scholarly journals Alpha-amanitin resistant transcription of protein coding genes in insect and bloodstream form Trypanosoma brucei.

1989 ◽  
Vol 8 (13) ◽  
pp. 4259-4263 ◽  
Author(s):  
G. Rudenko ◽  
D. Bishop ◽  
K. Gottesdiener ◽  
L.H. Van der Ploeg
Keyword(s):  
Trypanosoma Brucei ◽  
Bloodstream Form ◽  
Protein Coding ◽  
Protein Coding Genes ◽  
Alpha Amanitin

The spliced leader sequence of Trypanosoma brucei has a potential role as a cap donor structure

1985 ◽  
Vol 5 (9) ◽  
pp. 2487-2490
Author(s):  
M J Lenardo ◽  
D M Dorfman ◽  
J E Donelson
Keyword(s):  
Trypanosoma Brucei ◽  
Potential Role ◽  
Leader Sequence ◽  
The Body ◽  
Trypanosoma Brucei Brucei ◽  
Protein Coding ◽  
Spliced Leader ◽  
Protein Coding Genes ◽  
Separate Locus

Trypanosoma brucei brucei and other trypanosomatid species are unique among eucaryotes because transcription of their protein-coding genes is discontinuous. The 5' ends of their mRNAs consist of an identical 35-nucleotide spliced leader which is encoded at a separate locus from that for the body of the protein-coding transcript. We show here that the spliced leader transcript contains a 5' cap structure and suggest that at least one function of the spliced leader sequence is to provide a cap structure to trypanosome mRNAs.

scholarly journals Functional Characterization of a Trypanosoma brucei TATA-Binding Protein-Related Factor Points to a Universal Regulator of Transcription in Trypanosomes

2004 ◽  
Vol 24 (21) ◽  
pp. 9610-9618 ◽  
Author(s):  
Jia-peng Ruan ◽  
George K. Arhin ◽  
Elisabetta Ullu ◽  
Christian Tschudi
Keyword(s):  
Trypanosoma Brucei ◽  
Binding Protein ◽  
Functional Characterization ◽  
Tata Binding Protein ◽  
Related Factor ◽  
Protein Coding ◽  
Pol Ii ◽  
Protein Coding Genes ◽  
Pol I ◽  
Rna Genes

ABSTRACT Transcriptional mechanisms remain poorly understood in trypanosomatid protozoa. In particular, there is no knowledge about the function of basal transcription factors, and there is an apparent rarity of promoters for protein-coding genes transcribed by RNA polymerase (Pol) II. Here we describe a Trypanosoma brucei factor related to the TATA-binding protein (TBP). Although this TBP-related factor (TBP-related factor 4 [TRF4]) has about 31% identity to the TBP core domain, several key residues involved in TATA box binding are not conserved. Depletion of the T. brucei TRF4 (TbTRF4) by RNA interference revealed an essential role in RNA Pol I, II, and III transcription. Using chromatin immunoprecipitation, we further showed that TRF4 is recruited to the Pol I-transcribed procyclic acidic repetitive genes, Pol II-transcribed spliced leader RNA genes, and Pol III-transcribed U-snRNA and 7SL RNA genes, thus supporting a role for TbTRF4 in transcription performed by all three nuclear RNA polymerases. Finally, a search for TRF4 binding sites in the T. brucei genome led to the identification of such sites in the 3′ portion of certain protein-coding genes, indicating a unique aspect of Pol II transcription in these organisms.

scholarly journals LncRNAs Are Differentially Expressed between Wildtype and Cell Line Strains of African Trypanosomes

2022 ◽  
Vol 8 (1) ◽  
pp. 7
Author(s):  
Hyung Chul Kim ◽  
Emmitt R. Jolly
Keyword(s):  
Gene Expression ◽  
Trypanosoma Brucei ◽  
Cell Lines ◽  
Differentially Expressed ◽  
Protein Coding ◽  
African Trypanosomes ◽  
Transcriptomic Data ◽  
Protein Coding Genes ◽  
Parasitic Protist ◽  
Non Coding Rnas

