Physiological and biochemical responses of Brassica napus L. to drought‐induced stress by the application of biochar and Plant Growth Promoting Rhizobacteria

2021 ◽  
Author(s):  
Gul Lalay ◽  
Sami Ullah ◽  
Iftikhar Ahmed
Keyword(s):  
Brassica Napus ◽  
Plant Growth ◽  
Plant Growth Promoting Rhizobacteria ◽  
Brassica Napus L ◽  
Biochemical Responses ◽  
Induced Stress ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Physiological And Biochemical

scholarly journals Unraveling Mechanisms and Impact of Microbial Recruitment on Oilseed Rape (Brassica napus L.) and the Rhizosphere Mediated by Plant Growth-Promoting Rhizobacteria

2021 ◽  
Vol 9 (1) ◽  
pp. 161
Author(s):  
Ying Liu ◽  
Jie Gao ◽  
Zhihui Bai ◽  
Shanghua Wu ◽  
Xianglong Li ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Plant Growth ◽  
Community Composition ◽  
Oilseed Rape ◽  
Plant Growth Promoting Rhizobacteria ◽  
16S Rrna Genes ◽  
Rrna Genes ◽  
Brassica Napus L ◽  
Plant Growth Promoting ◽  
Growth Promoting

Plant growth-promoting rhizobacteria (PGPR) are noticeably applied to enhance plant nutrient acquisition and improve plant growth and health. However, limited information is available on the compositional dynamics of rhizobacteria communities with PGPR inoculation. In this study, we investigated the effects of three PGPR strains, Stenotrophomonas rhizophila, Rhodobacter sphaeroides, and Bacillus amyloliquefaciens on the ecophysiological properties of Oilseed rape (Brassica napus L.), rhizosphere, and bulk soil; moreover, we assessed rhizobacterial community composition using high-throughput Illumina sequencing of 16S rRNA genes. Inoculation with S. rhizophila, R. sphaeroides, and B. amyloliquefaciens, significantly increased the plant total N (TN) (p < 0.01) content. R. sphaeroides and B. amyloliquefaciens selectively enhanced the growth of Pseudomonadacea and Flavobacteriaceae, whereas S. rhizophila could recruit diazotrophic rhizobacteria, members of Cyanobacteria and Actinobacteria, whose abundance was positively correlated with inoculation, and improved the transformation of organic nitrogen into inorganic nitrogen through the promotion of ammonification. Initial colonization by PGPR in the rhizosphere affected the rhizobacterial community composition throughout the plant life cycle. Network analysis indicated that PGPR had species-dependent effects on niche competition in the rhizosphere. These results provide a better understanding of PGPR-plant-rhizobacteria interactions, which is necessary to develop the application of PGPR.

Antifungal potential against Sclerotinia sclerotiorum (Lib.) de Bary and plant growth promoting abilities of Bacillus isolates from canola (Brassica napus L.) roots

2021 ◽  
pp. 126754
Author(s):  
Igor Daniel Alves Ribeiro ◽  
Evelise Bach ◽  
Fernanda da Silva Moreira ◽  
Aline Reis Müller ◽  
Caroline Pinto Rangel ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Plant Growth ◽  
Sclerotinia Sclerotiorum ◽  
Brassica Napus L ◽  
Antifungal Potential ◽  
Plant Growth Promoting ◽  
Growth Promoting

scholarly journals Selection of the Root Endophyte Pseudomonas brassicacearum CDVBN10 as Plant Growth Promoter for Brassica napus L. Crops

Agronomy ◽  
2020 ◽  
Vol 10 (11) ◽  
pp. 1788
Author(s):  
Alejandro Jiménez-Gómez ◽  
Zaki Saati-Santamaría ◽  
Martin Kostovcik ◽  
Raúl Rivas ◽  
Encarna Velázquez ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Plant Growth ◽  
Dry Weight ◽  
Bacterial Endophytes ◽  
Chemical Fertilizers ◽  
Brassica Napus L ◽  
Bacterial Microbiome ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Pseudomonas Brassicacearum

