapor and Aerosol Room Decontamination Systems

2011 ◽  
Vol 32 (9) ◽  
pp. 831-836 ◽  
Author(s):  
T. Holmdahl ◽  
P. Lanbeck ◽  
M. Wullt ◽  
M. H. Walder
Keyword(s):  
Hydrogen Peroxide ◽  
Cycle Time ◽  
New Technologies ◽  
Biological Indicators ◽  
The Other ◽  
Test Room ◽  
Cycle Times ◽  
Hydrogen Peroxide Vapor ◽  
In Vitro Comparison

Objective.New technologies have emerged in recent years for the disinfection of hospital rooms and equipment that may not be disinfected adequately using conventional methods. There are several hydrogen peroxide–based area decontamination technologies on the market, but no head-to-head studies have been performed.Design.We conducted a head-to-head in vitro comparison of a hydrogen peroxide vapor (HPV) system (Bioquell) and an aerosolized hydrogen peroxide (aHP) system (Sterinis).Setting.The tests were conducted in a purpose-built 136-m3test room.Methods.One HPV generator and 2 aHP machines were used, following recommendations of the manufacturers. Three repeated tests were performed for each system. The microbiological efficacy of the 2 systems was tested using 6-log Tyvek-pouchedGeobacillus stearo-thermophilusbiological indicators (BIs). The indicators were placed at 20 locations in the first test and 14 locations in the subsequent 2 tests for each system.Results.All BIs were inactivated for the 3 HPV tests, compared with only 10% in the first aHP test and 79% in the other 2 aHP tests. The peak hydrogen peroxide concentration was 338 ppm for HPV and 160 ppm for aHP. The total cycle time (including aeration) was 3 and 3.5 hours for the 3 HPV tests and the 3 aHP tests, respectively. Monitoring around the perimeter of the enclosure with a handheld sensor during tests of both systems did not identify leakage.Conclusion.One HPV generator was more effective than 2 aHP machines for the inactivation ofG. stearothermophilusBIs, and cycle times were faster for the HPV system.

Effectiveness of Vaporous Hydrogen Peroxide for the Decontamination of Bacillus atrophaeus in Confined Space

2014 ◽  
Vol 912-914 ◽  
pp. 1928-1931
Author(s):  
Ting Liang ◽  
Kan Zhou ◽  
Jin Gu ◽  
Rui Yan ◽  
Zhuang Zhou Ji
Keyword(s):  
Hydrogen Peroxide ◽  
Biological Indicators ◽  
Technical Standard ◽  
Confined Space ◽  
Bacillus Atrophaeus ◽  
Interior Space ◽  
Room Volume

The main objective of this study was to make out the decontamination effects of bacillus atrophaeus in different locations by Vaporous Hydrogen Peroxide decontamination equipment which was designed independently. Bacillus atrophaeus were in different locations of the room, the room volume is 67m3. Then the Vaporous Hydrogen Peroxide (VHP) decontamination equipment was opened to sterilize the room. After the sterilization, based on the Technical Standard For disinfection, the biological indicators including bacillus atrophaeus were incubated at37 °C for 48 hr,resultant colonies were enumerated. The BI results showed that along with the increase of distance between VHP decontamination equipment and the BI, decontamination time gradually extended. When the distance were 2m, 4m and 6m, the decontamination time were 15min, 30min and 30min. When the BI was put in the room corner, the decontamination time was 60min. From this paper,it can be determined that the VHP technology is a valid approach to the sterilization of interior space.

scholarly journals Hybrid Hydrogen Peroxide for Viral Disinfection

2021 ◽  
Author(s):  
Meaghan Hislop ◽  
Frances Grinstead ◽  
John R. Henneman
Keyword(s):  
Hydrogen Peroxide ◽  
Health And Safety ◽  
Biological Indicators ◽  
High Concentration ◽  
Pathogen Elimination ◽  
Recent Innovation ◽  
History Of ◽  
Level 3 ◽  
Good Laboratory ◽  
High Level

Decontamination is often necessary in facilities with sensitive spaces where pathogen elimination is critical. Historically, high concentration vaporized hydrogen peroxide technologies have been applied in these areas for pathogen disinfection. While effective, these high concentration solutions come with inherent risks to human health and safety. Alternatively, one recent innovation is a hybrid hydrogen peroxide system which combines a 7% hydrogen peroxide solution with a calibrated fogging device that delivers a mixture of vaporous and micro aerosolized particles, significantly lowering the risk of exposure to high-concentration hazardous chemicals. Studies performed with this technology demonstrate high level pathogen decontamination across a variety of tested pathogens and substrates. This chapter will cover a brief history of hydrogen peroxide technologies and their application processes; examine the correlations between viral inactivation, viral disinfection, and biological indicators for validation; demonstrate the necessity of dwell time for optimal efficacy; discuss the effects of viral disinfectant use on laboratory surfaces; and examine various studies, including virologic work performed in Biosafety Level 3 facilities and good laboratory practice (GLP) data performed by EPA-approved laboratories. This chapter will provide readers a deeper understanding of essential components and considerations when implementing hydrogen peroxide systems for viral decontamination.

