Arsenic speciation and effect of arsenate inhibition in a Microcystis aeruginosa culture medium under different phosphate regimes

The laboratory experiment was conducted to investigated the effect of nickel over a concentration gradient of 0.1~1.00 mg/L on biomass ( indicated by absorbance of cell culture at 663nm wavelength ), superoxide anion (O2•ˉ), malondialdehyde (MDA), and superoxide dismutase (SOD) in the cyanobacterium Microcystis aeruginosa FACHB 905 isolated from Dianchi Lake, south west of China . The results showed: (1) M. aeruginosa could live normally in Ni (II)-absence culture medium，but 0.3 mg/L Ni(II) was the fittest concentration for test M. aeruginosa, in which biomass and SOD activity were highest among all test cultures; (2) Ni(II) induced O2•ˉ and MDA in M. aeruginosa cells under the experimental condition, showing that Ni(II) could influence on M. aeruginosa by inducing oxidative stress; (3) Fe-SOD and Mn-SOD were found in M. aeruginosa and both of them were induced by lower Ni(II) but inhibited by higher concentration. The multiformity of SOD isoenzymes enhance the resistance of M. aeruginosa to oxygen stress induced by unfavorable condition, which explained that M. aeruginosa is the preponderant species in badly polluted Dianchi Lake water for long period.

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Toxicity of The Microcystin-Producing Cyanobacterium Microcystis Aeruginosa To Litopenaeus Vannamei

10.21203/rs.3.rs-1220648/v1 ◽

2022 ◽

Author(s):

Yu Xu ◽

Wujie Xu ◽

Xiaojuan Hu ◽

Haochang Su ◽

Guoliang Wen ◽

...

Keyword(s):

Immune Response ◽

High Pressure ◽

Antibacterial Activity ◽

Microcystis Aeruginosa ◽

Litopenaeus Vannamei ◽

Hplc Analysis ◽

Culture Medium ◽

Reduced Glutathione ◽

Cell Extract ◽

Negative Effect

Abstract Microcystis aeruginosa is a common kind of harmful bloom algae, which was also frequently found as a dominant microalgae specie in shrimp breeding ponds. And it was found that blooms always induced massive death of shrimp, but the toxic effects of M. aeruginosa on Litopenaeus vannamei are still not completely understood. In this paper, the toxicity of M. aeruginosa cells to L. vannamei was examined, and the toxic components in the cells were analyzed through high-pressure liquid chromatography (HLPC). In addition, the immune response of shrimp to the microalgal extract was assessed by measuring the activity of immune-related enzymes, as well as the transcription of the relevant genes. Overall, both M. aeruginosa cells and the algal extract resulted in a 100% mortality rate in shrimp, whereas the cell-free culture medium was ineffective. And HPLC analysis results revealed the presence of microcystin-LR (MC-LR) at a concentration of 190.40 mg/kg of cells. In addition, the activity and gene transcription of two immune related enzymes, SOD and LZM, were both significantly reduced in shrimp hepatopancreas (p<0.05) after injection with cell extract. However, reduced glutathione (GSH) content was slightly increased, but the ratio of GSH to GSSG was down, and the transcription of gst gene function as detoxification, was significantly downregulated (p<0.05). The results demonstrated that M. aeruginosa cell extract was highly toxic to L. vannamei, and exerted a negative effect on shrimp immunity including reduction of antioxidant capacity, antibacterial activity and detoxification activity, due to microcystin-LR.

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Simultaneous Cultivation of Spirulina platensis and the Toxigenic Cyanobacteria Microcystis aeruginosa

Zeitschrift für Naturforschung C ◽

10.1515/znc-2006-1-219 ◽

2006 ◽

Vol 61 (1-2) ◽

pp. 105-110 ◽

Cited By ~ 19

Author(s):

Jorge Alberto Vieira Costa ◽

Michele Greque de Morais ◽

Francieli Dalcanton ◽

Carolina da Cruz Reichert ◽

Andrei José Durante

Keyword(s):

