scholarly journals Human colon cancer epithelial cells harbour active HEDGEHOG‐GLI signalling that is essential for tumour growth, recurrence, metastasis and stem cell survival and expansion

2009 ◽  
Vol 1 (6‐7) ◽  
pp. 338-351 ◽  
Author(s):  
Frédéric Varnat ◽  
Arnaud Duquet ◽  
Monica Malerba ◽  
Marie Zbinden ◽  
Christophe Mas ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Stem Cell ◽  
Epithelial Cells ◽  
Cell Survival ◽  
Tumour Growth ◽  
Human Colon ◽  
Human Colon Cancer ◽  
Stem Cell Survival

scholarly journals The Importance of the Stem Cell Marker Prominin-1/CD133 in the Uptake of Transferrin and in Iron Metabolism in Human Colon Cancer Caco-2 Cells

PLoS ONE ◽  
2011 ◽  
Vol 6 (9) ◽  
pp. e25515 ◽  
Author(s):  
Erika Bourseau-Guilmain ◽  
Audrey Griveau ◽  
Jean-Pierre Benoit ◽  
Emmanuel Garcion
Keyword(s):  
Colon Cancer ◽  
Stem Cell ◽  
Iron Metabolism ◽  
Human Colon ◽  
Stem Cell Marker ◽  
Cell Marker ◽  
Human Colon Cancer

scholarly journals Generation of an inducible colon-specific Cre enzyme mouse line for colon cancer research

2016 ◽  
Vol 113 (42) ◽  
pp. 11859-11864 ◽  
Author(s):  
Paul W. Tetteh ◽  
Kai Kretzschmar ◽  
Harry Begthel ◽  
Maaike van den Born ◽  
Jeroen Korving ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Small Intestine ◽  
Epithelial Cells ◽  
Mouse Model ◽  
Human Colon ◽  
Suppressor Gene ◽  
Proximal Colon ◽  
Tumor Formation ◽  
Cell Of Origin ◽  
Human Colon Cancer

Current mouse models for colorectal cancer often differ significantly from human colon cancer, being largely restricted to the small intestine. Here, we aim to develop a colon-specific inducible mouse model that can faithfully recapitulate human colon cancer initiation and progression. Carbonic anhydrase I (Car1) is a gene expressed uniquely in colonic epithelial cells. We generated a colon-specific inducible Car1CreER knock-in (KI) mouse with broad Cre activity in epithelial cells of the proximal colon and cecum. Deletion of the tumor suppressor gene Apc using the Car1CreER KI caused tumor formation in the cecum but did not yield adenomas in the proximal colon. Mutation of both Apc and Kras yielded microadenomas in both the cecum and the proximal colon, which progressed to macroadenomas with significant morbidity. Aggressive carcinomas with some invasion into lymph nodes developed upon combined induction of oncogenic mutations of Apc, Kras, p53, and Smad4. Importantly, no adenomas were observed in the small intestine. Additionally, we observed tumors from differentiated Car1-expressing cells with Apc/Kras mutations, suggesting that a top-down model of intestinal tumorigenesis can occur with multiple mutations. Our results establish the Car1CreER KI as a valuable mouse model to study colon-specific tumorigenesis and metastasis as well as cancer-cell-of-origin questions.

scholarly journals Oncogenic KRAS activates an embryonic stem cell-like program in human colon cancer initiation

Oncotarget ◽  
2016 ◽  
Vol 7 (3) ◽  
pp. 2159-2174 ◽  
Author(s):  
Anne-France Le Rolle ◽  
Thang K. Chiu ◽  
Zhaoshi Zeng ◽  
Jinru Shia ◽  
Martin R. Weiser ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Embryonic Stem ◽  
Human Colon ◽  
Human Colon Cancer ◽  
Cancer Initiation
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