Trypanosoma brucei is a parasitic protist that causes African sleeping sickness. The establishment of T. brucei cell lines has provided a significant advantage for the majority of T. brucei research. However, these cell lines were isolated and maintained in culture for decades, occasionally accumulating changes in gene expression. Since trypanosome strains have been maintained in culture for decades, it is possible that difference may have accumulated in fast-evolving non-coding RNAs between trypanosomes from the wild and those maintained extensively in cultures. To address this, we compared the lncRNA expression profile of trypanosomes maintained as cultured cell lines (CL) to those extracted from human patients, wildtype (WT). We identified lncRNAs from CL and WT from available transcriptomic data and demonstrate that CL and WT have unique sets of lncRNAs expressed. We further demonstrate that the unique and shared lncRNAs are differentially expressed between CL and WT parasites, and that these lncRNAs are more evenly up-regulated and down-regulated than protein-coding genes. We validated the expression of these lncRNAs using qPCR. Taken together, this study demonstrates that lncRNAs are differentially expressed between cell lines and wildtype T. brucei and provides evidence for potential evolution of lncRNAs, specifically in T. brucei maintained in culture.

scholarly journals The evolution of pathogenic trypanosomes

1999 ◽  
Vol 15 (4) ◽  
pp. 673-684 ◽  
Author(s):  
Jamie R. Stevens ◽  
Wendy C. Gibson
Keyword(s):  
Trypanosoma Brucei ◽  
Ribosomal Rna ◽  
Fossil Record ◽  
Phylogenetic Trees ◽  
Recent Progress ◽  
Molecular Data ◽  
Protein Coding ◽  
Protein Coding Genes ◽  
History Of ◽  
Vertebrate Hosts

In the absence of a fossil record, the evolution of protozoa has until recently largely remained a matter for speculation. However, advances in molecular methods and phylogenetic analysis are now allowing interpretation of the "history written in the genes". This review focuses on recent progress in reconstruction of trypanosome phylogeny based on molecular data from ribosomal RNA, the miniexon and protein-coding genes. Sufficient data have now been gathered to demonstrate unequivocally that trypanosomes are monophyletic; the phylogenetic trees derived can serve as a framework to reinterpret the biology, taxonomy and present day distribution of trypanosome species, providing insights into the coevolution of trypanosomes with their vertebrate hosts and vectors. Different methods of dating the divergence of trypanosome lineages give rise to radically different evolutionary scenarios and these are reviewed. In particular, the use of one such biogeographically based approach provides new insights into the coevolution of the pathogens, Trypanosoma brucei and Trypanosoma cruzi, with their human hosts and the history of the diseases with which they are associated.

scholarly journals RNA polymerase I can mediate expression of CAT and neo protein-coding genes in Trypanosoma brucei.

1991 ◽  
Vol 10 (11) ◽  
pp. 3387-3397 ◽  
Author(s):  
G. Rudenko ◽  
H.M. Chung ◽  
V.P. Pham ◽  
L.H. Van der Ploeg
Keyword(s):  
Rna Polymerase ◽  
Trypanosoma Brucei ◽  
Rna Polymerase I ◽  
Protein Coding ◽  
Protein Coding Genes ◽  
Polymerase I

scholarly journals The spliced leader sequence of Trypanosoma brucei has a potential role as a cap donor structure.

1985 ◽  
Vol 5 (9) ◽  
pp. 2487-2490 ◽  
Author(s):  
M J Lenardo ◽  
D M Dorfman ◽  
J E Donelson
Keyword(s):  
Trypanosoma Brucei ◽  
Potential Role ◽  
Leader Sequence ◽  
The Body ◽  
Trypanosoma Brucei Brucei ◽  
Protein Coding ◽  
Spliced Leader ◽  
Protein Coding Genes ◽  
Separate Locus

Trypanosoma brucei brucei and other trypanosomatid species are unique among eucaryotes because transcription of their protein-coding genes is discontinuous. The 5' ends of their mRNAs consist of an identical 35-nucleotide spliced leader which is encoded at a separate locus from that for the body of the protein-coding transcript. We show here that the spliced leader transcript contains a 5' cap structure and suggest that at least one function of the spliced leader sequence is to provide a cap structure to trypanosome mRNAs.

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