scholarly journals Comparison of Antioxidant Activity of Barley (Hordeum vulgare L.) and Malt Extracts with the Content of Free Phenolic Compounds Measured by High Performance Liquid Chromatography Coupled with CoulArray Detector

2008 ◽  
Vol 114 (2) ◽  
pp. 150-159 ◽  
Author(s):  
Markéta Dvořáková ◽  
Maëlle Douanier ◽  
Marie Jurková ◽  
Vladimír Kellner ◽  
Pavel Dostálek
Keyword(s):  
Antioxidant Activity ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Phenolic Compounds ◽  
Hordeum Vulgare ◽  
High Performance ◽  
Hordeum Vulgare L

Application of denaturing high-performance liquid chromatography for mapping of single nucleotide polymorphisms in barley (Hordeum vulgare L.)

Genome ◽  
2001 ◽  
Vol 44 (4) ◽  
pp. 523-528 ◽  
Author(s):  
Raja Kota ◽  
Markus Wolf ◽  
Wolfgang Michalek ◽  
Andreas Graner
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Hordeum Vulgare ◽  
Dna Sequence ◽  
High Throughput ◽  
Doubled Haploid ◽  
High Performance ◽  
Nucleotide Polymorphisms ◽  
Hordeum Vulgare L ◽  
Single Nucleotide

Recent advances in DNA sequence analysis and the establishment of high-throughput assays have provided the framework for large-scale discovery and analysis of DNA sequence variation. In this context, single nucleotide polymorphisms (SNPs) are of particular interest. To initiate a systematic approach to develop an SNP map of barley (Hordeum vulgare L.), we have employed denaturing high-performance liquid chromatography (DHPLC) to analyse segregating SNP patterns in a doubled-haploid (DH) mapping population. To this end, SNPs between the parental genotypes were identified using a direct sequencing approach. Once a SNP was established between the parents, the optimal melting temperature of the PCR fragment containing the SNP was predicted for its analysis by DHPLC. Following the detection of the optimal temperature, the DH lines were analysed for the presence of either of the alleles. To test the utility of the analysis, data from previously mapped RFLP markers from which these SNPs were derived were compared. Results from these experiments indicate that DHPLC can be efficiently employed in analysing SNPs on a high-throughput scale.Key words: denaturing high performance liquid chromatography, doubled-haploid lines, restriction fragment length polymorphism, genetic mapping, molecular markers.

scholarly journals The study of phenolic compounds and antioxidant activity of raw materials of Heliopsis helianthoides (l.) sweet

2021 ◽  
Vol 34 (1) ◽  
pp. 28-33
Author(s):  
Mariia Pavlenko-Badnaoui ◽  
Viktoriia Protska ◽  
Nadiia Burda ◽  
Iryna Zhuravel ◽  
Viktoriia Kuznetsova
Keyword(s):  
Antioxidant Activity ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Chemical Composition ◽  
Phenolic Compounds ◽  
High Performance ◽  
Raw Materials ◽  
Hydroxycinnamic Acid ◽  
Bioactive Substances

Abstract Using high performance liquid chromatography (HPLC), we studied the chemical composition and antioxidant activity of bioactive substances in the roots, leaves, flowers and seeds of Heliopsis helianthoides. The results of our study showed the presence of 5 phenolic compounds in its roots, 4 phenolic compounds in its leaves, 10 phenolic compounds in its flowers and 8 phenolic compounds in its seeds. The highest content of identified compounds was found in the leaves of this plant – 3192.20±79.78 mg/kg. The dominating hydroxycinnamic acid was chlorogenic acid. This had its highest concentration (1537.21±38.43 mg/kg) in the Heliopsis helianthoides leaves. Among flavonoids, luteolin prevailed in the roots, apigenin-7-glucoside prevailed in the seeds and rutin prevailed in the leaves and flowers. Maximum rutin content (1426.64±35.67 mg/kg) was found in the Heliopsis helianthoides leaves. Antioxidant activity study in vitro uncovered the substantial antioxidant potential of bioactive substances (BASs) in all tested samples of the raw materials, being within the limits of 2.81-8.13 mg/g. Most active in this respect were Heliopsis helianthoides leaves. The obtained data indicate the feasibility of the development of new antioxidant active drugs on the basis of raw materials of Heliopsis helianthoides.

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