Rapeseed (Brassica napus L.) is an important crop worldwide, due to its multiple uses, such as a human food, animal feed and a bioenergetic crop. Traditionally, its cultivation is based on the use of chemical fertilizers, known to lead to several negative effects on human health and the environment. Plant growth-promoting bacteria may be used to reduce the need for chemical fertilizers, but efficient bacteria in controlled conditions frequently fail when applied to the fields. Bacterial endophytes, protected from the rhizospheric competitors and extreme environmental conditions, could overcome those problems and successfully promote the crops under field conditions. Here, we present a screening process among rapeseed bacterial endophytes to search for an efficient bacterial strain, which could be developed as an inoculant to biofertilize rapeseed crops. Based on in vitro, in planta, and in silico tests, we selected the strain Pseudomonas brassicacearum CDVBN10 as a promising candidate; this strain produces siderophores, solubilizes P, synthesizes cellulose and promotes plant height in 5 and 15 days-post-inoculation seedlings. The inoculation of strain CDVBN10 in a field trial with no addition of fertilizers showed significant improvements in pod numbers, pod dry weight and shoot dry weight. In addition, metagenome analysis of root endophytic bacterial communities of plants from this field trial indicated no alteration of the plant root bacterial microbiome; considering that the root microbiome plays an important role in plant fitness and development, we suggest this maintenance of the plant and its bacterial microbiome homeostasis as a positive result. Thus, Pseudomonas brassicacearum CDVBN10 seems to be a good biofertilizer to improve canola crops with no addition of chemical fertilizers; this the first study in which a plant growth-promoting (PGP) inoculant specifically designed for rapeseed crops significantly improves this crop’s yields in field conditions.

scholarly journals Assessment of the Capacity of Beneficial Bacterial Inoculants to Enhance Canola (Brassica napus L.) Growth under Low Water Activity

Agronomy ◽  
2020 ◽  
Vol 10 (9) ◽  
pp. 1449
Author(s):  
Dasun Premachandra ◽  
Lee Hudek ◽  
Aydin Enez ◽  
Ross Ballard ◽  
Steve Barnett ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Plant Growth ◽  
Water Activity ◽  
Production Costs ◽  
Annual Rainfall ◽  
Shoot Biomass ◽  
Brassica Napus L ◽  
Environmentally Sustainable ◽  
Plant Growth Promoting ◽  
Growth Promoting

Canola (Brassica napus L.) is the third largest crop produced in Australia after wheat and barley. For such crops, the variability of water access, reduced long-term annual rainfall and increasing water prices, higher overall production costs, and variability in production quantity and quality are driving the exploration of new tools to maintain production in an economical and environmentally sustainable way. Microorganisms associated with the rhizosphere have been shown to enhance plant growth and offer a potential way to maintain or even increase crop production quality and yield in an environmentally sustainable way. Here, seven bacterial isolates from canola rhizosphere samples are shown to enhance canola growth, particularly in low water activity systems. The seven strains all possessed commonly described plant growth promoting traits, including the ability to produce indole-3-acetic acid and 1-aminocyclopropane-1-carboxylate deaminase, and the capacity to solubilise nutrients (Fe2+/3+ and PO43−). When the isolates were inoculated at the time of sowing in pot-based systems with either sand or clay loam media, and in field trials, a significant increase in dry root and shoot biomass was recorded compared to uninoculated controls. It is likely that the strains’ plant growth promoting capacity under water stress is due to the combined effects of the bacterial phenotypes examined here.

scholarly journals Microarray Analysis of the Gene Expression Profile Induced by the Endophytic Plant Growth-Promoting Rhizobacteria, Pseudomonas fluorescens FPT9601-T5 in Arabidopsis

2005 ◽  
Vol 18 (5) ◽  
pp. 385-396 ◽  
Author(s):  
Yanqing Wang ◽  
Yumiko Ohara ◽  
Hitoshi Nakayashiki ◽  
Yukio Tosa ◽  
Shigeyuki Mayama
Keyword(s):  
Plant Growth ◽  
Pseudomonas Syringae ◽  
Microarray Analysis ◽  
Plant Growth Promoting Rhizobacteria ◽  
Plant Responses ◽  
Disease Symptoms ◽  
Global View ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Physiological And Biochemical

Pseudomonasz fluorescens FPT9601-T5 was originally identified as an endophytic plant growth-promoting rhizobacteria (PGPR) on tomato. To perform a molecular dissecttion of physiological and biochemical changes occurring in the host triggered by P. fluorescens FPT9601-T5 colonization, the model plant Arabidopsis was used in this study. Root colonization of Arabidopsis with P. fluorescens FPT9601-T5 promoted plant growth later than three weeks after inoculation and partially suppressed disease symptoms caused by Pseudomonas syringae pv. tomato DC3000, indicating that P. fluorescens FPT9601-T5 acted as a PGPR on Arabidopsis. To obtain a global view on transcript modification during the Arabidopsis-FPT9601-T5 interaction, we performed microarray analysis using Affymetrix Genechip probe arrays representing approximately 22,800 genes. The results showed that 95 and 105 genes were up- or down-regulated, respectively, more than twofold in FPT9601-T5-treated Arabidopsis plants as compared with control plants. Those up-regulated included genes involved in metabolism, signal transduction, and stress response. Noteworthy, upon FPT9601-T5 colonization, putative auxin-regulated genes and nodulin-like genes were up-regulated, and some ethylene-responsive genes were down-regulated. Our results suggest that P.fluorescens FPT9601-T5 triggered plant responses in a manner similar to known PGPR and, at least in some aspects, to rhizobia.

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