scholarly journals Electrochemical DNA Sensor for Sensitive BRCA1 Detection Based on DNA Tetrahedral-Structured Probe and Poly-Adenine Mediated Gold Nanoparticles

Biosensors ◽  
2020 ◽  
Vol 10 (7) ◽  
pp. 78
Author(s):  
Dezhi Feng ◽  
Jing Su ◽  
Guifang He ◽  
Yi Xu ◽  
Chenguang Wang ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Gold Nanoparticles ◽  
Early Diagnosis ◽  
Limit Of Detection ◽  
High Sensitivity ◽  
Dna Sensor ◽  
Electrochemical Dna Sensor ◽  
Target Dna ◽  
Potential Applications ◽  
Sandwich System

BRCA1 is the biomarker for the early diagnosis of breast cancer. Detection of BRCA1 has great significance for the genetic analysis, early diagnosis and clinical treatment of breast cancer. In this work, we developed a simple electrochemical DNA sensor based on a DNA tetrahedral-structured probe (TSP) and poly-adenine (polyA) mediated gold nanoparticles (AuNPs) for the sensitive detection of BRCA1. A thiol-modified TSP was used as the scaffold on the surface of the screen-printed AuNPs electrode. The capture DNA (TSP) and reporter DNA were hybridized to the target DNA (BRCA1), respectively, to form the typical sandwich system. The nanocomposites of reporter DNA (polyA at the 5′ end) combined with AuNPs were employed for signal amplification which can capture multiple enzymes by the specificity between biotin and streptavidin. Measurements were completed in the electrochemical workstation by cyclic voltammetry and amperometry and we obtained the low limit of detection of 0.1 fM with the linear range from 1 fM to 1 nM. High sensitivity and good specificity of the proposed electrochemical DNA sensor showed potential applications in clinical early diagnosis for breast cancer.

scholarly journals Quantification of DNA by a Thermal-Durable Biosensor Modified with Conductive Poly(3,4-ethylenedioxythiophene)

Sensors ◽  
2018 ◽  
Vol 18 (11) ◽  
pp. 3684 ◽  
Author(s):  
Yesong Gu ◽  
Po-Yuan Tseng ◽  
Xiang Bi ◽  
Jason Yang
Keyword(s):  
Gold Nanoparticles ◽  
Limit Of Detection ◽  
Differential Pulse ◽  
Complementary Sequence ◽  
Signal Decay ◽  
Target Dna ◽  
Hybridization Efficiency ◽  
Sh Group ◽  
Polymerase Chain ◽  
Or Gene

The general clinical procedure for viral DNA detection or gene mutation diagnosis following polymerase chain reaction (PCR) often involves gel electrophoresis and DNA sequencing, which is usually time-consuming. In this study, we have proposed a facile strategy to construct a DNA biosensor, in which the platinum electrode was modified with a dual-film of electrochemically synthesized poly(3,4-ethylenedioxythiophene) (PEDOT) resulting in immobilized gold nanoparticles, with the gold nanoparticles easily immobilized in a uniform distribution. The DNA probe labeled with a SH group was then assembled to the fabricated electrode and employed to capture the target DNA based on the complementary sequence. The hybridization efficiency was evaluated with differential pulse voltammetry (DPV) in the presence of daunorubicin hydrochloride. Our results demonstrated that the peak current in DPV exhibited a linear correlation the concentration of target DNA that was complementary to the probe DNA. Moreover, the electrode could be reused by heating denaturation and re-hybridization, which only brought slight signal decay. In addition, the addition of the oxidized form of nicotinamide adenine dinucleotide (NAD+) could dramatically enhance the sensitivity by more than 5.45-fold, and the limit-of-detection reached about 100 pM.

Simulation of Gold Nanoparticles Aggravating MEMS Cantilever Optical Static Detection Biochip

2013 ◽  
Vol 694-697 ◽  
pp. 966-970 ◽  
Author(s):  
Yue Tao Ge ◽  
Xiao Tong Yin
Keyword(s):  
Gold Nanoparticles ◽  
Low Cost ◽  
Mechanical Systems ◽  
Side Wall ◽  
High Sensitivity ◽  
Detection Sensitivity ◽  
Gene Detection ◽  
Micro Electro Mechanical Systems ◽  
Target Dna ◽  
Static Detection

A kind of gene detection biochip model based on biological micro electro mechanical systems (BioMEMS) technology and micro optical electro mechanical systems (MOEMS) technology is designed and simulated. In order to detect whether there are nucleic acid components in the testing samples, the biochip in this study issues horizontal light by laser, then receives and reads the deformation signals of MEMS cantilever by optical detector. The MEMS optical reflecting system can amplify MEMS cantilever deformation signal 22 times by micro reflectors which are set on the side wall of the cantilever free end. In order to improve optical detection sensitivity, gold nanoparticles (GNPs) which are combined with hybridization information is taken to aggravate MEMS cantilever, and employ Au - S chemical bond of GNPs and dithiol HS(CH2)6SH to combine and fix DNA probe, and then employ target DNA which is marked with biotin to combine GNPs by Biotin - Streptavidin combining. The simulation results show that this biochip can detect biological samples fast, high throughput, low cost, high sensitivity and reliably.

