Quantitative detection of hepatitis C virus RNA with a solid-phase signal amplification method: Definition of optimal conditions for specimen collection and clinical application in interferon-treated patients

Hepatology ◽  
1994 ◽  
Vol 19 (6) ◽  
pp. 1337-1341 ◽  
Author(s):  
Gary L. Davis ◽  
Johnson Y.-N. Lau ◽  
Mickie S. Urdea ◽  
Paul D. Neuwald ◽  
Judith C. Wilber ◽  
...  
Keyword(s):  
Signal Amplification ◽  
Solid Phase ◽  
Quantitative Detection ◽  
Optimal Conditions ◽  
Hepatitis C Virus Rna ◽  
Amplification Method ◽  
Specimen Collection ◽  
Virus Rna ◽  
Phase Signal ◽  
Definition Of

scholarly journals SARS-CoV-2 RNA Detection with Duplex-Specific Nuclease Signal Amplification

Micromachines ◽  
2021 ◽  
Vol 12 (2) ◽  
pp. 197
Author(s):  
Meiqing Liu ◽  
Haoran Li ◽  
Yanwei Jia ◽  
Pui-In Mak ◽  
Rui P. Martins
Keyword(s):  
Signal Amplification ◽  
Incubation Temperature ◽  
Dna Probes ◽  
Detection Sensitivity ◽  
N Gene ◽  
Rna Detection ◽  
Gold Standard Method ◽  
Complementary Method ◽  
Amplification Method ◽  
Virus Rna

The emergence of the novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), a zoonotic pathogen, has led to the outbreak of coronavirus disease 2019 (COVID-19) pandemic and brought serious threats to public health worldwide. The gold standard method for SARS-CoV-2 detection requires both reverse transcription (RT) of the virus RNA to cDNA and then polymerase chain reaction (PCR) for the cDNA amplification, which involves multiple enzymes, multiple reactions and a complicated assay optimization process. Here, we developed a duplex-specific nuclease (DSN)-based signal amplification method for SARS-CoV-2 detection directly from the virus RNA utilizing two specific DNA probes. These specific DNA probes can hybridize to the target RNA at different locations in the nucleocapsid protein gene (N gene) of SARS-CoV-2 to form a DNA/RNA heteroduplex. DSN cleaves the DNA probe to release fluorescence, while leaving the RNA strand intact to be bound to another available probe molecule for further cleavage and fluorescent signal amplification. The optimized DSN amount, incubation temperature and incubation time were investigated in this work. Proof-of-principle SARS-CoV-2 detection was demonstrated with a detection sensitivity of 500 pM virus RNA. This simple, rapid, and direct RNA detection method is expected to provide a complementary method for the detection of viruses mutated at the PCR primer-binding regions for a more precise detection.

Quantification of hepatitis C virus RNA in serum by branched DNA-based signal amplification assays

1999 ◽  
Vol 79 (2) ◽  
pp. 227-235 ◽  
Author(s):  
Jean-Michel Pawlotsky ◽  
Michèle Martinot-Peignoux ◽  
Jean-Dominique Poveda ◽  
Anne Bastie ◽  
Véronique Le Breton ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Signal Amplification ◽  
Hepatitis C Virus Rna ◽  
Branched Dna ◽  
Virus Rna

Impact of various handling and storage conditions on quantitative detection of hepatitis C virus RNA

1996 ◽  
Vol 25 (3) ◽  
pp. 307-311 ◽  
Author(s):  
Philippe Halfon ◽  
Hacène Khiri ◽  
Victoria Gerolami ◽  
Marc Bourliere ◽  
Jean M. Feryn ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Storage Conditions ◽  
Quantitative Detection ◽  
Hepatitis C Virus Rna ◽  
Virus Rna ◽  
And Storage
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