Cellular localization of tyrosine hydroxylase mRNA and its regulation in the rat adrenal medulla and brain by in situ hybridization with an oligodeoxyribonucleotide probe

1987 ◽  
Vol 17 (1) ◽  
pp. 11-18 ◽  
Author(s):  
V. K. M. Han ◽  
J. Snouweart ◽  
A. C. Towle ◽  
P. K. Lund ◽  
J. M. Lauder
Keyword(s):  
In Situ Hybridization ◽  
Tyrosine Hydroxylase ◽  
Adrenal Medulla ◽  
Cellular Localization

Cellular Localization of Inducible Nitric Oxide Synthase in Experimental Endotoxic Shock in the Rat

1994 ◽  
Vol 87 (2) ◽  
pp. 179-186 ◽  
Author(s):  
H. Terence Cook ◽  
Alison J. Bune ◽  
Albertine S. Jansen ