Flow cytometry as a diagnostic tool in lymphomatous or leukemic meningitis

Cytological examination is a valuable diagnostic tool in case of a serous effusion. The firstmanifestation of malignancy may be an effusion of the pleural, pericardial, or peritoneal cavity, especially in carcinoma of the ovary, or lung, and malignant mesothelioma. In other malignancies effusions may occur in the course of the disease. The contribution by Motherby et al. in this issue of ACP focuses on the contribution of image and flow cytometry to establish the presence or absence of malignancy in serous effusions [16]. They point out that the sensitivity of DNA image cytometry in equivocal effusions may be as high as 87.5%, and that for the detection of malignancy, DNA image cytometry is superior to flow cytometry.

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Lymphocyte and monocyte flow cytometry immunophenotyping as a diagnostic tool in uncharacteristic inflammatory disorders

BMC Infectious Diseases ◽

10.1186/1471-2334-10-205 ◽

2010 ◽

Vol 10 (1) ◽

Cited By ~ 13

Author(s):

Helena Janols ◽

Anders Bredberg ◽

Irene Thuvesson ◽

Sabina Janciauskiene ◽

Olof Grip ◽

...

Keyword(s):

Flow Cytometry ◽

Diagnostic Tool ◽

Inflammatory Disorders

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Performance evaluation of anti-fixed Leishmania infantum promastigotes immunoglobulin G (IgG) detected by flow cytometry as a diagnostic tool for visceral Leishmaniasis

Journal of Immunological Methods ◽

10.1016/j.jim.2019.02.009 ◽

2019 ◽

Vol 469 ◽

pp. 18-25 ◽

Cited By ~ 2

Author(s):

Elis D. Silva ◽

Beatriz C. Oliveira ◽

Andresa P. Oliveira ◽

Wagner J.T. Santos ◽

George T. Diniz ◽

...

Keyword(s):

Flow Cytometry ◽

Performance Evaluation ◽

Visceral Leishmaniasis ◽

Immunoglobulin G ◽

Diagnostic Tool ◽

Leishmania Infantum

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Impact of Flow Cytometry in Liver Cytopathology

Acta Cytologica ◽

10.1159/000370042 ◽

2015 ◽

Vol 59 (1) ◽

pp. 51-60 ◽

Cited By ~ 1

Author(s):

Jennifer P. Bynum ◽

Amy Duffield ◽

Syed Z. Ali

Keyword(s):

Flow Cytometry ◽

Fine Needle Aspiration ◽

Diagnostic Tool ◽

Needle Aspiration ◽

Lymphoproliferative Disorders ◽

Johns Hopkins Hospital ◽

Lymphoproliferative Diseases ◽

Fine Needle ◽

Negative Results

Background: Fine needle aspiration (FNA) is commonly used as a diagnostic tool for the evaluation of lymphoproliferative diseases. Cytomorphology alone is often insufficient for the diagnosis and subclassification of lymphoma; therefore, flow cytometry (FC) plays an important role in the characterization of lymphoproliferative disorders. This study reviews our experience with FC on liver FNA at the Johns Hopkins Hospital. Methods: 2,424 liver FNAs performed over a 21-year period were reviewed for clinical FC data (n = 74) or a subsequent diagnosis of lymphoma in the liver without FC data (n = 40). Results: In our study, 114 cases (4.7%) were included out of the 2,424 liver FNAs performed during the study period. Lymphoma was diagnosed 79 times. Cytomorphology alone was diagnostic of lymphoma in 45 cases, and in 33 cases both the cytomorphology and the FC were consistent with a diagnosis of lymphoma. Neither FC nor cytomorphology were diagnostic of lymphoma on 1 specimen. In 39 cases, FC had negative results on a lesion suspicious for lymphoma based on cytomorphology. In several nonlymphoma cases, FC provided information that allowed further subclassification of the neoplasm. Conclusion: FC is a useful adjuvant diagnostic test for liver FNAs performed on patients with lymphoproliferative disorders.

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Flow Cytometry as a Diagnostic Tool for Hereditary Spherocytosis

Acta Haematologica ◽

10.1159/000094679 ◽

2006 ◽

Vol 116 (3) ◽

pp. 186-191 ◽

Cited By ~ 22

Author(s):

Gudrun Stoya ◽

Bernd Gruhn ◽

Heinz Vogelsang ◽

Eckehard Baumann ◽

Werner Linss

Keyword(s):

Flow Cytometry ◽

Diagnostic Tool ◽

Hereditary Spherocytosis

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Cerebrospinal Fluid Flow Cytometry in Acute Lymphoblastic Leukemia/Lymphoma: Is It Beneficial?.

Blood ◽

10.1182/blood.v120.21.2576.2576 ◽

2012 ◽

Vol 120 (21) ◽

pp. 2576-2576

Author(s):

Fuad El Rassi ◽

Zahi Mitri ◽

Leonard T Heffner ◽

Amelia Langston ◽

Edmund K. Waller ◽

...

Keyword(s):

Flow Cytometry ◽

Cerebrospinal Fluid ◽

Acute Lymphoblastic Leukemia ◽

Conflicts Of Interest ◽

Lymphoblastic Leukemia ◽

Additional Benefit ◽

Csf Flow ◽

Cerebrospinal Fluid Flow ◽

Systemic Relapse ◽

Leukemic Meningitis

Abstract Abstract 2576 Cerebrospinal fluid (CSF) involvement by leukemic blasts occurs in fewer than 10 % of adult patients with newly diagnosed acute lymphoblastic leukemia/lymphoma (ALL). Leukemic meningitis is diagnosed by microscopic detection of blasts in the CSF. Flow cytometry is a highly sensitive tool for detection of aberrant cells. We sought to analyze the additional benefit flow cytometry might provide for the diagnosis of leukemic meningitis. Between 11/2007 and 8/2011, 80 patients were diagnosed with ALL and treated at Emory University. 800 CSF samples were available for analysis 80 of which were collected from a diagnostic lumbar puncture (LP), 689 from follow-up LPs and 31 from LPs obtained at the time of relapse. As shown in the table, flow cytometry confirmed the presence of leukemic blasts in one, four and five samples diagnosed with leukemic meningitis by cytology at diagnosis, different stages of treatment and relapse, respectively. One and three samples were positive for leukemic blasts by cytology but negative by flow cytometry during different treatment stages and relapse respectively. We conclude that flow cytometry provided no additional benefit to cytology in the diagnosis of leukemic meningitis. Table: CSF Cytology and Flow Cytometry in 80 Adult ALL patients: CSF samples New Diagnosis N=80 Induction/Consolidation/Intensification/Maintenance/Remission/Post-transplant N = 689 Systemic relapse N = 31 N Cytology N = 80 Flow cytometry N = 66 Cytology N = 689 Flow cytometry N = 188 Cytology N = 31 Flow cytometry N = 13 Negative 79/80 65/66 684/689 184/188 23/31 8/13 Positive 1/80 1/66∼ 5/689 4/188∼* 8/31 5/13∼** ∼ CSF samples positive by flow cytometry were also positive by cytology * One CSF sample was positive by cytology but negative by flow cytometry ** CSF flow cytometry was not done in 3/8 positive CSF samples by cytology Disclosures: No relevant conflicts of interest to declare.

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