The interplay between glioblastoma and microglia cells leads to endothelial cell monolayer dysfunction via the interleukin‐6‐induced JAK2/STAT3 pathway

2019 ◽  
Vol 234 (11) ◽  
pp. 19750-19760 ◽  
Author(s):  
Marina Couto ◽  
Vanessa Coelho‐Santos ◽  
Liliana Santos ◽  
Carlos Fontes‐Ribeiro ◽  
Ana Paula Silva ◽  
...  
Keyword(s):  
Endothelial Cell ◽  
Interleukin 6 ◽  
Cell Monolayer ◽  
Endothelial Cell Monolayer ◽  
Stat3 Pathway

Arthrospira platensis accelerates the formation of an endothelial cell monolayer and protects against endothelial cell detachment after bacterial contamination

2021 ◽  
pp. 1-11
Author(s):  
A. Krüger-Genge ◽  
S. Steinbrecht ◽  
C.G.H. Jung ◽  
Sophia Westphal ◽  
Stefanie Klöpzig ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Endothelial Cell ◽  
Bacterial Contamination ◽  
Cell Monolayer ◽  
Arthrospira Platensis ◽  
Bacterial Toxin ◽  
Cell Detachment ◽  
Endothelial Cell Monolayer ◽  
Positive Effects ◽  
Anti Oxidant

Within the last years a comprehensive number of scientific studies demonstrated beneficial effect of Arthropira platensis (AP) as dietary supplement due to a high content of proteins, minerals and vitamins. Positive effects like promoting the immune system, reducing inflammation and an anti-oxidant capacity are reported. In this study, the effect of an aqueous AP extract on primary human venous endothelial cells (HUVEC) was investigated. In addition, the effect of AP on HUVEC treated with a bacterial toxin (lipopolysaccharide, LPA), inducing an activation of HUVEC and cellular detachment, was analyzed. Depending on the concentration of AP extract a significantly accelerated formation of an endothelial cell monolayer was observed. Furthermore, the detachment of HUVEC after LPA addition was dramatically reduced by AP. In conclusion, the data are promising and indicatory for an application of Arthrospira platensis in the clinical field.

Effect of Shear Stress on Platelet Adhesion to Expanded Polytetrafluoroethylene, a Silicone Sheet, and an Endothelial Cell Monolayer

ASAIO Journal ◽  
2000 ◽  
Vol 46 (6) ◽  
pp. 696-701 ◽  
Author(s):  
Katsuko Sakai Furukawa ◽  
Takashi Ushida ◽  
Hirohito Sugano ◽  
Tamotsu Tamaki ◽  
Norio Ohshima ◽  
...  
Keyword(s):  
Shear Stress ◽  
Endothelial Cell ◽  
Platelet Adhesion ◽  
Cell Monolayer ◽  
Endothelial Cell Monolayer ◽  
Expanded Polytetrafluoroethylene ◽  
Silicone Sheet

Astroglial cells inhibit the increasing permeability of brain endothelial cell monolayer following hypoxia/reoxygenation

1996 ◽  
Vol 208 (2) ◽  
pp. 101-104 ◽  
Author(s):  
Takeo Kondo ◽  
Hiroyuki Kinouchi ◽  
Makoto Kawase ◽  
Takashi Yoshimoto
Keyword(s):  
Endothelial Cell ◽  
Cell Monolayer ◽  
Brain Endothelial Cell ◽  
Endothelial Cell Monolayer ◽  
Astroglial Cells ◽  
Hypoxia Reoxygenation

Effects of Rat Hepatocytes on Macromolecular Permeability of Bovine Aortic Endothelial Cell Monolayer

1996 ◽  
Vol 16 (2) ◽  
pp. 105-110 ◽  
Author(s):  
N. Utoguchi ◽  
H. Mizuguchi ◽  
K. Saeki ◽  
K. Ikeda ◽  
S. Nakagawa ◽  
...  
Keyword(s):  
Endothelial Cell ◽  
Cell Monolayer ◽  
Rat Hepatocytes ◽  
Bovine Aortic Endothelial Cell ◽  
Endothelial Cell Monolayer ◽  
Aortic Endothelial Cell ◽  
Macromolecular Permeability ◽  
Aortic Endothelial

Evans blue dye as an indicator of albumin permeability across a brain endothelial cell monolayer in vitro

Neuroreport ◽  
2021 ◽  
Vol 32 (11) ◽  
pp. 957-964
Author(s):  
Meng-Chih Wu ◽  
Jye-Lin Hsu ◽  
Ted Weita Lai
Keyword(s):  
Endothelial Cell ◽  
Evans Blue ◽  
Cell Monolayer ◽  
Blue Dye ◽  
Brain Endothelial Cell ◽  
Endothelial Cell Monolayer ◽  
Evans Blue Dye

CHARACTERIZATION OF DISORDERS OF PLATELET-VESSEL WALL INTERACTION IN AN AGGREGOMETER INCORPORATING BLOOD FLOW PAST AN ENDOTHELIAL CELL MONOLAYER

1987 ◽  
Author(s):  
E F Grabowski ◽  
K McKenny
Keyword(s):  
Endothelial Cell ◽  
Cell Monolayer ◽  
Fold Increase ◽  
Flow Chamber ◽  
Fluorescent Label ◽  
Endothelial Cell Monolayer ◽  
Flowing Blood ◽  
Normal States ◽  
Wall Interaction

Epi-fluorescence videomicroscopy permits real-time imaging of platelet (plt) adhesion-aggregation to a defined microinjury site of an endothelial cell monolayer (ECM) exposed to flowing blood. The fluorescent label is the TAB murine monoclonal antibody (courtesy of Dr. R.P. McEver) directed against human pit cp HB, together with a fluorescein-conjugated goat F(ab')2 against murine immunoglobulin. The combination assures specificity for pit membranes, yet leaves pit function intact. Bovine aortic ECM, grown on rectangular cover glasses, comprise one wall of a flow chamber mounted on a vertical microscope stage. A 6-0 sterile suture, drawn across the ECM in a direction transverse to flow, creates microinjuries of width 70 ± 15 (mean ± SD). Pit deposition is virtually absent upon intact and confluent regions of the ECM. On microinjury sites and at a shear rate of 270 sec-1, however, computer-enhanced images show pit adherence, aggregation, and embolization. Pretreatment of the ECM with 1.0 mMFC lysine acetyl salicylate, further, leads to a three-fold increase in aggregate length. ECM products inhibitable by aspirin, therefore, modulate adhesion-aggregation in disease and normal states under physiologic flow conditions. The Table shows that nercent coverage of the injury area, and mean aggregate length readily discriminate normal, post-aspirin, and von Willebrand's (vWD's) bloods. Aggregate length is reduced in vWD's blood to a greater degree (p<0.01) than by oral aspirin, while the latter is associated with a paradoxic increase (p<0.01) in single plt adhesion.

Sign in / Sign up

Export Citation Format

Share Document