Cellular reactions against Burkitt lymphoma cells. I. Colony inhibition with effector cells from patients with Burkitt's lymphoma

Burkitt’s lymphoma is a highly aggressive type of non-Hodgkin’s lymphoma, linked to the Epstein-Barr virus, which induces oxidative stress and DNA damage in the infected cells. We investigated the cytotoxicity and redox-modulating ability of ethyl acetate (EtOAc) and n-butanol (n-BuOH) extracts from Geum urbanum L. roots and aerial parts on Burkitt`s lymphoma cells (BLC), to elucidate their impact on oxidative stress and cell survival. BLC Raji was treated with EtOAc and n-BuOH extracts to analyze: cell viability; induction of apoptosis; hydroperoxides and reactive nitrogen species (RNS) by 2’,7’-dichlorodihydrofluorescein assay; superoxide by dihydroethidium assay; total antioxidant capacity by TAC assay. All extracts suppressed cell growth and induce apoptosis. n-BuOH extracts possessed higher cytotoxicity and pro-apoptotic activity compared to EtOAc. The fractions decreased the hydroperoxides and RNS levels. There was no correlation between the DCF fluorescence in the treated cells and their viability (R = -0.3722; p > 0.05). Root extracts decreased the superoxide level, while the leaf extracts did not. There was a good correlation between the dihydroethidium fluorescence in the treated cells and their viability (R = 0.9843; p < 0.01). All extracts increased the TAC of BLC. G. urbanum extracts serve as redox-modulators and anti-inflammatory compounds, decreasing the intracellular level of “oncogenic” superoxide and cell proliferation.

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Cell Phenotype Dependent Down-regulation of MHC Class I Antigens in Burkitt’s Lymphoma Cells

Current Topics in Microbiology and Immunology - Mechanisms in B-Cell Neoplasia 1990 ◽

10.1007/978-3-642-75889-8_38 ◽

1990 ◽

pp. 309-316 ◽

Cited By ~ 1

Author(s):

M. G. Masucci

Keyword(s):

Mhc Class I ◽

Burkitt’S Lymphoma ◽

Burkitt's Lymphoma ◽

Class I ◽

Cell Phenotype ◽

Lymphoma Cells ◽

Down Regulation ◽

Mhc Class I Antigens ◽

Burkitt’S Lymphoma Cells

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The interaction between Vav1 and EBNA1 promotes survival of Burkitt's lymphoma cells by down-regulating the expression of Bim

Biochemical and Biophysical Research Communications ◽

10.1016/j.bbrc.2019.02.108 ◽

2019 ◽

Vol 511 (4) ◽

pp. 787-793 ◽

Cited By ~ 1

Author(s):

Ruikun Wang ◽

Jiyan Wang ◽

Nianchao Zhang ◽

Yajuan Wan ◽

Yaohui Liu ◽

...

Keyword(s):

Burkitt’S Lymphoma ◽

Burkitt's Lymphoma ◽

Lymphoma Cells ◽

Burkitt’S Lymphoma Cells

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Elevated NAD(P) glycohydrolase activity: a possible enzymatic marker for malignancy in Burkitt's lymphoma cells

Blood ◽

10.1182/blood.v60.4.912.912 ◽

1982 ◽

Vol 60 (4) ◽

pp. 912-917 ◽

Cited By ~ 8

Author(s):

H Skala ◽

GM Lenoir ◽

AL Pichard ◽

M Vuillaume ◽

JC Dreyfus

Keyword(s):

Cell Lines ◽

Burkitt’S Lymphoma ◽

Chromosome 8 ◽

Burkitt's Lymphoma ◽

Activity Levels ◽

Lymphoma Cells ◽

Cell Extracts ◽

Enzymatic Marker ◽

The Difference ◽

Burkitt’S Lymphoma Cells

Abstract A comparative analysis of enzymatic activities has been performed on 47 human continuous lymphoid lines: 22 tumors derived from Burkitt's lymphoma lines, 6 other lymphomatous long-term cultures, and 19 nonmalignant ties determined on the cell extracts. 4 showed no significant differences between the various lines. They included adenosine diphosphoribose incorporation, glucose-6-phosphate dehydrogenase, cyclic-AMP phosphodiesterase, and glutathione reductase. However, striking differences of activity were found for the enzyme, NAD(P) glycohydrolase (EC 3.2.2.6). Activity levels were, as a mean, four times higher in Burkitt's lymphoma-derived cell lines than in nonmalignant control lines, and the difference was highly significant (p less than 0.02). All Burkitt cell lines containing translocations of chromosome 8 with either chromosomes 2, 14 or 22 showed an increased activity. The specificity and significance of this possible enzymatic marker of Burkitt's lymphoma cells is discussed.

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