REPLY TO HEP‐21‐2202: Analysis and limits of detection of biliary flux

Hepatology ◽  
2021 ◽  
Author(s):  
Nachiket Vartak ◽  
Jan G Hengstler
Keyword(s):  
Limits Of Detection

Ion-Associates of N,N-Diethyllysergamide with Some Sulfophthaleins and Azo Dyes

1994 ◽  
Vol 59 (3) ◽  
pp. 575-581 ◽  
Author(s):  
Zdeněk Skaličan ◽  
Zbyněk Kobliha ◽  
Emil Halámek
Keyword(s):  
Azo Dyes ◽  
Limits Of Detection ◽  
Extraction Constants ◽  
Cresol Red ◽  
Extraction Spectrophotometry

Ion-associates of N,N-diethyllysergamide with the sulfophthaleins: Bromoxylenol Blue, Eriochrome Cyanine R, Xylenol Blue, and Cresol Red, and the azo dyes: Acid Black I and Orange-I were studied by extraction spectrophotometry. The extraction recoveries, distribution ratios, conditional extraction constants and limits of detection and determination were calculated.

scholarly journals Erratum: Principles for Sensitive and Robust Biomolecular Interaction Analysis: The Limits of Detection and Resolution of Diffraction-Limited Focal Molography [Phys. Rev. Applied 11, 014056 (2019)]

2021 ◽  
Vol 15 (6) ◽  
Author(s):  
Andreas Frutiger ◽  
Yves Blickenstorfer ◽  
Silvio Bischof ◽  
Csaba Forró ◽  
Volker Gatterdam ◽  
...  
Keyword(s):  
Interaction Analysis ◽  
Biomolecular Interaction ◽  
Limits Of Detection ◽  
Biomolecular Interaction Analysis

HPLC Analysis of Oxytetracycline Residues in Honey

1986 ◽  
Vol 49 (5) ◽  
pp. 383-388 ◽  
Author(s):  
PETER SPORNS ◽  
SUET KWAN ◽  
LAWRENCE A. ROTH
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Aqueous Solutions ◽  
High Performance ◽  
Hplc Analysis ◽  
Extraction Procedure ◽  
Hplc Method ◽  
Limits Of Detection ◽  
Ph Values ◽  
Double Extraction

Oxytetracycline (OTC), also known commercially as Terramycin, was determined to be more stable in honey than in buffered aqueous solutions at similar pH values and temperatures. A rapid high performance liquid chromatography (HPLC) method was developed to detect and quantitate OTC using a 1:1 dilution (wt/wt) of honey samples in water. Using 355 nm as the wavelength of detection, amounts as low as 0.5 μg/ml could be detected in the above solution. The limits of detection were lowered considerably by a double extraction procedure.

A tensammetric study of polyacrylamides, including their determination

1988 ◽  
Vol 66 (10) ◽  
pp. 2658-2663 ◽  
Author(s):  
Truis Smith-Palmer ◽  
Byron R. Wentzell ◽  
John C. Donini ◽  
Robert J. Jerrard
Keyword(s):  
Aqueous Solutions ◽  
Limits Of Detection ◽  
Dilute Aqueous Solutions ◽  
Phase Sensitive

Phase-sensitive tensammetry is shown to be a convenient and useful way to analyse polyacrylamides and associated derivatives (pams) in dilute aqueous solutions. Standard curves were obtained with ranges varying from 9 to 25 ppm and limits of detection varying from 0.7 to 2 ppm. Ways to change the range and sensitivity are discussed and demonstrated. Typical interferences and their effects are tabulated, and the analysis of pams in coal washings is discussed. The analysis of certain mixtures of polyacrylamides is shown to be possible.

Challenging the limits of detection of sialylated Thomsen-Friedenreich antigens by in-gel deglycosylation and nano-LC-MALDI-TOF-MS

2013 ◽  
Vol 34 (16) ◽  
pp. 2337-2341 ◽  
Author(s):  
Andreia Almeida ◽  
José A. Ferreira ◽  
Filipe Teixeira ◽  
Catarina Gomes ◽  
M. Natália D. S. Cordeiro ◽  
...  
Keyword(s):  
Limits Of Detection ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Tof Ms

Simultaneous quantification of vitamin E and vitamin E metabolites in equine plasma and serum using LC-MS/MS

