Smooth muscle actin immunostain is useful for the diagnosis of Trichomonas vaginalis in vaginal smears

2021 ◽  
Author(s):  
Ni Fang ◽  
Deng Tang ◽  
Hanjie Zhang ◽  
Jie Zeng
Keyword(s):  
Smooth Muscle ◽  
Trichomonas Vaginalis ◽  
Smooth Muscle Actin ◽  
Muscle Actin ◽  
Vaginal Smears

scholarly journals Cytotoxic Influence of Khat (Catha edulis (Vahl) Forssk. ex Endl) on Oral Fibroblasts, Squamous Carcinoma Cells, and Expression of α Smooth Muscle Actin

2021 ◽  
Vol 11 (8) ◽  
pp. 3524
Author(s):  
Azeem Ul Yaqin Syed ◽  
Muhammad A. Ahmed ◽  
Eman I. AlSagob ◽  
Mansour Al-Askar ◽  
Abdulrahman M. AlMubarak ◽  
...  
Keyword(s):  
Cell Death ◽  
Smooth Muscle ◽  
Smooth Muscle Actin ◽  
Control Cell ◽  
Squamous Carcinoma ◽  
Carcinoma Cells ◽  
Muscle Actin ◽  
Catha Edulis ◽  
Squamous Carcinoma Cells ◽  
Dose Dependent

The aim was to determine the cytotoxicity of Khat (Catha edulis (Vahl) Forssk. ex Endl) on normal oral fibroblasts (NOFs) and SCC4 (squamous carcinoma cells) along with expression of α-smooth muscle actin (α-SMA) in fibroblasts. Khat filtrate was prepared to obtain a concentrated viscous solution. NOFs and SCC4 cells were cultured in biological cabinets and were grown in Dulbeccos’ modified Eagles medium. Frozen cells were thawed at 37 °C and cell seeding was performed. NOFs and SCC4 cells were seeded on 96 well plates and allowed to attach. The medium was removed and a fresh medium containing different concentrations of Khat was added. The group without Khat served as a negative control and 4% paraformaldehyde as the positive control. Cell viability was assessed using the MTT assay and effect of Khat on fibroblast and SCC4 phenotypes was evaluated by immunostaining. Analysis of variance was used to assess data (p < 0.05). NOF 316 showed cell death in response to 4% paraformaldehyde, 12.5, 6.25, and 3.12 mg/mL of Khat. The highest concentration of Khat (25 mg/mL) failed to cause cytotoxicity of NOF 316. NOF 319 and NOF 26 displayed cell death at all concentrations of Khat, however, cytotoxicity was not dose dependent. NOF 18 and SCC4 cells showed dose-dependent cell death. NOF 316 showed α-SMA expression after 1 mg/mL of Khat exposure. Not all fibroblasts were α-SMA-positive, suggesting specific activation of a subset of fibroblasts. Khat is cytotoxic to NOF and SCC4 cells. Furthermore, it can also cause activation and phenotypic changes in oral fibroblasts, indicating a potential role in progression of oral squamous cell carcinoma.

Antisense oligodeoxynucleotide complementary to smooth muscle ?-actin inhibits endothelial-mesenchymal transformation during chick cardiogenesis

1999 ◽  
Vol 216 (4/5) ◽  
pp. 489-498 ◽  
Author(s):  
Yuji Nakajima ◽  
Toshiyuki Yamagishi ◽  
Kazunori Yoshimura ◽  
Masahiko Nomura ◽  
Hiroaki Nakamura
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Actin ◽  
Antisense Oligodeoxynucleotide ◽  
Muscle Actin ◽  
Mesenchymal Transformation

Expression of alpha-smooth muscle actin, TGF-β1 and TGF-β type II receptor during connective tissue contraction

1997 ◽  
Vol 33 (8) ◽  
pp. 622-627 ◽  
Author(s):  
M. Reza Ghassemifar ◽  
Roy W. Tarnuzzer ◽  
Nasser Chegini ◽  
Erkki Tarpila ◽  
Gregory S. Schultz ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Connective Tissue ◽  
Smooth Muscle Actin ◽  
Type Ii ◽  
Muscle Actin ◽  
Alpha Smooth Muscle Actin ◽  
Tissue Contraction ◽  
Tgf Β1

The inhibitory effect of PLGA-encapsulated berberine on hepatotoxicity and α-smooth muscle actin (α-SMA) gene expression

Life Sciences ◽  
2021 ◽  
pp. 119884
Author(s):  
Asma Ahrari ◽  
Hossein Najafzadehvarzi ◽  
Ali Taravati ◽  
Fatemeh Tohidi
Keyword(s):  
Gene Expression ◽  
Smooth Muscle ◽  
Smooth Muscle Actin ◽  
Inhibitory Effect ◽  
Muscle Actin
Sign in / Sign up

Export Citation Format

Share Document