Slurry packing of high-performance columns for liquid chromatography. Part 1: Effect of quality of tube inner wall

We have assessed five high-throughput systems for the measurement of glycated haemoglobin and have reviewed published evaluations of individual analysers. All systems offered better precision than a widely used electroendosmosis method. The low pressure chromatography and immunoassay systems demonstrated greater between-batch imprecision than the high performance liquid chromatography analysers, the latter achieving the proposed analytical goal of between-batch coefficients of variation less than 5%. Agreement between all systems measuring HbA1 was good but there was variability amongst observed HbA1c values. The systems were also assessed for their quality of chromatographic separation, simplicity of operation, flexibility, cost and potential for interference by other haemoglobins.

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DEVELOPMENT AND VALIDATION OF A HIGH PERFORMANCE LIQUID CHROMATOGRAPHY (HPLC) ANALYTICAL METHOD FOR DETERMINATION OF AMOXICILLIN IN CAPSULES

Periódico Tchê Química ◽

10.52571/ptq.v13.n26.2016.78_periodico26_pgs_78_87.pdf ◽

2016 ◽

Vol 13 (26) ◽

pp. 78-87

Author(s):

Tiago Hickman IGLIN ◽

Flávia Nathiely Silveira FACHEL ◽

Amanda Gonçalves GUWZINSKI ◽

Airton Monza da SILVEIRA ◽

Filipe de Medeiros ALBANO ◽

...

Keyword(s):

Quality Control ◽

High Performance Liquid Chromatography ◽

Liquid Chromatography ◽

High Performance ◽

Forced Degradation ◽

Semisynthetic Penicillin ◽

The Stability ◽

Development And Validation

Amoxicillin, substance-related to semisynthetic penicillin, has been widely used to treat infections caused by various microorganisms, however reports of suitable methods for the quantitative determination and indicative of the stability of formulations containing this substance are rare. Due to lack of studies on the forced degradation of the substance and on the need to monitor the quality of this type of formulation was proposed and validated a method for the determination of amoxicillin content in capsules by high-performance liquid chromatography - HPLC for the quality control of amoxicillin capsules, allowing the provision of useful information about the characteristics of this type of formulation and its stability. The method was validated for parameters of linearity, specificity, accuracy, precision, and robustness

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Identification and Quantification of three Tubeimosides in Rhizoma Bolbostematis by High Performance Liquid Chromatography with Evaporative Light Scattering Detection and Electrospray Mass Spectrometric Detection

Natural Product Communications ◽

10.1177/1934578x0800300505 ◽

2008 ◽

Vol 3 (5) ◽

pp. 1934578X0800300 ◽

Cited By ~ 1

Author(s):

Hao Huang ◽

MingJin Liang ◽

Wen Xu ◽

Chuan Zhang ◽

WeiDong Zhang

Keyword(s):

High Performance Liquid Chromatography ◽

Liquid Chromatography ◽

Light Scattering ◽

High Performance ◽

Methanol Extract ◽

Gradient Elution ◽

Evaporative Light Scattering Detection ◽

Light Scattering Detection ◽

Evaporative Light Scattering

Tubeimoside I, tubeimoside II and tubeimoside III were simultaneously determined and identified by high performance liquid chromatography coupled with electrospray tandem mass spectrometry (HPLC-ESI/MS), and a novel and sensitive high performance liquid chromatography - evaporative light scattering detection (HPLC-ELSD) in a 70% methanol extract of Rhizoma Bolbostematis. The chromatographic separation was performed on a Zorbax Extend C18 analytical column using gradient elution with a solution of acetonitrile and 0.5% acetic acid. The method was validated with acceptable linearities (r > 0.9992) and recoveries (98.6 to 102.4 %). The limits of detection of these three tubeimosides were as low as 0.05 μg. The intra- and inter-day precisions of the method were evaluated and were less than 3.6%. The method was successfully used to analyze 15 batches of Rhizoma Bolbostematis. The content of tubeimosides in the plant material varied from habitat to habitat confirming the necessity to control the quality of Rhizoma Bolbostematis during its preparation and application in the clinic.

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Ocena zawartości metyloglioksalu w miodzie manuka znajdującym się na rynku krajowym

Postępy Fitoterapii ◽

10.25121/pf.2018.19.2.106 ◽

2018 ◽

Vol 19 (2) ◽

Author(s):

Deta Łuczycka ◽

Maciej Olczyk

Keyword(s):

High Performance Liquid Chromatography ◽

Liquid Chromatography ◽

High Performance ◽

Hplc Method ◽

Photometric Method ◽

Manuka Honey ◽

Promoting Effect ◽

Health Promoting ◽

Human Digestive Tract

Introduction. Recently, mass media published a number of articles doubting health promoting effect of manuka honey (MH). On the other hand, many researchers emphasize beneficial effect of honey on human digestive tract, respiratory system and skin. However, the honeys tested for their health promoting effects came from certified sources and met the applicable harvesting, storage and packing standards between the production and testing. Aim. The purpose of the study was to compare quality features of manuka honey (MH) imported to and sold in, Poland: methylglyoxal (MGO), 5-hydroxymethylfurfural (HMF) and diastase number (DN) declared for product lots by producers and distributors vs. the actual figures. Material and methods. The study tested 45 samples of MH coming from 9 producers, acquired from different local suppliers (importers and distributors), marketed in Poland in 2014-2015. The contents of MGO were measured using the high-performance liquid chromatography (HPLC) method. The contents of HMF were measured using high-performance liquid chromatography with spectrophotometric detection (HPLC-UV) in accordance with the regulation by the Polish Minister of Agriculture. DN were determined using the photometric method acc. To the regulation by the Polish Minister of Agriculture. Results. A review of the test results revealed a relationship between the declared and the actual contents of MGO. For the MH with MGO > 500, the actual values were different more frequently. For the MH with higher declared MGO contents (< 500), commercial quality of the products was worse (too high HMF and too low DN). Conclusions. The MH with MGO under 500 mg/kg can be recognized as satisfactory in terms of MGO contents and commercial quality.

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