A Novel Inductive Coupled Plasma Mass Spectrometry Gene Detection Method Based on AuNPs and Bio-Barcode Signal Amplification

2019 ◽  
Vol 11 (5) ◽  
pp. 638-644 ◽  
Author(s):  
Pengfei Jiang ◽  
Chomba Haji ◽  
Xiufang Ye ◽  
Menglei Chang ◽  
Wenjun Li ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Signal Amplification ◽  
Limit Of Detection ◽  
High Sensitivity ◽  
Mismatched Dna ◽  
Icp Ms ◽  
Target Dna ◽  
Cancer Related Gene ◽  
Plasma Mass Spectrometry ◽  
Double Base

An inductive coupled plasma mass spectrometry (ICP-MS) method based on gold nanoparticles (AuNPs) and bio-barcode signal amplification was presented for the sensitive detection of gastric cancer related gene. The target DNA, magnetic nanoprobes and SiO2/AuNPs barcode probes were hybridized to form a "sandwich" structure, then a large amount of AuNPs which were amplified by bio-barcode technology were detected by ICP-MS. This method presented a limit of detection as low as 1 fM. The ratio of the background-subtracted 197 Au signals for totally complementary DNA, single-base mismatched DNA, double-base mismatched DNA and totally mismatched DNA was 115:30:16:1, respectively. It's suggested that the complementary and mismatched DNA can be distinguished clearly. This novel ICP-MS biosensor is prospective in DNA detection with high sensitivity and specificity.

The Role of Adhesion in Gold Nanoparticles for Cancer Detection and Treatment

2015 ◽  
Vol 1132 ◽  
pp. 72-86
Author(s):  
Y. Oni ◽  
J.D. Obayemi ◽  
K. Kao ◽  
S. Dozie-Nwachukwu ◽  
S. Odusanya ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Gold Nanoparticles ◽  
Cancer Cells ◽  
Cancer Detection ◽  
Breast Cancer Cells ◽  
Au Nanoparticle ◽  
Adhesion Forces ◽  
Force Microscopy ◽  
Nanoparticle Clusters ◽  
Potential Applications

This paper presents the results of an experimental study of the effects of adhesion between gold nanoparticles and surfaces that are relevant to the potential applications in cancer detection and treatment. Adhesion is measured using a dip coating/atomic force microscopy (DC/AFM) technique. The adhesion forces are obtained for dip-coated gold nanoparticles that interact with peptide or antibody-based molecular recognition units (MRUs) that attach specifically to breast cancer cells. They include MRUs that attach specifically to receptors on breast cancer cells. Adhesion forces between anti-cancer drugs such as paclitaxel, and the constituents of MRU-conjugated Au nanoparticle clusters, are measured using force microscopy techniques. The implications of the results are then discussed for the design of robust gold nanoparticle clusters and for potential applications in localized drug delivery and hyperthermia.

Nanogold Aggravating MEMS Cantilever Capacitance Detection Biochip

2010 ◽  
Vol 159 ◽  
pp. 429-433 ◽  
Author(s):  
Zhong Liang Deng ◽  
Yue Tao Ge ◽  
Wei Guo Guan ◽  
Nai Bo Zhang ◽  
Qi Ke Cao
Keyword(s):  
Gold Nanoparticles ◽  
Integrated Circuit ◽  
Low Cost ◽  
High Sensitivity ◽  
Detection Sensitivity ◽  
Capacitive Pressure Sensor ◽  
Target Dna ◽  
Capacitance Detection ◽  
Before And After ◽  
Hybridization Reaction

A kind of array micro-electromechanical systems (MEMS) cantilever of biochip is designed, which integrated capacitive pressure sensor. Before and after hybridization reaction, by the change of capacitance value, it can measure the capacitance values through integrated circuit (IC) to judge whether the solution containing the cantilever probe genes. In order to improve the detection sensitivity, it aggravate cantilever by gold nanoparticles combining hybridization information, applying Au-S chemical bond of gold nanoparticles and dithiol HS(CH2)6SH to combine and fix DNA probes and applying target DNA marked with biotin to combine gold nanoparticles by Biotin - Streptavidin combining. The results shows that this biochip can detect biological samples fast, high throughput, low cost, high sensitivity and reliably.

Amplified Electrochemical DNA Sensor Based on Polyaniline Film and Gold Nanoparticles

2013 ◽  
Vol 25 (6) ◽  
pp. 1373-1380 ◽  
Author(s):  
Reyhaneh-Sadat Saberi ◽  
Saeed Shahrokhian ◽  
Giovanna Marrazza
Keyword(s):  
Gold Nanoparticles ◽  
Dna Sensor ◽  
Polyaniline Film ◽  
Electrochemical Dna Sensor
Sign in / Sign up

Export Citation Format

Share Document