The Effects of Teach Back Method on Caregivers’ Strain in Handling Patients with Prolonged Immobilization

Background: Caregivers’ strain mainly comes from lack of relevant care knowledge and nursing skills. Traditional health education is just a one-way information transmission mode without evaluation and feedback. Objective: To help caregivers’ memory, the researcher utilized a quasi-experimental design to measure the effectiveness of the teach-back method on caregivers’ strain in handling patients with prolonged immobilization. Method: A total of forty caregivers were averagely assigned into treatment (Teach-Back) and comparison group (traditional). Participant’s Data Sheet, The Zarit Burden Interview and Teach Back Assessment Tool was used to gather data. Results: There was a significant difference in caregivers’ strain before and after the Teach-Back Method in treatment (p<0.001) and comparison group (p <0.001). Likewise, a significant difference existed after the Teach-Back Method in the treatment group (p <0.001). Conclusion: The Teach-Back Method is an effective intervention in reducing caregivers’ strain in handling patients with prolonged immobilization.

Grouper Imotilization Technique Epinephelus sp. Using Datura Metel L. Seed Extract

This study is about fish anesthesia using natural anesthetic ingredients. Natural anesthetic ingredients aim to reduce metabolism in Epinephelus sp. grouper fish. The immobilization method can be used to minimize high metabolism in Epinephelus sp. grouper fish. The purpose of this study was to obtain the influence of Datura metel L. seed extract on the onset time, recovery time, and live graduation of grouper fish Epinephelus sp. The results of the study, using extracts of natural anesthetic ingredients Datura metel L.seed with concentrations of 25%, 30%, and 35% can be concluded that it has a different influence (P<0.05) on the onset and recovered grouper fish. This study gave a life graduation rate of 100% in each concentration. The best concentration in this study was 35% concentration, with the fastest onset time of 3 minutes with a recovery time of 10.67 minutes. This study used a non-Factorial Complete Randomized Design

Obtaining viable Azospirillum brasilense SR80 cells encapsulated in alginate hydrogel

Significant researches aimed at the greening of agro-industrial production are focused on obtaining immobilized bacterial preparations with preserved proliferative function and metabolic activity. Herein, we investigated the possibility of bacteria of the genus Azospirillum to be immobilized in Ca-alginate beads. A. brasilense SR80 cells, encapsulated in an alginate hydrogel, were obtained using the “soft” immobilization method based on physical binding. We demonstrated the retained respiratory activity and growth ability of the bacteria during immobilization, thus confirming the advantageous prospects of alginate templates for azospirilla encapsulation.

Conversion of waste cooking oil by rhodococcal lipase immobilized in gellan gum

Recently, the application of lipase enzyme as biocatalyst in the conversion of waste cooking oil (WCO) to free fatty acids and glycerol has been trending well. Therefore, the present study attempts to use WCO which is found in abundance in Malaysia as the substrate for halal microbial lipase conversion to glycerol which can be exploited in the food industry. The workability of free lipase for WCO conversion, however suffers severely due to potential denaturation of the enzyme and extended reaction time. Thus, this study embraced the immobilization method to encapsulate crude lipase extracted from Rhodococcus pyridinivorans strain UCC 0009 in gellan gum and calcium alginate, respectively and compared their ability for WCO conversion to free crude lipase. The gellan gum and calcium alginate-immobilized crude lipase evidently exhibited greater WCO conversion, demonstrating 2.18-fold and 1.61-fold enhanced lipase activity, respectively in comparison to free crude lipase. The repeated reuse of the gellan gum-immobilized crude lipase maintained reasonable lipase activity for 9 cycles, retaining an average 85 % WCO conversion for the first seven cycles and 67 % conversion in the subsequent batches. Thus, the immobilized halal lipase can be foreseen as a green substitute to chemical catalyst for WCO conversion which meets the worldwide demand for clean technologies.

AuPd Bimetallic Nanoparticles Supported on Reduced Graphene Oxide Nanosheets as Catalyst For Hydrogen Generation from Formic Acid under Ambient Temperature

Au, Pd and bimetallic AuxPdy nanoparticles (x/y mole ratio Au/Pd: 3:1, 1:1 and 1:3) supported on reduced graphene oxide (rGO) as catalysts were synthesised by sol-immobilization method. The structural characteristics,...

Immobilization of Pd (II)-containing N-heterocyclic carbene ligand on porous organic polymers: efficient and recyclable catalysts for Suzuki-Miyaura reactions

Palladium coordinated with N-heterocyclic carbene-functionalized porous organic polymer (Pd@POPs) was successfully prepared via Scholl coupling reaction and successive immobilization method. The protocol features simple reaction conditions, easy separation, high yield...

Inkjet Printing-Based Immobilization Method for a Single-Step and Homogeneous Competitive Immunoassay in Microchannel Arrays

In this study, we report an inkjet printing-based method for the immobilization of different reactive analytical reagents on a single microchannel for a single-step and homogeneous solution-based competitive immunoassay. The immunoassay microdevice is composed of a poly(dimethylsiloxane) microchannel that is patterned using inkjet printing by two types of reactive reagents as dissolvable spots, namely, antibody-immobilized graphene oxide and a fluorescently labeled antigen. Since nanoliter-sized droplets of the reagents could be accurately and position-selectively spotted on the microchannel, different reactive reagents were simultaneously immobilized onto the same microchannel, which was difficult to achieve in previously reported capillary-based single-step bioassay devices. In the present study, the positions of the reagent spots and amount of reagent matrix were investigated to demonstrate the stable and reproducible immobilization and a uniform dissolution. Finally, a preliminary application to a single-step immunoassay of C-reactive protein was demonstrated as a proof of concept.