Six‐transmembrane epithelial antigen of the prostate 1 is associated with tumor invasion and migration in endometrial carcinomas

Abstract Background: Hepatocellular carcinoma (HCC) is one of the leading causes of cancer-related deaths worldwide. Recent evidence indicates that circular RNAs (circRNAs) play important roles in tissue development, gene regulation, and carcinogenesis. However, whether circRNAs are involved in HCC progression and encode functional proteins remains largely unknown.Methods: Circular RNA microarrays were performed using three pathologically diagnosed HCC samples and their paired adjacent normal liver tissues. Cell invasion, migration, cell cycle, and apoptosis after circRNA overexpression were measured using a transwell culture system, a wound healing assay, and flow cytometry . Full-length, mutated, and truncated sequences of circEPS15 with a FLAG tag were inserted inside a circular expression vector. Western blotting was used to confirm circEPS15 expression and the requirement of internal ribosomal entry site (IRES) elements within the circRNA. The miRNA and mRNA expression profiles were obtained by analyzing data retrieved from The Cancer Genome Atlas (TCGA) database. We then constructed a ceRNA network of mRNAs, miRNAs, and circEPS15. Using tissue samples from own patients, we also verified certain analytical results with quantitative real-time PCR (qRT-PCR).Results: The expression of circEPS15 was downregulated in HCC tissues, and the survival curves showed that low circEPS15 levels were associated with poor overall survival in HCC patients. Overexpression of circEPS15 suppressed tumor invasion and migration by inhibiting the TJP1/CDH2/VIM signaling pathway and retarded cell cycle progression, but it had no effect on cell apoptosis. ceRNA analysis and qRT-PCR showed that there might be a circRNA (circEPS15)-miRNA (miR-24-3p)-mRNA (CIDEA) network in HCC. The spanning junction open reading frame in circEPS15 driven by IRES encoded a novel protein.Conclusions: Endogenous circEPS15 plays a novel role in repressing HCC through the ceRNA network and encoding a functional protein.

Download Full-text

Exosomic miR-224 regulated tumor invasion and migration through IL-6/STAT3 pathway in hepatocellular carcinoma

10.26226/morressier.59a6b341d462b80290b53ec7 ◽

2017 ◽

Author(s):

fangmei an

Keyword(s):

Hepatocellular Carcinoma ◽

Tumor Invasion ◽

Stat3 Pathway ◽

Invasion And Migration ◽

And Migration

Download Full-text

Non-small cell lung cancer: miR-30d suppresses tumor invasion and migration by directly targeting NFIB

Biotechnology Letters ◽

10.1007/s10529-017-2428-9 ◽

2017 ◽

Vol 39 (12) ◽

pp. 1827-1834 ◽

Cited By ~ 3

Author(s):

Yubing Wu ◽

Jingnan Zhang ◽

Shizhen Hou ◽

Ziming Cheng ◽

Maoxi Yuan

Keyword(s):

Lung Cancer ◽

Small Cell Lung Cancer ◽

Cell Lung Cancer ◽

Tumor Invasion ◽

Small Cell ◽

Small Cell Lung ◽

Invasion And Migration ◽

And Migration

Download Full-text

GALNT14 mediates tumor invasion and migration in breast cancer cell MCF-7

Molecular Carcinogenesis ◽

10.1002/mc.22186 ◽

2014 ◽

Vol 54 (10) ◽

pp. 1159-1171 ◽

Cited By ~ 29

Author(s):

Tian Huanna ◽

Zuo Tao ◽

Wang Xiangfei ◽

An Longfei ◽

Xie Yuanyuan ◽

...

Keyword(s):

Breast Cancer ◽

Cancer Cell ◽

Breast Cancer Cell ◽

Tumor Invasion ◽

Invasion And Migration ◽

And Migration ◽

Mcf 7

Download Full-text

Transcriptional regulation of adrenomedullin by oncostatin M in human astroglioma cells: Implications for tumor invasion and migration

Scientific Reports ◽

10.1038/srep06444 ◽

2014 ◽

Vol 4 (1) ◽

Cited By ~ 8

Author(s):

Seul Ye Lim ◽

So-Hee Ahn ◽

Hyunju Park ◽

Jungsul Lee ◽

Kyungsun Choi ◽

...

