The ultrastructural characterization of mitochondria-rich cells as a response to variations in salinity in two types of teleostean pseudobranch: milkfish (Chanos chanos) and Mozambique tilapia (Oreochromis mossambicus)

2017 ◽  
Vol 278 (3) ◽  
pp. 390-402 ◽  
Author(s):  
Sheng-Hui Yang ◽  
Jeng-Dau Tsai ◽  
Chao-Kai Kang ◽  
Wen-Kai Yang ◽  
Hsiu-Ni Kung ◽  
...  
Keyword(s):  
Oreochromis Mossambicus ◽  
Chanos Chanos ◽  
Mozambique Tilapia ◽  
Ultrastructural Characterization

Molecular characterization of sdf1 and cxcr4 in the Mozambique tilapia, Oreochromis mossambicus

2017 ◽  
Vol 176 ◽  
pp. 51-63 ◽  
Author(s):  
Jorge Amat-Fernandez ◽  
Michael J. Hammond ◽  
Di Liang ◽  
Tianfang Wang ◽  
Tomer Ventura ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Oreochromis Mossambicus ◽  
Mozambique Tilapia

scholarly journals A Chromosome-Level Genome Assembly of Mozambique Tilapia (Oreochromis mossambicus) Reveals the Structure of Sex Determining Regions

2021 ◽  
Vol 12 ◽  
Author(s):  
Wenjing Tao ◽  
Jianmeng Cao ◽  
Hesheng Xiao ◽  
Xi Zhu ◽  
Junjian Dong ◽  
...  
Keyword(s):  
Sex Determination ◽  
Genome Assembly ◽  
Oreochromis Mossambicus ◽  
Missense Mutations ◽  
Sequencing Data ◽  
High Coverage ◽  
Mozambique Tilapia ◽  
High Quality Genome ◽  
Chromosome Level

The Mozambique tilapia (Oreochromis mossambicus) is a fascinating taxon for evolutionary and ecological research. It is an important food fish and one of the most widely distributed tilapias. Because males grow faster than females, genetically male tilapia are preferred in aquaculture. However, studies of sex determination and sex control in O. mossambicus have been hindered by the limited characterization of the genome. To address this gap, we assembled a high-quality genome of O. mossambicus, using a combination of high coverage of Illumina and Nanopore reads, coupled with Hi-C and RNA-Seq data. Our genome assembly spans 1,007 Mb with a scaffold N50 of 11.38 Mb. We successfully anchored and oriented 98.6% of the genome on 22 linkage groups (LGs). Based on re-sequencing data for male and female fishes from three families, O. mossambicus segregates both an XY system on LG14 and a ZW system on LG3. The sex-patterned SNPs shared by two XY families narrowed the sex determining regions to ∼3 Mb on LG14. The shared sex-patterned SNPs included two deleterious missense mutations in ahnak and rhbdd1, indicating the possible roles of these two genes in sex determination. This annotated chromosome-level genome assembly and identification of sex determining regions represents a valuable resource to help understand the evolution of genetic sex determination in tilapias.

Ultrastructural identification of three types of sensory setae on copepod antennae

1995 ◽  
Vol 53 ◽  
pp. 956-957
Author(s):  
T. M. Weatherby ◽  
P.H. Lenz
Keyword(s):  
Phase Locking ◽  
Membrane Surface ◽  
Reaction Times ◽  
High Sensitivity ◽  
Structural Features ◽  
Calanoid Copepods ◽  
Marine Food Webs ◽  
Dendritic Membrane ◽  
Ultrastructural Characterization

Crustaceans, as well as other arthropods, are covered with sensory setae and hairs, including mechanoand chemosensory sensillae with a ciliary origin. Calanoid copepods are small planktonic crustaceans forming a major link in marine food webs. In conjunction with behavioral and physiological studies of the antennae of calanoids, we undertook the ultrastructural characterization of sensory setae on the antennae of Pleuromamma xiphias.Distal mechanoreceptive setae exhibit exceptional behavioral and physiological performance characteristics: high sensitivity (<10 nm displacements), fast reaction times (<1 msec latency) and phase locking to high frequencies (1-2 kHz). Unusual structural features of the mechanoreceptors are likely to be related to their physiological sensitivity. These features include a large number (up to 3000) of microtubules in each sensory cell dendrite, arising from or anchored to electron dense rods associated with the ciliary basal body microtubule doublets. The microtubules are arranged in a regular array, with bridges between and within rows. These bundles of microtubules extend far into each mechanoreceptive seta and terminate in a staggered fashion along the dendritic membrane, contacting a large membrane surface area and providing a large potential site of mechanotransduction.