The Hitchhiker’s Guide to Hydrogen Peroxide Fumigation, Part 2: Verifying and Validating Hydrogen Peroxide Fumigation Cycles

2020 ◽  
pp. 153567602092109
Author(s):  
Daniel Kümin ◽  
Monika Gsell Albert ◽  
Benjamin Weber ◽  
Kathrin Summermatter
Keyword(s):  
Hydrogen Peroxide ◽  
Relevant Literature ◽  
Biological Indicators ◽  
Physical Parameters ◽  
Technical Requirements ◽  
Chemical Indicators ◽  
Literature Searches ◽  
Personal Experiences ◽  
Long Time ◽  
Process Controls

Introduction: Part 1 of this two-part series describes the use of hydrogen peroxide as a fumigant and compares it with other fumigants on the market. Technical requirements are outlined while considering physical and biological limitations of the system. This second part focuses primarily on the use of process controls to verify and validate hydrogen peroxide fumigations. Finally, a model encompassing the entire fumigation process is presented. Methods: Part 2 of the series focuses on the authors’ long-time personal experiences in room and filter fumigation using various fumigation systems and is supplemented with relevant literature searches. Results: The reader is introduced to the planning and implementation of fumigation process validations. Biological indicators help users develop safe and efficient processes. Chemical indicators can be used as process controls, while measuring physical parameters will help avoid condensation of hydrogen peroxide. How many biological and chemical indicators and what type should be applied for cycle development are additionally explained. Discussion: It is important to consider numerous technical requirements when planning to implement hydrogen peroxide fumigation at an institution. Also, considerable thought needs to go into the verification and validation of the fumigation process. Conclusions: Part 1 of this series presents an overview of different fumigation systems based on hydrogen peroxide on the market and their technical requirements. Part 2 focuses on validation and verification of hydrogen peroxide fumigation while considering the entire fumigation process. The two parts together will serve users as a guide to establishing hydrogen peroxide fumigations at their facilities.

Comparison of the Microbiological Efficacy of Hydrogen Peroxide Vapor and Ultraviolet Light Processes for Room Decontamination

2012 ◽  
Vol 33 (5) ◽  
pp. 507-512 ◽  
Author(s):  
Nancy L. Havill ◽  
Brent A. Moore ◽  
John M. Boyce
Keyword(s):  
Hydrogen Peroxide ◽  
Prospective Observational Study ◽  
Biological Indicators ◽  
Line Of Sight ◽  
Geobacillus Stearothermophilus ◽  
Log Reduction ◽  
Before And After ◽  
The Mean ◽  
Mean Cycle ◽  
Hydrogen Peroxide Vapor

Objective.To compare the microbiological efficacy of hydrogen peroxide vapor (HPV) and ultraviolet radiation (UVC) for room decontamination.Design.Prospective observational study.Setting.500-bed teaching hospital.Methods.HPV and UVC processes were performed in 15 patient rooms. Five high-touch sites were sampled before and after the processes and aerobic colony counts (ACCs) were determined. Carrier disks with ~106Clostridium difficile (CD) spores and biological indicators (BIs) with 104 and 106Geobacillus stearothermophilus spores were placed in 5 sites before decontamination. After decontamination, CD log reductions were determined and BIs were recorded as growth or no growth.Results.93% of ACC samples that had growth before HPV did not have growth after HPV, whereas 52% of sites that had growth before UVC did not have growth after UVC (P < .0001). The mean CD log reduction was >6 for HPV and ~2 for UVC. After HPV 100% of the 104 BIs did not grow, and 22% did not grow after UVC, with a range of 7%–53% for the 5 sites. For the 106 BIs, 99% did not grow after HPV and 0% did not grow after UVC. Sites out of direct line of sight were significantly more likely to show growth after UVC than after HPV. Mean cycle time was 153 (range, 140–177) min for HPV and 73 (range, 39–100) min for UVC (P < .0001).Conclusion.Both HPV and UVC reduce bacterial contamination, including spores, in patient rooms, but HPV is significantly more effective. UVC is significantly less effective for sites that are out of direct line of sight.

scholarly journals Validation of N95 filtering facepiece respirator decontamination methods available at a large university hospital

2020 ◽  
Author(s):  
Krista R. Wigginton ◽  
Peter J. Arts ◽  
Herek Clack ◽  
William J Fitzsimmons ◽  
Mirko Gamba ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Relative Humidity ◽  
Hepatitis Virus ◽  
Biological Indicators ◽  
Biological Indicator ◽  
Filtration Efficiency ◽  
Full Scale ◽  
Moist Heat ◽  
N95 Respirators ◽  
The Impact

