Production of anti-inflammatory compounds in Sphaeralcea angustifolia
 cell suspension cultivated in stirred tank bioreactor

Bursera linanoe cell suspension cultures were initiated from callus grown in Murashige and Skoog medium supplemented with naphthalene acetic acid (3.0 mg L−1) and 6-benzylaminopurine (0.5 mg L−1). In flasks, B. linanoe cell cultures grew over a 9 day period, reaching a maximum biomass of 11.16 g DW L−1. Throughout the growth phase, cell viability was constant at 60 – 70%. In contrast, B. linanoe cells growing in a bioreactor achieved a maximum biomass of 22.26 g DW L−1 (after 7 days), and cell viability was constant at 75 - 85%. Production of linalool and linalyl acetate in the bioreactor (3.02 and 2.40 mg g−1 DW, respectively) was significantly greater than that achieved from cells in flask cultures (1.05 and 0.97 mg g−1 DW, respectively). B. linanoe cell suspension culture has potential as an alternative method for the production of essential oils.

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Dynamical simulation of a continuous stirred tank bioreactor with the use of cellular automata for the biofilm description

Chemical Engineering Research and Design ◽

10.1016/j.cherd.2021.02.019 ◽

2021 ◽

Vol 168 ◽

pp. 264-274

Author(s):

Szymon Skoneczny ◽

Monika Cioch-Skoneczny

Keyword(s):

Cellular Automata ◽

Stirred Tank ◽

Stirred Tank Bioreactor ◽

Dynamical Simulation ◽

Continuous Stirred Tank

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High level production of laccases and peroxidases from the newly isolated white-rot basidiomycete Marasmiellus palmivorus VE111 in a stirred-tank bioreactor in response to different carbon and nitrogen sources

Process Biochemistry ◽

10.1016/j.procbio.2018.03.005 ◽

2018 ◽

Vol 69 ◽

pp. 1-11 ◽

Cited By ~ 15

Author(s):

Willian Daniel Hahn Schneider ◽

Roselei Claudete Fontana ◽

Simone Mendonça ◽

Félix Gonçalves de Siqueira ◽

Aldo José Pinheiro Dillon ◽

...

Keyword(s):

Nitrogen Sources ◽

White Rot ◽

Stirred Tank ◽

Carbon And Nitrogen Sources ◽

Stirred Tank Bioreactor ◽

Carbon And Nitrogen ◽

High Level ◽

Level Production

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Utilisation of winery wastewater for xanthan production in stirred tank bioreactor: Bioprocess modelling and optimisation

Food and Bioproducts Processing ◽

10.1016/j.fbp.2019.06.019 ◽

2019 ◽

Vol 117 ◽

pp. 113-125 ◽

Cited By ~ 3

Author(s):

Zorana Rončević ◽

Jovana Grahovac ◽

Siniša Dodić ◽

Damjan Vučurović ◽

Jelena Dodić

Keyword(s):

Stirred Tank ◽

Stirred Tank Bioreactor ◽

Winery Wastewater ◽

Xanthan Production

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Production of Newcastle Disease Virus by Vero Cells Grown on Cytodex 1 Microcarriers in a 2-Litre Stirred Tank Bioreactor

Journal of Biomedicine and Biotechnology ◽

10.1155/2010/586363 ◽

2010 ◽

Vol 2010 ◽

pp. 1-7 ◽

Cited By ~ 15

Author(s):

Mohd Azmir Arifin ◽

Maizirwan Mel ◽

Mohamed Ismail Abdul Karim ◽

Aini Ideris

Keyword(s):

Cell Culture ◽

Newcastle Disease Virus ◽

Disease Virus ◽

Newcastle Disease ◽

High Speed ◽

Virus Titer ◽

Vero Cells ◽

Stirred Tank ◽

Stirred Tank Bioreactor ◽

Maximum Cell

The aim of this study is to prepare a model for the production of Newcastle disease virus (NDV) lentogenic F strain using cell culture in bioreactor for live attenuated vaccine preparation. In this study, firstly we investigated the growth of Vero cells in several culture media. The maximum cell number was yielded by culture of Vero cells in Dulbecco's Modified Eagle Medium (DMEM) which was1.93×106 cells/ml. Secondly Vero cells were grown in two-litre stirred tank bioreactor by using several commercial microcarriers. We achieved the maximum cell concentration about7.95×105 cells/ml when using Cytodex 1. Later we produced Newcastle Disease virus in stirred tank bioreactor based on the design developed using Taguchi L4 method. Results reveal that higher multiplicity of infection (MOI) and size of cell inoculums can yield higher virus titer. Finally, virus samples were purified using high-speed centrifugation based on3∗∗(3-1) Fractional Factorial Design. Statistical analysis showed that the maximum virus titer can be achieved at virus sample concentration of 58.45% (v/v), centrifugation speed of 13729 rpm, and centrifugation time of 4 hours. As a conclusion, high yield of virus titer could be achieved through optimization of cell culture in bioreactor and separation by high-speed centrifugation.

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