scholarly journals Development and validation of an analytical method for the simultaneous determination of the alcohol biomarkers ethyl glucuronide, ethyl sulfate, N‐acetyltaurine, and 16:0/18:1‐phosphatidylethanol in human blood

2021 ◽  
Author(s):  
Vanessa Hofmann ◽  
Tom R. Sundermann ◽  
Georg Schmitt ◽  
Marc Bartel
Keyword(s):  
Simultaneous Determination ◽  
Human Blood ◽  
Analytical Method ◽  
Ethyl Glucuronide ◽  
Ethyl Sulfate ◽  
Development And Validation

scholarly journals Development and Validation of a New HPLC-UV Method for the Simultaneous Determination of Triclabendazole and Ivermectin B1a in a Pharmaceutical Formulation

2010 ◽  
Vol 93 (6) ◽  
pp. 1868-1873 ◽  
Author(s):  
Maher Shurbaji ◽  
Mohamad H Abu Al Rub ◽  
Munib M Saket ◽  
Ali M Qaisi ◽  
Maher L Salim ◽  
...  
Keyword(s):  
Simultaneous Determination ◽  
Analytical Method ◽  
Significant Variation ◽  
Rp Hplc ◽  
System Suitability ◽  
Experimental Parameters ◽  
Development And Validation ◽  
Interday Precision ◽  
Peak Purity

Abstract A rapid, simple, and sensitive RP-HPLC analytical method was developed for the simultaneous determination of triclabendazole and ivermectin in combination using a C18 RP column. The mobile phase was acetonitrilemethanolwateracetic acid (56 36 7.5 0.5, v/v/v/v) at a pH of 4.35 and flow rate of 1.0 mL/min. A 245 nm UV detection wavelength was used. Complete validation, including linearity, accuracy, recovery, LOD, LOQ, precision, robustness, stability, and peak purity, was performed. The calibration curve was linear over the range 50.09150.26 g/mL for triclabendazole with r = 0.9999 and 27.0181.02 g/mL for ivermectin with r = 0.9999. Calculated LOD and LOQ for triclabendazole were 0.03 and 0.08 g/mL, respectively, and for ivermectin 0.07 and 0.20 g/mL, respectively. The intraday precision obtained was 98.71 with RSD of 0.87 for triclabendazole and 100.79 with RSD 0.73 for ivermectin. The interday precision obtained was 99.51 with RSD of 0.35 for triclabendazole and 100.55 with RSD of 0.59 for ivermectin. Robustness was also studied, and there was no significant variation of the system suitability of the analytical method with small changes in experimental parameters.

Analytical Method Development and Validation for Telmisartan, Chlorthalidone and Amlodipine by UV- Spectroscopic Method.

2021 ◽  
pp. 6049-6054
Author(s):  
Rutuja M. Sanap ◽  
Sarika R. Wavhale ◽  
Vaibhavi V. Kunjir ◽  
Rajkumar V. Shete
Keyword(s):  
Simultaneous Determination ◽  
Analytical Method ◽  
Dosage Form ◽  
Method Development ◽  
Correction Method ◽  
Absorption Correction ◽  
Method Development And Validation ◽  
Development And Validation ◽  
Tablet Dosage

A simple, sensitive and accurate UV- spectrophotometric absorption correction method has been developed for simultaneous determination of Telmisartan, Amlodipine and Chlorthalidone in combined tablet dosage form. Analytical method development and validation plays important role in the discovery manufacture of pharmaceuticals and development. In this paper, absorption correction method is used for multi-component analysis. The wavelengths selected for the analysis were 311nm for Telmisartan, 228nm for Chlorthalidone and 253nm for Amlodipine. Beer’s law obeyed the concentration range of 2-10 µg /ml, 2-10 µg /ml and 5-25 µg/ ml for Telmisartan, Amlodipine and Chlorthalidone. Methanol is used as a solvent. The accuracy of the method was assessed by recovery studies and was found between the range of 100% to 110% for Telmisartan, 85% to 110% for Amlodipine and 85% to 105% Chlorthalidone. The % RSD value was found to be less than 2. Thus, the method was simple, precise, economic, rapid, accurate and can be successfully applied for simultaneous determination of Telmisartan, Amlodipine and Chlorthalidone in combined tablet dosage form.

scholarly journals Analytical Method Development and validation for Simultaneous Determination of Atenolol and Nitrendipine in Pharmaceutical Dosage Form by RP-HPLC Method

2016 ◽  
Vol 03 (01) ◽  
pp. 14-22
Author(s):  
Prathap Boobalan ◽  
Srinivasa Rao Gorantla ◽  
Gobalakrishnan Pandurangan ◽  
Venkata Swathikrishna Kavuluru ◽  
Priyanka Vadthey
Keyword(s):  
Simultaneous Determination ◽  
Analytical Method ◽  
Dosage Form ◽  
Method Development ◽  
Hplc Method ◽  
Pharmaceutical Dosage Form ◽  
Rp Hplc ◽  
Method Development And Validation ◽  
Development And Validation

scholarly journals Development and validation of a simple high-performance liquid chromatography analytical method for simultaneous determination of phytosterols, cholesterol and squalene in parenteral lipid emulsions

2017 ◽  
Vol 32 (2) ◽  
pp. e4084 ◽  
Author(s):  
Ana Novak ◽  
Mercè Gutiérrez-Zamora ◽  
Lluís Domenech ◽  
Josep M. Suñé-Negre ◽  
Montserrat Miñarro ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Simultaneous Determination ◽  
Analytical Method ◽  
High Performance ◽  
Lipid Emulsions ◽  
Development And Validation

Simultaneous determination of twelve dyes in meat products: Development and validation of an analytical method based on HPLC-UV-diode array detection

2019 ◽  
Vol 285 ◽  
pp. 1-9 ◽  
Author(s):  
Marco Iammarino ◽  
Annalisa Mentana ◽  
Diego Centonze ◽  
Carmen Palermo ◽  
Michele Mangiacotti ◽  
...  
Keyword(s):  
Simultaneous Determination ◽  
Analytical Method ◽  
Meat Products ◽  
Diode Array ◽  
Diode Array Detection ◽  
Array Detection ◽  
Development And Validation
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