A High‐Performing Metal‐Free Photoactivatable Nitric Oxide Donor with a Green Fluorescent Reporter

ChemPhotoChem ◽  
2020 ◽  
Author(s):  
Cristina Parisi ◽  
Mimimorena Seggio ◽  
Aurore Fraix ◽  
Salvatore Sortino
Keyword(s):  
Nitric Oxide ◽  
Nitric Oxide Donor ◽  
Fluorescent Reporter ◽  
High Performing ◽  
Metal Free ◽  
Green Fluorescent

Enhanced gastroprotective and anti-ulcerogenic activities in rats of a new class of proton pump inhibitor containing nitrosothiol nitric oxide donor

2001 ◽  
Vol 120 (5) ◽  
pp. A144-A145
Author(s):  
J SAHA ◽  
T WANG ◽  
R STEWART ◽  
M TROCHA ◽  
M SHUMWAY ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Proton Pump Inhibitor ◽  
Proton Pump ◽  
Nitric Oxide Donor ◽  
New Class

Effects of the Nitric Oxide Donor Sodium Nitroprusside on Intracellular pH and Contraction in Hypertrophied Myocytes

Circulation ◽  
1997 ◽  
Vol 95 (9) ◽  
pp. 2303-2311 ◽  
Author(s):  
Nobuhiko Ito ◽  
Josef Bartunek ◽  
Kenneth W. Spitzer ◽  
Beverly H. Lorell
Keyword(s):  
Nitric Oxide ◽  
Sodium Nitroprusside ◽  
Intracellular Ph ◽  
Nitric Oxide Donor

scholarly journals Cephalosporin nitric oxide-donor prodrug DEA-C3D disperses biofilms formed by clinical cystic fibrosis isolates of Pseudomonas aeruginosa

2019 ◽  
Vol 75 (1) ◽  
pp. 117-125 ◽  
Author(s):  
Odel Soren ◽  
Ardeshir Rineh ◽  
Diogo G Silva ◽  
Yuming Cai ◽  
Robert P Howlin ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Cystic Fibrosis ◽  
Pseudomonas Aeruginosa ◽  
Laser Scanning ◽  
Laser Scanning Microscopy ◽  
Clinical Isolates ◽  
Nitric Oxide Donor ◽  
Confocal Laser ◽  
Broth Microdilution Method

Abstract Objectives The cephalosporin nitric oxide (NO)-donor prodrug DEA-C3D (‘DiEthylAmin-Cephalosporin-3′-Diazeniumdiolate’) has been shown to initiate the dispersal of biofilms formed by the Pseudomonas aeruginosa laboratory strain PAO1. In this study, we investigated whether DEA-C3D disperses biofilms formed by clinical cystic fibrosis (CF) isolates of P. aeruginosa and its effect in combination with two antipseudomonal antibiotics, tobramycin and colistin, in vitro. Methods β-Lactamase-triggered release of NO from DEA-C3D was confirmed using a gas-phase chemiluminescence detector. MICs for P. aeruginosa clinical isolates were determined using the broth microdilution method. A crystal violet staining technique and confocal laser scanning microscopy were used to evaluate the effects of DEA-C3D on P. aeruginosa biofilms alone and in combination with tobramycin and colistin. Results DEA-C3D was confirmed to selectively release NO in response to contact with bacterial β-lactamase. Despite lacking direct, cephalosporin/β-lactam-based antibacterial activity, DEA-C3D was able to disperse biofilms formed by three P. aeruginosa clinical isolates. Confocal microscopy revealed that DEA-C3D in combination with tobramycin produces similar reductions in biofilm to DEA-C3D alone, whereas the combination with colistin causes near complete eradication of P. aeruginosa biofilms in vitro. Conclusions DEA-C3D is effective in dispersing biofilms formed by multiple clinical isolates of P. aeruginosa and could hold promise as a new adjunctive therapy to patients with CF.

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