2021 ◽  
pp. 104063872110054
Author(s):  
Hadi Habib ◽  
Carrie J. Finno ◽  
Ingrid Gennity ◽  
Gianna Favro ◽  
Erin Hales ◽  
...  
Keyword(s):  
Vitamin E ◽  
Normal Growth ◽  
Alpha Tocopherol ◽  
Limits Of Detection ◽  
Phase Gradient ◽  
Equine Plasma ◽  
Accuracy And Precision ◽  
Low Concentrations ◽  
Liquid Chromatography Tandem Mass ◽  
Acquity Uplc

Vitamin E deficiencies can impact normal growth and development in humans and animals, and assessment of circulating levels of vitamin E and its metabolites may be an important endpoint for evaluation. Development of a sensitive method to detect and quantify low concentrations of vitamin E and metabolites in biological specimens allows for a proper diagnosis for patients and animals that are deficient. We developed a method to simultaneously extract, detect, and quantify the vitamin E compounds alpha-tocopherol (α-TP), gamma-tocopherol (γ-TP), alpha-tocotrienol (α-TT), and gamma-tocotrienol (γ-TT), and the corresponding metabolites formed after β-oxidation of α-TP and γ-TP, alpha-carboxymethylbutyl hydroxychroman (α-CMBHC) and alpha- or gamma-carboxyethyl hydroxychroman (α- or γ-CEHC), respectively, from equine plasma and serum. Quantification was achieved through liquid chromatography–tandem mass spectrometry. We applied a 96-well high-throughput format using a Phenomenex Phree plate to analyze plasma and serum. Compounds were separated by using a Waters ACQUITY UPLC BEH C18 column with a reverse-phase gradient. The limits of detection for the metabolites and vitamin E compounds were 8–330 pg/mL. To validate the method, intra-day and inter-day accuracy and precision were evaluated along with limits of detection and quantification. The method was then applied to determine concentrations of these analytes in plasma and serum of horses. Alpha-TP levels were 3–6 µg/mL of matrix; the metabolites were found at much lower levels, 0.2–1.0 ng/mL of matrix.

An Industry Survey of Current Lower Limits of Detection for Various Radionuclides

1995 ◽  
Vol 69 (1) ◽  
pp. 121-129 ◽  
Author(s):  
F. M. Cox ◽  
C. F. Guenther
Keyword(s):  
Limits Of Detection ◽  
Industry Survey ◽  
Lower Limits

Investigation of Potassium Chloride for Euthanasia of Anesthetized Marine Toads (Rhinella marina)

2021 ◽  
Author(s):  
Meghan M. Louis ◽  
Gregory Scott ◽  
Dustin Smith ◽  
Brigid V. Troan ◽  
Larry J. Minter ◽  
...  
Keyword(s):  
Potassium Chloride ◽  
Potassium Concentration ◽  
Plasma Potassium ◽  
Post Treatment ◽  
Spontaneous Movement ◽  
Limits Of Detection ◽  
Rhinella Marina ◽  
Loss Of Righting Reflex ◽  
Righting Reflex ◽  
Technique Failure

Euthanasia techniques in amphibians are poorly described and sparsely validated. This study investigated potassium chloride (KCl) for euthanasia of anesthetized marine toads ( Rhinella marina ). Twenty three toads were immersed in buffered MS-222 (2 g/L) for five minutes (min) beyond loss of righting reflex, manually removed, and randomly administered KCl (n = 6/group) via one of three routes: intracardiac at 10 mEq/kg (IC), intracoelomic at 100 mEq/kg (ICe), or immersion at 4500 mEq/L (IMS) or no treatment (C) (n = 5/group). Doppler sounds were assessed continuously from prior to treatment until two min post-treatment and every five min thereafter until sound cessation or resumption of spontaneous movement. Plasma potassium concentration (K+) was measured at the time of Doppler sound cessation in ICe and IMS. In IC, ICe, IMS, and C, Doppler sound cessation occurred in 4/6, 6/6, 6/6, and 1/5 toads with median (range) or mean + SD times of 0.23 (0-4.65), 17.5 + 9.0, 40.6 + 10.9, and >420 min, respectively. Nonsuccess in 2/6 toads in IC was suspected due to technique failure. Plasma K+ exceeded the limits of detection (>9 mmol/L) in 12/12 toads in ICe and IMS. Five of six toads in C resumed spontaneous movement at median (range) times of 327 (300-367) min. KCl delivered via an intracardiac, intracoelomic, or immersion routes resulted in Doppler sound cessation in 16 of 18 toads and may be appropriate for euthanasia of anesthetized marine toads.

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