Lable-Free Electrochemical DNA Sensor Based on Gold Nanoparticles/Poly(neutral red) Modified Electrode

Voltammetric DNA sensor has been proposed on the platform of glassy carbon electrode covered with carbon black with adsorbed pillar[5]arene molecules. Electropolymerization of Neutral Red performed in the presence of native or oxidatively damaged DNA resulted in formation of hybrid material which activity depended on the DNA conditions. The assembling of the surface layer was confirmed by scanning electron microscopy and electrochemical impedance spectroscopy. The influence of DNA and pillar[5]arene on redox activity of polymeric dye was investigated and a significant increase of the peak currents was found for DNA damaged by reactive oxygen species generated by Cu2+/H2O2 mixture. Pillar[5]arene improves the electron exchange conditions and increases the response and its reproducibility. The applicability of the DNA sensor developed was shown on the example of ascorbic acid as antioxidant. It decreases the current in the concentration range from 1.0 μM to 1.0 mM. The possibility to detect antioxidant activity was qualitatively confirmed by testing tera infusion. The DNA sensor developed can find application in testing of carcinogenic species and searching for new antitumor drugs.

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Electrochemical DNA Sensor Based on Chitosan-Graphene and Electrodeposited Gold Nanoparticle Modified Electrode for the Detection of Staphylococcus aureus nuc Gene Sequence

Current Analytical Chemistry ◽

10.2174/1573411013666170607123122 ◽

2018 ◽

Vol 14 (2) ◽

pp. 159-165 ◽

Cited By ~ 3

Author(s):

Xueliang Niu ◽

Wen Zheng ◽

Xiaoyan Li ◽

Wenshu Zhao ◽

Zuorui Wen ◽

...

Keyword(s):

Staphylococcus Aureus ◽

Gold Nanoparticle ◽

Modified Electrode ◽

Gene Sequence ◽

Dna Sensor ◽

Electrochemical Dna Sensor

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Electrochemical DNA Sensor for hly Gene of Listeria Monocytogenes by Three-Dimensional Graphene and Gold Nanocomposite Modified Electrode

International Journal of Electrochemical Science ◽

10.20964/2017.05.04 ◽

2017 ◽

pp. 4086-4095 ◽

Cited By ~ 4

Author(s):

Lijun Yan ◽

Keyword(s):

Listeria Monocytogenes ◽

Modified Electrode ◽

Three Dimensional ◽

Dna Sensor ◽

Electrochemical Dna Sensor

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Electrochemical DNA sensor for Staphylococcus aureus nuc gene sequence with zirconia and graphene modified electrode

Journal of Solid State Electrochemistry ◽

10.1007/s10008-015-2893-9 ◽

2015 ◽

Vol 19 (8) ◽

pp. 2431-2438 ◽

Cited By ~ 9

Author(s):

Wei Sun ◽

Xiuli Wang ◽

Wencheng Wang ◽

Yongxi Lu ◽

Jingwen Xi ◽

...

Keyword(s):

Staphylococcus Aureus ◽

Modified Electrode ◽

Gene Sequence ◽

Dna Sensor ◽

Electrochemical Dna Sensor

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A label-free electrochemical DNA sensor based on ZrO2/poly(thionine)/CNT modified electrode and its application for detecting CaMV35S transgene gene sequence

Analytical Methods ◽

10.1039/c5ay00384a ◽

2015 ◽

Vol 7 (7) ◽

pp. 3164-3168 ◽

Cited By ~ 9

Author(s):

Na Zhang ◽

Keying Zhang ◽

Li Zhang ◽

Hongyan Wang ◽

Hongwei Shi ◽

...

Keyword(s):

Modified Electrode ◽

Gene Sequence ◽

Dna Sensor ◽

Label Free ◽

Schematic Representation ◽

Electrochemical Dna Sensor ◽

Hybridization Detection

Schematic representation of the immobilization of p-ssDNA and hybridization detection.

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Electrochemical DNA Sensor for Sensitive BRCA1 Detection Based on DNA Tetrahedral-Structured Probe and Poly-Adenine Mediated Gold Nanoparticles

Biosensors ◽

10.3390/bios10070078 ◽

2020 ◽

Vol 10 (7) ◽

pp. 78

Author(s):

Dezhi Feng ◽

Jing Su ◽

Guifang He ◽

Yi Xu ◽

Chenguang Wang ◽

...

Keyword(s):

Breast Cancer ◽

Gold Nanoparticles ◽

Early Diagnosis ◽

Limit Of Detection ◽

High Sensitivity ◽

Dna Sensor ◽

Electrochemical Dna Sensor ◽

Target Dna ◽

Potential Applications ◽

Sandwich System

BRCA1 is the biomarker for the early diagnosis of breast cancer. Detection of BRCA1 has great significance for the genetic analysis, early diagnosis and clinical treatment of breast cancer. In this work, we developed a simple electrochemical DNA sensor based on a DNA tetrahedral-structured probe (TSP) and poly-adenine (polyA) mediated gold nanoparticles (AuNPs) for the sensitive detection of BRCA1. A thiol-modified TSP was used as the scaffold on the surface of the screen-printed AuNPs electrode. The capture DNA (TSP) and reporter DNA were hybridized to the target DNA (BRCA1), respectively, to form the typical sandwich system. The nanocomposites of reporter DNA (polyA at the 5′ end) combined with AuNPs were employed for signal amplification which can capture multiple enzymes by the specificity between biotin and streptavidin. Measurements were completed in the electrochemical workstation by cyclic voltammetry and amperometry and we obtained the low limit of detection of 0.1 fM with the linear range from 1 fM to 1 nM. High sensitivity and good specificity of the proposed electrochemical DNA sensor showed potential applications in clinical early diagnosis for breast cancer.

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