Kinetics of indocyanine green: Optimizing tumor to normal tissue fluorescence in image‐guided oral cancer surgery applications

Head & Neck ◽  
2018 ◽  
Vol 41 (4) ◽  
pp. 1032-1038 ◽  
Author(s):  
Yuxin Wang ◽  
Diya Xie ◽  
Ziyang Wang ◽  
Xudong Zhang ◽  
Qian Zhang ◽  
...  
Keyword(s):  
Oral Cancer ◽  
Indocyanine Green ◽  
Normal Tissue ◽  
Cancer Surgery ◽  
Image Guided ◽  
Tissue Fluorescence ◽  
Kinetics Of

scholarly journals Paired-Agent Fluorescence Molecular Imaging of Sentinel Lymph Nodes Using Indocyanine Green as a Control Agent for Antibody-Based Targeted Agents

2019 ◽  
Vol 2019 ◽  
pp. 1-13 ◽  
Author(s):  
Chengyue Li ◽  
Xiaochun Xu ◽  
Nathan McMahon ◽  
Omar Alhaj Ibrahim ◽  
Husain A. Sattar ◽  
...  
Keyword(s):  
Molecular Imaging ◽  
Lymph Nodes ◽  
Indocyanine Green ◽  
Metastatic Breast ◽  
Control Agent ◽  
Sentinel Lymph Nodes ◽  
Imaging Agent ◽  
Binding Potential ◽  
Cancer Spread ◽  
Kinetics Of

Purpose. Paired-agent molecular imaging methods, which employ coadministration of an untargeted, “control” imaging agent with a targeted agent to correct for nonspecific uptake, have been demonstrated to detect 200 cancer cells in a mouse model of metastatic breast cancer. This study demonstrates that indocyanine green (ICG), which is approved for human use, is an ideal control agent for future paired-agent studies to facilitate eventual clinical translation. Methods. The kinetics of ICG were compared with a known ideal control imaging agent, IRDye-700DX-labeled antibody in both healthy and metastatic rat popliteal lymph nodes after coadministration, intradermally in the footpad. Results. The kinetics of ICG and antibody-based imaging agent in tumor-free rat lymph nodes demonstrated a strong correlation with each other (r = 0.98, p<0.001) with a measured binding potential of −0.102 ± 0.03 at 20 min postagent injection, while the kinetics of ICG and targeted imaging agent shows significant separation in the metastatic lymph nodes. Conclusion. This study indicated a potential for microscopic sensitivity to cancer spread in sentinel lymph nodes using ICG as a control agent for antibody-based molecular imaging assays.

Microbiology of wound infection after oral cancer surgery

2020 ◽  
Vol 48 (7) ◽  
pp. 700-705
Author(s):  
Margita Belusic-Gobic ◽  
Arijan Zubovic ◽  
Anamarija Predrijevac ◽  
David Harmicar ◽  
Robert Cerovic ◽  
...  
Keyword(s):  
Oral Cancer ◽  
Wound Infection ◽  
Cancer Surgery

scholarly journals Indocyanine Green-Loaded Nanoparticles for Image-Guided Tumor Surgery

2015 ◽  
Vol 26 (2) ◽  
pp. 294-303 ◽  
Author(s):  
Tanner K. Hill ◽  
Asem Abdulahad ◽  
Sneha S. Kelkar ◽  
Frank C. Marini ◽  
Timothy E. Long ◽  
...  
Keyword(s):  
Indocyanine Green ◽  
Tumor Surgery ◽  
Image Guided

Use of dichromatic ear densitometry to evaluate kinetics of indocyanine green (ICG) removal in children with liver disease

1965 ◽  
Vol 67 (5) ◽  
pp. 964
Author(s):  
Mervin Silverberg ◽  
Peter Z. Neumann ◽  
Maceo M. Howard ◽  
Carroll M. Leevy
Keyword(s):  
Liver Disease ◽  
Indocyanine Green ◽  
Kinetics Of

scholarly journals Digital dynamic discrimination of primary colorectal cancer using systemic indocyanine green with near-infrared endoscopy

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Jeffrey Dalli ◽  
Eamon Loughman ◽  
Niall Hardy ◽  
Anwesha Sarkar ◽  
Mohammad Faraz Khan ◽  
...  
Keyword(s):  
Indocyanine Green ◽  
Real Time ◽  
Normal Tissue ◽  
Near Infrared ◽  
Malignant Lesion ◽  
Fluorescence Analysis ◽  
Video Tracking ◽  
Continuous Stream ◽  
Rich Data

AbstractAs indocyanine green (ICG) with near-infrared (NIR) endoscopy enhances real-time intraoperative tissue microperfusion appreciation, it may also dynamically reveal neoplasia distinctively from normal tissue especially with video software fluorescence analysis. Colorectal tumours of patients were imaged mucosally following ICG administration (0.25 mg/kg i.v.) using an endo-laparoscopic NIR system (PINPOINT Endoscopic Fluorescence System, Stryker) including immediate, continuous in situ visualization of rectal lesions transanally for up to 20 min. Spot and dynamic temporal fluorescence intensities (FI) were quantified using ImageJ (including videos at one frame/second, fps) and by a bespoke MATLAB® application that provided digitalized video tracking and signal logging at 30fps (Fluorescence Tracker App downloadable via MATLAB® file exchange). Statistical analysis of FI-time plots compared tumours (benign and malignant) against control during FI curve rise, peak and decline from apex. Early kinetic FI signal measurement delineated discriminative temporal signatures from tumours (n = 20, 9 cancers) offering rich data for analysis versus delayed spot measurement (n = 10 cancers). Malignant lesion dynamic curves peaked significantly later with a shallower gradient than normal tissue while benign lesions showed significantly greater and faster intensity drop from apex versus cancer. Automated tracker quantification efficiently expanded manual results and provided algorithmic KNN clustering. Photobleaching appeared clinically irrelevant. Analysis of a continuous stream of intraoperatively acquired early ICG fluorescence data can act as an in situ tumour-identifier with greater detail than later snapshot observation alone. Software quantification of such kinetic signatures may distinguish invasive from non-invasive neoplasia with potential for real-time in silico diagnosis.

PMMC WITH TWO SEPARATE SKIN ISLANDS -AN UNIQUE TECHNIQUE AND ITS USE IN COMPLEX HEAD AND NECK DEFECTS

2021 ◽  
pp. 76-78
Author(s):  
Siddappa K.Thammaiah ◽  
Samskruthi P Murthy ◽  
P Krishna Prasad ◽  
Rajshekar Halkud ◽  
Purushottham Chavan ◽  
...  
Keyword(s):  
Oral Cancer ◽  
Cancer Patients ◽  
Buccal Mucosa ◽  
Normal Tissue ◽  
Advanced Disease ◽  
Radical Neck Dissection ◽  
Advanced Stage ◽  
Skin Involvement ◽  
Oral Cancer Patients ◽  
Unique Manner

More than two thirds of oral cancer patients present in advanced stage in India, from subsites like Buccal mucosa and tongue often present with N3b nodes with skin involvement. This type of advanced disease usually requires composite resection and extended radical neck dissection. If both the defects are adjacent to each other can be addressed by bipaddle PMMC. If the signicant normal tissue lies between the primary and neck defects [level 2b,3,5] it should be Reconstruction with a single free ap or combination of free and locoregional aps. Free ap reconstruction requires expertise and other ancillary instruments which is difcult to organize in COVID situation. We overcame this problem by designing the PMMC in a unique manner with two island skin paddles based on the pectoral branch of thoraco-acromial vessels to cover both the defects.

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