ChemInform Abstract: Glutaraldehyde in Bio-Catalysts Design: A Useful Crosslinker and a Versatile Tool in Enzyme Immobilization

ChemInform ◽  
2014 ◽  
Vol 45 (21) ◽  
pp. no-no ◽  
Author(s):  
Oveimar Barbosa ◽  
Claudia Ortiz ◽  
Angel Berenguer-Murcia ◽  
Rodrigo Torres ◽  
Rafael C. Rodrigues ◽  
...  
Keyword(s):  
Enzyme Immobilization ◽  
Versatile Tool

scholarly journals Optimization of enzyme immobilization on magnetic microparticles using 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC) as a crosslinking agent

2015 ◽  
Vol 7 (24) ◽  
pp. 10291-10298 ◽  
Author(s):  
F. Kazenwadel ◽  
H. Wagner ◽  
B. E. Rapp ◽  
M. Franzreb
Keyword(s):  
Enzyme Activity ◽  
Enzyme Immobilization ◽  
Crosslinking Agent ◽  
Magnetic Microparticles ◽  
Versatile Tool

Enzyme immobilization is a versatile tool in biotransformation processes to enhance enzyme activity and to secure an easy separation of catalysts and products and the reusability of enzymes.

scholarly journals Glutaraldehyde in bio-catalysts design: a useful crosslinker and a versatile tool in enzyme immobilization

RSC Advances ◽  
2014 ◽  
Vol 4 (4) ◽  
pp. 1583-1600 ◽  
Author(s):  
Oveimar Barbosa ◽  
Claudia Ortiz ◽  
Ángel Berenguer-Murcia ◽  
Rodrigo Torres ◽  
Rafael C. Rodrigues ◽  
...  
Keyword(s):  
Enzyme Immobilization ◽  
Versatile Tool

scholarly journals Enzyme Immobilization in Polymerized Ionic Liquids-based Hydrogels for Active and Reusable Biocatalysts

SynOpen ◽  
2018 ◽  
Vol 02 (02) ◽  
pp. 0192-0199 ◽  
Author(s):  
A. Grollmisch ◽  
U. Kragl ◽  
J. Großeheilmann
Keyword(s):  
Ionic Liquids ◽  
Enzyme Immobilization ◽  
Kinetic Resolution ◽  
Enantiomeric Excess ◽  
Catalytic Activities ◽  
Nonpolar Solvents ◽  
Novozyme 435 ◽  
Versatile Tool ◽  
Full Conversion ◽  
Immobilization Method

Enzyme immobilization in polymerized ionic liquids (PILs) promises to be a versatile tool for simple recovery and reuse of catalysts. In this study, a raw extract of CalB was encapsulated in poly(VEImBr) and assessed with respect to solvent, temperature, amount of enzyme, leaching behavior, and reusability on the example of the kinetic resolution of rac-1-phenylethanol with vinyl acetate. This immobilization method increased the enzyme activity of the CalB raw extract in comparison to the non-immobilized enzyme. The desired product was synthesized with high enantiomeric excess (ee) and no leaching of active enzyme was observed in the experiments. The immobilization method was compared to Novozyme 435 and Lipozyme RM IM, as commercially available immobilisates. Nonpolar solvents, including n-heptane and n-dodecane, proved to be the best reaction solvents, showing nearly full conversion and high catalytic activities. The encapsulated ­lipase was easily recovered from the reaction mixture and reused for ten cycles.

Environmentally Friendly Preparation of Magnetic Composites Featuring Proteolytic Properties

INEOS OPEN ◽  
2020 ◽  
Author(s):  
N. A. Samoilova ◽  
Keyword(s):  
Enzyme Immobilization ◽  
Maleic Acid ◽  
Environmentally Friendly ◽  
Synthetic Polymer ◽  
Magnetic Composites ◽  
Interpolyelectrolyte Complex ◽  
Hydrolysis Rates ◽  
Poly Ethylene ◽  
Noncovalent Binding

The enzyme-containing magnetic composites are presented. The magnetic matrix for enzyme immobilization is obtained by sequential application of an amine-containing polysaccharide—chitosan and a synthetic polymer—poly(ethylene-alt-maleic acid) to the magnetite microparticles to form the interpolyelectrolyte complex shell. Then, the enzyme (trypsin) is immobilized by covalent or noncovalent binding. Thus, the suggested composites can be readily obtained in the environmentally friendly manner. The enzyme capacity of the resulting composites reaches 28.0–32.6 mg/g. The maximum hydrolysis rates of the H-Val-Leu-Lys-pNA substrate provided by these composites range within 0.60·10–7–0.77·10–7 M/min.

Biocatalytic Ketone Reductions Using Biobeads

2020 ◽  
Author(s):  
Jia Shen Chew ◽  
Ken Chi Lik Lee ◽  
THI THANH NHA HO
Keyword(s):  
Enzyme Immobilization ◽  
High Throughput ◽  
High Throughput Screening ◽  
Chiral Hplc ◽  
Micro Scale

<p>Lee and coworkers offers a kind of new concept to enzyme immobilization and explores its suitability in the context of miniaturisation and high-throughput screening. Here, polystyrene-immobilized ketoreductases are compared with its non-immobilized counterparts in terms of conversion and stereoselectivity (both determined by chiral HPLC), and the study indicates that the BioBeads perform similarly (sometimes slightly more selective) which may be useful whenever defined micro-scale amounts of biocatalysts were required in high-throughput experiment settings.</p>

CRUCIBLE S7

Alloy Digest ◽  
1996 ◽  
Vol 45 (7) ◽  
Keyword(s):  
Heat Treatment ◽  
Fracture Toughness ◽  
Physical Properties ◽  
Tool Steel ◽  
Cold Work ◽  
Heat Treating ◽  
High Shock ◽  
Unusual Combination ◽  
Versatile Tool ◽  
Shock Resistance

Abstract Crucible S7 is a chromium/molybdenum tool steel developed to produce the unusual combination of high shock resistance and toughness together with ease of machining and heat treatment. It is a versatile tool steel applicable for both hot and cold work shock applications. This datasheet provides information on composition, physical properties, hardness, and elasticity as well as fracture toughness. It also includes information on heat treating, machining, and joining. Filing Code: TS-543. Producer or source: Crucible Service Centers.

Plant Growth Promoting Bacteria (PGPB) - A Versatile Tool for Plant Health Management

2019 ◽  
Vol 1 (1) ◽  
pp. 1
Author(s):  
Salah Eddin Khabbaz ◽  
D. Ladhalakshmi ◽  
Merin Babu ◽  
A. Kandan ◽  
V. Ramamoorthy ◽  
...  
Keyword(s):  
Plant Growth ◽  
Health Management ◽  
Plant Health ◽  
Plant Growth Promoting Bacteria ◽  
Versatile Tool ◽  
Plant Growth Promoting ◽  
Growth Promoting
Sign in / Sign up

Export Citation Format

Share Document