Genotoxic stress and activation of novel DNA repair enzymes in human endothelial cells and in the retinas and kidneys of streptozotocin diabetic rats

2012 ◽  
Vol 28 (4) ◽  
pp. 329-337 ◽  
Author(s):  
Chunyan Wang ◽  
Biju George ◽  
Shali Chen ◽  
Biao Feng ◽  
Xiaokun Li ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Dna Repair ◽  
Genotoxic Stress ◽  
Diabetic Rats ◽  
Human Endothelial Cells ◽  
Dna Repair Enzymes ◽  
Repair Enzymes ◽  
Streptozotocin Diabetic Rats

scholarly journals DNA damage shifts circadian clock time via Hausp-dependent Cry1 stabilization

eLife ◽  
2015 ◽  
Vol 4 ◽  
Author(s):  
Stephanie J Papp ◽  
Anne-Laure Huber ◽  
Sabine D Jordan ◽  
Anna Kriebs ◽  
Madelena Nguyen ◽  
...  
Keyword(s):  
Dna Damage ◽  
Dna Repair ◽  
Circadian Clock ◽  
Transcriptional Response ◽  
Genotoxic Stress ◽  
Genomic Integrity ◽  
Clock Time ◽  
Dna Repair Enzymes ◽  
Repair Enzymes ◽  
Specific Protease

The circadian transcriptional repressors cryptochrome 1 (Cry1) and 2 (Cry2) evolved from photolyases, bacterial light-activated DNA repair enzymes. In this study, we report that while they have lost DNA repair activity, Cry1/2 adapted to protect genomic integrity by responding to DNA damage through posttranslational modification and coordinating the downstream transcriptional response. We demonstrate that genotoxic stress stimulates Cry1 phosphorylation and its deubiquitination by Herpes virus associated ubiquitin-specific protease (Hausp, a.k.a Usp7), stabilizing Cry1 and shifting circadian clock time. DNA damage also increases Cry2 interaction with Fbxl3, destabilizing Cry2. Thus, genotoxic stress increases the Cry1/Cry2 ratio, suggesting distinct functions for Cry1 and Cry2 following DNA damage. Indeed, the transcriptional response to genotoxic stress is enhanced in Cry1−/− and blunted in Cry2−/− cells. Furthermore, Cry2−/− cells accumulate damaged DNA. These results suggest that Cry1 and Cry2, which evolved from DNA repair enzymes, protect genomic integrity via coordinated transcriptional regulation.

scholarly journals DASH-type cryptochromes – solved and open questions

2020 ◽  
Vol 401 (12) ◽  
pp. 1487-1493
Author(s):  
Stephan Kiontke ◽  
Tanja Göbel ◽  
Annika Brych ◽  
Alfred Batschauer
Keyword(s):  
Dna Repair ◽  
Circadian Clock ◽  
Blue Light ◽  
Dna Repair Enzymes ◽  
Repair Enzymes ◽  
Open Questions ◽  
Essential Components ◽  
Repair Activity ◽  
Almost All ◽  
Uv Lesions

AbstractDrosophila, Arabidopsis, Synechocystis, human (DASH)-type cryptochromes (cry-DASHs) form one subclade of the cryptochrome/photolyase family (CPF). CPF members are flavoproteins that act as DNA-repair enzymes (DNA-photolyases), or as ultraviolet(UV)-A/blue light photoreceptors (cryptochromes). In mammals, cryptochromes are essential components of the circadian clock feed-back loop. Cry-DASHs are present in almost all major taxa and were initially considered as photoreceptors. Later studies demonstrated DNA-repair activity that was, however, restricted to UV-lesions in single-stranded DNA. Very recent studies, particularly on microbial organisms, substantiated photoreceptor functions of cry-DASHs suggesting that they could be transitions between photolyases and cryptochromes.

p21Cip1/WAF1/Sdi1Does Not Affect Expression of Base Excision DNA Repair Enzymes During Chronic Oxidative Stress

2005 ◽  
Vol 7 (5-6) ◽  
pp. 719-725 ◽  
Author(s):  
Michael A. O'Reilly ◽  
Peter F. Vitiello ◽  
Sean C. Gehen ◽  
Rhonda J. Staversky
Keyword(s):  
Oxidative Stress ◽  
Dna Repair ◽  
Dna Repair Enzymes ◽  
Repair Enzymes ◽  
Chronic Oxidative Stress ◽  
Affect Expression ◽  
Base Excision ◽  
Base Excision Dna Repair

Genotype Profiles of Loci Encoding DNA Repair Enzymes in Newborn and Elderly Populations: No Evidence of Association with Longevity

2006 ◽  
Vol 7 (1) ◽  
pp. 35-41 ◽  
Author(s):  
C. S. Wilding ◽  
G. S. Rees ◽  
C. L. Relton ◽  
E. J. Tawn
Keyword(s):  
Dna Repair ◽  
Dna Repair Enzymes ◽  
Repair Enzymes ◽  
Elderly Populations

DNA Repair Enzymes in Mammalian Cells

1977 ◽  
pp. 263-322 ◽  
Author(s):  
Errol C. Friedberg ◽  
Kern H. Cook ◽  
James Duncan ◽  
Kristien Mortelmans
Keyword(s):  
Dna Repair ◽  
Mammalian Cells ◽  
Dna Repair Enzymes ◽  
Repair Enzymes

scholarly journals Structural and cellular analyses of cancer-associated mutations in DNA repair enzymes

2019 ◽  
Vol 75 (a1) ◽  
pp. a327-a327
Author(s):  
Brian E. Eckenroth ◽  
Ash Prakash ◽  
Brittany L. Carroll ◽  
Joann B. Sweasy ◽  
Sylvie Doublié
Keyword(s):  
Dna Repair ◽  
Dna Repair Enzymes ◽  
Repair Enzymes

scholarly journals Loss of expression of DNA repair enzymes MGMT, hMLH1, and hMSH2 during tumor progression in gastric cancer

2003 ◽  
Vol 6 (2) ◽  
pp. 86-95 ◽  
Author(s):  
Yoshihiko Kitajima ◽  
Kohji Miyazaki ◽  
Shiroh Matsukura ◽  
Masayuki Tanaka ◽  
Mutsuo Sekiguchi
Keyword(s):  
Gastric Cancer ◽  
Dna Repair ◽  
Tumor Progression ◽  
Dna Repair Enzymes ◽  
Repair Enzymes

Chapter 2. Detection of Oxidised DNA Using DNA Repair Enzymes

2009 ◽  
pp. 57-78 ◽  
Author(s):  
Amaya Azqueta ◽  
Sergey Shaposhnikov ◽  
Andrew R. Collins
Keyword(s):  
Dna Repair ◽  
Dna Repair Enzymes ◽  
Repair Enzymes
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