AbstractImportanceFiltering facepiece respirators, including N95 masks, are a critical component of infection prevention in hospitals. Due to unprecedented shortages in N95 respirators, many healthcare systems have explored reprocessing of N95 respirators. Data supporting these approaches are lacking in real hospital settings. In particular, published studies have not yet reported an evaluation of multiple viruses, bacteria, and fungi along with respirator filtration and fit in a single, full-scale study.ObjectiveWe initiated a full-scale study to evaluate different N95 FFR decontamination strategies and their impact on respirator integrity and inactivating multiple microorganisms, with experimental conditions informed by the needs and constraints of the hospital.MethodsWe explored several reprocessing methods using new 3M™ 1860 N95 respirators, including dry (<10% relative humidity) and moist (62-66% relative humidity) heat (80-82 °C) in the drying cycle of industrial instrument washers, ethylene oxide (EtO), pulsed xenon UV (UV-PX), hydrogen peroxide gas plasma (HPGP), and vaporous hydrogen peroxide (VHP). Respirator samples were treated and analyzed for biological indicator inactivation using four viruses (MS2, phi6, influenza A virus, murine hepatitis virus), three bacteria (Escherichia coli, Staphylococcus aureus, Geobacillus stearothermophilus), and the fungus Aspergillus niger. The impact of different application media was also evaluated. In parallel, decontaminated respirators were evaluated for filtration integrity and fit.ResultsVHP resulted in >2 log10 inactivation of all tested biological indicators. The combination of UV-PX + moist heat resulted in >2 log10 inactivation of all biological indicators except G. stearothermohphilus. Greater than 95% filtration efficiency was maintained following 2 (UV-PX + <10% relative humidity heat) or 10 (VHP) cycles of treatment, and proper fit was also preserved. UV-PX + dry heat was insufficient to inactivate all biological indicators. Although very effective at virus decontamination, HPGP resulted in decreased filtration efficiency after 3 cycles, and EtO treatment raised potential toxicity concerns. The observed inactivation of viruses with UV-PX, heat, and hydrogen peroxide treatments varied as a function of which culture media (PBS buffer or DMEM) they were deposited in.Conclusions and RelevanceHigh levels of biological indicator inactivation were achieved following treatment with either moist heat or VHP. These same treatments did not significantly impact mask filtration or fit. Hospitals have a variety of scalable options to safely reprocess N95 masks. Beyond value in the current Covid-19 pandemic, the broad group of microorganisms and conditions tested make these results relevant in potential future pandemic scenarios.

scholarly journals Effect of hydrogen peroxide solutions on growth of micromycetes Saprolegniaceae on egg membranes of beluga (Huso huso) embryos and on indicators of its embryonic development during incubation

2021 ◽  
Vol 0 (4) ◽  
pp. 115-125
Author(s):  
Victoria Barinova ◽  
Anna Aleksandrovna Bakhareva ◽  
Margarita Evgenevna Perunova ◽  
Ralina Rasimovna Tangatarova
Keyword(s):  
Hydrogen Peroxide ◽  
Embryonic Development ◽  
Negative Impact ◽  
Fish Farming ◽  
Biological Indicators ◽  
Common Disease ◽  
Huso Huso ◽  
Experimental Works ◽  
Approved Drugs ◽  
The Impact

The article describes the data of the experimental works on assessing the influence of hydrogen peroxide so-lutions (0.05, 0.10, 0.20, 0.30, 0.40%) in different concentrations on the growth of the micromycetes Saprolegniaceae on egg membranes of beluga embryos and its embryonic development. In conditions of increasing intensification of aquacultures there is an acute problem regarding to the shortage of approved drugs for treating a common disease in fish farming - saprolegniosis. This disease is caused by species of different genus, the most common of which are the genus Achlya and Saprolegnia belonging to the biological kingdom Chromista (Stramenopila), the Oomycota phylum, the Oomycetes class, the Saprolegniales order, the Saprolegniaceae family. The impact of experimental hydrogen peroxide solutions on the beluga embryonic development and infection with saprolegnial micromycetes was assessed by fish-breeding biological indicators: the number of inseminated oocytes, number of survived embryos and hatched prelarvae, number of embryos infected with the micromycetes Saprolegniaceae and abnormally developing fish. Histological analysis of embryos at different stages of development was also carried out. The experimental works were carried out under production conditions in an incubation apparatus of “Sturgeon” type. The experimental groups were formed in accordance with the experimental solutions’ concentrations. Upon the result of the studies it has been inferred that using a 0.05% hydrogen peroxide solution with a 10-minute-exposure proves effective. This solution sup-pressed growth of micromycetes Saprolegniaceae without any negative impact on the embryonic development. Other experimental solutions had a negative impact on beluga (Huso huso) embryonic development of in most cases, causing the increased mortality and, as a consequence, the increased infection rate.

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