Microcystis Aeruginosa ◽

Spirulina Platensis ◽

Culture Medium ◽

Biomass Concentration ◽

Physicochemical Characteristics ◽

Production Costs ◽

Lagoon Water ◽

23 Factorial Design ◽

Initial Biomass Concentration ◽

South Of Brazil

Abstract Mangueira Lagoon, located in the extreme south of Brazil, has water with physicochemical characteristics such as alkaline pH and carbonate levels propitious for the growth of the cyanobacterium Spirulina platensis. Previously published studies have shown that Mangueira Lagoon water supplemented with small quantities of carbon and nitrogen is suitable for S. platensis cultivation and can significantly reduce production costs. We studied mixed cultures of Spirulina platensis and the toxic cyanobacterium Microcystis aeruginosa using a 23 factorial design in which the three factors were the initial biomass concentration of S. platensis and M. aeruginosa and the type of culture medium (100% Zarrouk’s medium or 80% Mangueira Lagoon water plus 20% Zarrouk’s medium). The highest S. platensis maximum specific growth rate (μmax) occurred in the culture with the highest M. aeruginosa biomass concentration and when undiluted culture medium was used (μmax = 0.283 d-1). The highest M. aeruginosa specific death rate (k) was obtained in the presence of S. platensis (k = 0.555 d-1) and was independent of the initial M. aeruginosa biomass concentration and culture medium, demonstrating that S. platensis cultures are not susceptible to contamination by M. aeruginosa. The culture medium had no significant influence (p > 0.05) on S. platensis μmax values, indicating that production costs could be reduced by using a medium consisting of 80% Mangueira Lagoon water plus 20% Zarrouk’s medium

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Arsenic speciation and biotransformation by the marine macroalga Undaria pinnatifida in seawater: A culture medium study

Chemosphere ◽

10.1016/j.chemosphere.2019.01.185 ◽

2019 ◽

Vol 222 ◽

pp. 705-713 ◽

Cited By ~ 2

Author(s):

M. Abdullah Al Mamun ◽

Ismail M.M. Rahman ◽

Rakhi Rani Datta ◽

Chika Kosugi ◽

Asami S. Mashio ◽

...

Keyword(s):

Culture Medium ◽

Undaria Pinnatifida ◽

Arsenic Speciation ◽

Medium Study ◽

Marine Macroalga

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Effect of Fe2+ and Fe 3+ on the Growth of Microcystis aeruginosa, Scenedesmus quadricauda and Cyclotella sp.

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.183-185.510 ◽

2011 ◽

Vol 183-185 ◽

pp. 510-515

Author(s):

Qin Wang ◽

Zong Xue Xu ◽

Xia Jiang ◽

Ji Xi Gao

Keyword(s):

Microcystis Aeruginosa ◽

Culture Medium ◽

Growth Curves ◽

Scenedesmus Quadricauda ◽

Logistic Growth ◽

High Concentration ◽

Nutrient Sources ◽

Monod Equation ◽

Maximum Biomass ◽

Logistic Growth Model

Ferrum is one of the important nutrient sources for algae in lakes. The changes of concentration in water body have great effect on the formation of dominant algae. Microcystis aeruginosa, Scenedesmus quadricauda and Cyclotella were examined in M11 culture medium which included different Fe2+ and Fe3+ concentration. Growth curves of these three algae were fitted by logistic growth-model, respectively. The maximum biomass K, inflection t and growth rate µ of those algae were investigated. The effects of Fe2+ and Fe3+ on the growth of algae were investigated by using the Monod equation. The semi-saturation constants were calculated. The results showed that the maximum biomass of these three algae did not increase with the increasing of Fe2+ and Fe3+ concentration. Low concentration of Fe2+ (200-500 mg/L) and high concentration of Fe3+ (2000-5000 mg/L) were more suitable to the growth of three algae. The sequence of the maximum biomass was: Scenedesmus quadricauda>Microcystis aeruginosa>Cyclotella.

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Effect of culture age and pH of the culture medium on the composition of the toxin of the cyanobacterium Microcystis aeruginosa (UV-006)

South African Journal of Botany ◽

10.1016/s0254-6299(16)31304-7 ◽

1988 ◽

Vol 54 (4) ◽

pp. 372-374 ◽

Cited By ~ 2

Author(s):

A.J. van der Westhuizen ◽

G.H.J. Krüger ◽

J.N. Eloff

Keyword(s):

Microcystis Aeruginosa ◽

Culture Medium ◽

Culture Age

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Fate and effects of octylphenol in a Microcystis aeruginosa culture medium

Aquatic Toxicology ◽

10.1016/j.aquatox.2008.12.005 ◽

2009 ◽

Vol 92 (2) ◽

pp. 59-64 ◽

Cited By ~ 25

Author(s):