Ultrastructural Characterization of Malpighian Tubules of Rhodnius prolixus During the Insect's Feeding Cycle and Under Hyperosmotic Shock

2020 ◽  
Vol 129 (2) ◽  
pp. 189
Author(s):  
Jacenir Reis dos Santos-Mallet ◽  
Simone Patrícia Carneiro Freitas ◽  
Maria Luiza Ribeiro de Oliveira ◽  
Alice Helena Ricardo-Silva ◽  
Aníbal Gil Lopes ◽  
...  
Keyword(s):  
Rhodnius Prolixus ◽  
Malpighian Tubules ◽  
Hyperosmotic Shock ◽  
Ultrastructural Characterization ◽  
Feeding Cycle

scholarly journals Morphological and Ultrastructural Characterization of Hemocytes in an Insect Model, the Hematophagous Dipetalogaster maxima (Hemiptera: Reduviidae)

Insects ◽  
2021 ◽  
Vol 12 (7) ◽  
pp. 640
Author(s):  
Natalia R. Moyetta ◽  
Fabián O. Ramos ◽  
Jimena Leyria ◽  
Lilián E. Canavoso ◽  
Leonardo L. Fruttero
Keyword(s):  
Cell Biology ◽  
Cell Types ◽  
Transmission Electron ◽  
Ultrastructural Characterization ◽  
Current Classification ◽  
Contrast Microscopy ◽  
First Time ◽  
Controversial Aspect

Hemocytes, the cells present in the hemolymph of insects and other invertebrates, perform several physiological functions, including innate immunity. The current classification of hemocyte types is based mostly on morphological features; however, divergences have emerged among specialists in triatomines, the insect vectors of Chagas’ disease (Hemiptera: Reduviidae). Here, we have combined technical approaches in order to characterize the hemocytes from fifth instar nymphs of the triatomine Dipetalogaster maxima. Moreover, in this work we describe, for the first time, the ultrastructural features of D. maxima hemocytes. Using phase contrast microscopy of fresh preparations, five hemocyte populations were identified and further characterized by immunofluorescence, flow cytometry and transmission electron microscopy. The plasmatocytes and the granulocytes were the most abundant cell types, although prohemocytes, adipohemocytes and oenocytes were also found. This work sheds light on a controversial aspect of triatomine cell biology and physiology setting the basis for future in-depth studies directed to address hemocyte classification using non-microscopy-based markers.

Ultrastructural characterization of a pigment mutant of the red alga Palmaria palmata

1984 ◽  
Vol 62 (6) ◽  
pp. 1101-1107 ◽  
Author(s):  
C. M. Pueschel ◽  
J. P. van der Meer
Keyword(s):  
Red Alga ◽  
Palmaria Palmata ◽  
Wild Type ◽  
Ultrastructural Examination ◽  
Dark Treatment ◽  
Prolamellar Bodies ◽  
Ultrastructural Characterization ◽  
Wild Type Control

Ultrastructural examination of a green-pigmented mutant of the red alga Palmaria palmata (L.) O. Kuntze revealed unusual features of the chloroplasts. Encircling peripheral thylakoids, characteristic of the wild-type plastids and florideophyte plastids generally, were lacking. Parallel evenly spaced thylakoids occurred in groups, leaving large volumes of thylakoid-free stroma. Irregularly shaped, electron-dense inclusions with an amorphous substructure and diameters up to 3 μm occurred in some plastids. Cells of the sporeling holdfasts contained structures resembling prolamellar bodies. Attempts to induce formation of prolamellar bodies in blades by dark treatment for 5 weeks were unsuccessful. However, some plastids did develop highly corrugated thylakoids with the crests of one thylakoid apposed to the troughs of the adjacent thylakoid. Thylakoid morphology of the wild-type control was not altered by the absence of light.

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