An innovative derivatization‐free IC‐MS/MS method for the detection of bisphosphonates in horse plasma

2020 ◽  
Vol 12 (10) ◽  
pp. 1452-1461
Author(s):  
Patrice Garcia ◽  
Justine Pinètre ◽  
Sylvain Morel ◽  
Murielle Jaubert ◽  
Xavier Deruy ◽  
...  
Keyword(s):  
Horse Plasma

Total weak acid concentration and effective dissociation constant of nonvolatile buffers in human plasma

2001 ◽  
Vol 91 (3) ◽  
pp. 1364-1371 ◽  
Author(s):  
Peter D. Constable
Keyword(s):  
Dissociation Constant ◽  
Human Plasma ◽  
Total Concentration ◽  
Weak Acid ◽  
Strong Ion Difference ◽  
Acid Base ◽  
Horse Plasma ◽  
Using Data ◽  
Species Specific

The strong ion approach provides a quantitative physicochemical method for describing the mechanism for an acid-base disturbance. The approach requires species-specific values for the total concentration of plasma nonvolatile buffers (Atot) and the effective dissociation constant for plasma nonvolatile buffers ( K a), but these values have not been determined for human plasma. Accordingly, the purpose of this study was to calculate accurate Atot and K a values using data obtained from in vitro strong ion titration and CO2tonometry. The calculated values for Atot (24.1 mmol/l) and K a (1.05 × 10−7) were significantly ( P < 0.05) different from the experimentally determined values for horse plasma and differed from the empirically assumed values for human plasma (Atot = 19.0 meq/l and K a = 3.0 × 10−7). The derivatives of pH with respect to the three independent variables [strong ion difference (SID), Pco 2, and Atot] of the strong ion approach were calculated as follows: [Formula: see text] [Formula: see text], [Formula: see text]where S is solubility of CO2 in plasma. The derivatives provide a useful method for calculating the effect of independent changes in SID+, Pco 2, and Atot on plasma pH. The calculated values for Atot and K a should facilitate application of the strong ion approach to acid-base disturbances in humans.

Application of the restricted-access precolumn packing material alkyl-diol silica in a column-switching system for the determination of ketoprofen enantiomers in horse plasma

2000 ◽  
Vol 871 (1-2) ◽  
pp. 153-161 ◽  
Author(s):  
W.R.G Baeyens ◽  
G Van der Weken ◽  
J Haustraete ◽  
H.Y Aboul-Enein ◽  
S Corveleyn ◽  
...  
Keyword(s):  
Column Switching ◽  
Packing Material ◽  
Switching System ◽  
Restricted Access ◽  
Horse Plasma ◽  
Diol Silica

A simplified strong ion model for acid-base equilibria: application to horse plasma

1997 ◽  
Vol 83 (1) ◽  
pp. 297-311 ◽  
Author(s):  
Peter D. Constable
Keyword(s):  
Human Plasma ◽  
Practical Method ◽  
Published Data ◽  
Strong Ion Difference ◽  
Weak Acids ◽  
Acid Base ◽  
Plasma Protein Concentration ◽  
Horse Plasma ◽  
Hasselbalch Equation

Constable, Peter D. A simplified strong ion model for acid-base equilibria: application to horse plasma. J. Appl. Physiol. 83(1): 297–311, 1997.—The Henderson-Hasselbalch equation and Stewart’s strong ion model are currently used to describe mammalian acid-base equilibria. Anomalies exist when the Henderson-Hasselbalch equation is applied to plasma, whereas the strong ion model does not provide a practical method for determining the total plasma concentration of nonvolatile weak acids ([Atot]) and the effective dissociation constant for plasma weak acids ( K a). A simplified strong ion model, which was developed from the assumption that plasma ions act as strong ions, volatile buffer ions ([Formula: see text]), or nonvolatile buffer ions, indicates that plasma pH is determined by five independent variables:[Formula: see text], strong ion difference, concentration of individual nonvolatile plasma buffers (albumin, globulin, and phosphate), ionic strength, and temperature. The simplified strong ion model conveys on a fundamental level the mechanism for change in acid-base status, explains many of the anomalies when the Henderson-Hasselbalch equation is applied to plasma, is conceptually and algebraically simpler than Stewart’s strong ion model, and provides a practical in vitro method for determining [Atot] and K a of plasma. Application of the simplified strong ion model to CO2-tonometered horse plasma produced values for [Atot] (15.0 ± 3.1 meq/l) and K a(2.22 ± 0.32 × 10−7 eq/l) that were significantly different from the values commonly assumed for human plasma ([Atot] = 20.0 meq/l, K a = 3.0 × 10−7 eq/l). Moreover, application of the experimentally determined values for [Atot] and K a to published data for the horse (known [Formula: see text], strong ion difference, and plasma protein concentration) predicted plasma pH more accurately than the values for [Atot] and K a commonly assumed for human plasma. Species-specific values for [Atot] and K a should be experimentally determined when the simplified strong ion model (or strong ion model) is used to describe acid-base equilibria.

Effects of endurance racing on horse plasma extracellular particle miRNA

2020 ◽  
Author(s):  
Getúlio P. Oliveira ◽  
William F. Porto ◽  
Cintia C. Palu ◽  
Lydyane M. Pereira ◽  
Alessandra M. M. Reis ◽  
...  
Keyword(s):  
Horse Plasma

Thin-layer chromatographic screening procedure for some drugs in horse plasma

1989 ◽  
Vol 496 ◽  
pp. 407-415 ◽  
Author(s):  
Tsuneo Tanaka ◽  
Sugako Aramaki ◽  
Atsushi Momose
Keyword(s):  
Thin Layer ◽  
Screening Procedure ◽  
Horse Plasma

The Influence of Training and Simulated Race on Horse Plasma Serotonin Levels

2020 ◽  
Vol 84 ◽  
pp. 102818 ◽  
Author(s):  
Pietro Medica ◽  
Renato Paolo Giunta ◽  
Giuseppe Bruschetta ◽  
Alida Maria Ferlazzo
Keyword(s):  
Horse Plasma ◽  
Plasma Serotonin
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