Mafalda S. Baptista ◽

Teodor Stoichev ◽

M. Clara P. Basto ◽

Vitor M. Vasconcelos ◽

M.Teresa S.D. Vasconcelos

Keyword(s):

Microcystis Aeruginosa ◽

Culture Medium

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Allelopathic effects of the aquatic macrophyte Ceratophyllum demersum L. on phytoplankton species: contrasting effects between cyanobacteria and chlorophytes

Acta Limnologica Brasiliensia ◽

10.1590/s2179-975x1419 ◽

2019 ◽

Vol 31 ◽

Cited By ~ 6

Author(s):

Cihelio Alves Amorim ◽

Rafael Henrique de Moura-Falcão ◽

Celina Rebeca Valença ◽

Vitor Ricardo de Souza ◽

Ariadne do Nascimento Moura

Keyword(s):

Microcystis Aeruginosa ◽

Growth Rates ◽

Culture Medium ◽

Aquatic Macrophyte ◽

Cyanobacterial Blooms ◽

Ceratophyllum Demersum ◽

Phytoplankton Species ◽

Raphidocelis Subcapitata ◽

Ankistrodesmus Falcatus ◽

Allelopathic Effects

Abstract Aim To assess the allelopathic effects of the submerged macrophyte Ceratophyllum demersum on four strains of phytoplankton species: two cyanobacteria (Microcystis aeruginosa - microcystin producing and M. panniformis - non-microcystin producing), and two chlorophytes (Ankistrodesmus falcatus and Raphidocelis subcapitata). Methods A coexistence experiment between C. demersum and the four strains was carried out for six days, with eight treatments and three replicates. The strains were cultivated in ASM1 culture medium, under controlled laboratory conditions. Two treatments were assigned for each strain, one with 6 g.L-1 of the macrophyte, and the control without the plant. Biomasses and growth rates of the strains were evaluated every two days, which were compared through the T-test and two-way ANOVA, respectively. Results The results varied among the strains, with toxic M. aeruginosa being intensely inhibited by C. demersum, with a decrease of 99.5% in its biomass (p<0.001), while non-toxic M. panniformis was less affected by the allelochemicals, with a reduction of 86.2% (p<0.001). Ankistrodesmus falcatus delayed its growth when in coexistence with the macrophyte, decreasing its biomass in 50.4% (p<0.01), while R. subcapitata was not altered (p>0.05). In coexistence with C. demersum, M. aeruginosa exhibited the lowest growth rates (-0.65 d-1), followed by M. panniformis (-0.15 d-1), A. falcatus (0.19 d-1), and R. subcapitata (0.34 d-1), with significant differences between all strains (p<0.001). Microcystis aeruginosa presented higher inhibition rates than M. panniformis (p<0.001), as well as, A. falcatus was more inhibited than R. subcapitata (p<0.05). Conclusions The presence of microcystins could influence the allelopathic responses of C. demersum, that may release more allelochemicals in coexistence with toxic strains of M. aeruginosa. Accordingly, C. demersum can be used in biomanipulation strategies to control toxic and non-toxic cyanobacterial blooms, without damaging other phytoplankton species, like chlorophytes.

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Effects of Cellular Metabolism and Viability on Metal Ion Accumulation by Cultured Biomass from a Bloom of the Cyanobacterium Microcystis aeruginosa

Applied and Environmental Microbiology ◽

10.1128/aem.64.4.1545-1547.1998 ◽

1998 ◽

Vol 64 (4) ◽

pp. 1545-1547 ◽

Cited By ~ 27

Author(s):

D. L. Parker ◽

L. C. Rai ◽

N. Mallick ◽

P. K. Rai ◽

H. D. Kumar

Keyword(s):

Microcystis Aeruginosa ◽

Metal Ion ◽

Culture Medium ◽

Cellular Metabolism ◽

Ion Accumulation ◽

Metabolic Inhibitors ◽

Copper Accumulation ◽

Cells In Culture ◽

Naturally Occurring ◽

Nickel Cadmium

ABSTRACT The sorption of nickel, cadmium, and copper by cultured biomass from a naturally occurring bloom of Microcystis aeruginosawas demonstrated in two systems: cells suspended in culture medium and cells immobilized in alginate. Incubation in the absence of light, in the presence of metabolic inhibitors, and at 4°C did not substantially decrease the copper accumulation by cells in culture medium. Heat-killed, formaldehyde-treated, and air-dried biomass samples sorbed nearly as much (or in some cases slightly more) copper as did viable samples.

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