Keyword(s):

Transcriptional Regulation ◽

Tumor Invasion ◽

Oncostatin M ◽

Invasion And Migration ◽

And Migration ◽

Astroglioma Cells

Download Full-text

Inhibition of tumor–microenvironment interaction and tumor invasion by small-molecule allosteric inhibitor of DDR2 extracellular domain

Proceedings of the National Academy of Sciences ◽

10.1073/pnas.1805020115 ◽

2018 ◽

Vol 115 (33) ◽

pp. E7786-E7794 ◽

Cited By ~ 30

Author(s):

Whitney R. Grither ◽

Gregory D. Longmore

Keyword(s):

Small Molecule ◽

Tumor Invasion ◽

Cancer Metastasis ◽

Metastatic Breast ◽

Extracellular Domain ◽

Breast Cancer Metastasis ◽

Invasion And Migration ◽

Molecule Inhibitor ◽

Ligand Interactions ◽

And Migration

The action of the collagen binding receptor tyrosine kinase (RTK) discoidin domain receptor 2 (DDR2) in both tumor and tumor stromal cells has been established as critical for breast cancer metastasis. Small molecule inhibitors that target the extracellular domain of RTKs are rare, as they have classically been regarded as too small to block binding with large polypeptide ligands. Here, we report the identification and characterization of a selective, extracellularly acting small molecule inhibitor (WRG-28) of DDR2 that uniquely inhibits receptor–ligand interactions via allosteric modulation of the receptor. By targeting DDR2, WRG-28 inhibits tumor invasion and migration, as well as tumor-supporting roles of the stroma, and inhibits metastatic breast tumor cell colonization in the lungs. These findings represent an approach to inhibiting tumor–stromal interactions and support the development of allosteric inhibitors of DDR2, such as WRG-28, as a promising approach to antimetastasis treatment.

Download Full-text

circEPS15 Overexpression in Hepatocellular Carcinoma Modulates Tumor Invasion and Migration

10.21203/rs.3.rs-69340/v1 ◽

2021 ◽

Author(s):

Maolin Tian ◽

Gang Li ◽

Bin Jiang ◽

Abuduhaibaier Sadula ◽

Dianrong Xiu ◽

...

Keyword(s):

Tumor Invasion ◽

Expression Profiles ◽

The Cancer Genome Atlas ◽

Circular Rnas ◽

Functional Protein ◽

Entry Site ◽

Poor Overall Survival ◽

Invasion And Migration ◽

Cerna Network ◽

And Migration

Abstract BackgroundHepatocellular carcinoma (HCC) is one kind of the leading causes of cancer-related deaths in the world. Recent evidence indicates that circular RNAs (circRNAs) play important roles in tissue development, gene transcription, signal regulation and tumorigenesis. However, whether circRNAs are involved in HCC progression and encode functional proteins remains largely unknown.MethodscircRNAs microarrays wereperformed using three pathologically diagnosed HCC samples and their paired adjacent normal liver tissues. Cell invasion, migration, cycle, and apoptosis post circRNA overexpression were measured using a transwell culture system, a wound healing assay, and flow cytometry. Full-length, mutated, and truncated sequences of circEPS15 with a FLAG tag were inserted into a circular expression vector. Western blotting was used to confirm circEPS15 expression and the requirement of internal ribosomal entry site (IRES) elements within the circRNA. The miRNA and mRNA expression profiles were obtained by analyzing data retrieved from The Cancer Genome Atlas (TCGA) database. We then constructed a ceRNA network ofcircEPS15, miRNAs, and mRNAs. ResultsThe expression of circEPS15 was downregulated in HCC tissues, and the survival curves showed that low circEPS15 levels were associated with poor overall survival in HCC patients. Overexpression of circEPS15 suppressed tumor invasion and migration by inhibiting the TJP1/CDH2/VIM signaling pathway and retarded cell cycle progression, yet had no effect on cell apoptosis. ceRNA analysis and qRT-PCR results suggest a possible circRNA (circEPS15)-miRNA (miR-24-3p)-mRNA (CIDEA) network in HCC. The spanning junction open reading frame in circEPS15 driven by IRES encoded a novel protein.ConclusionsEndogenous circEPS15 plays a novel role in repressing HCC through the ceRNA network and encoding a functional protein.

Download Full-text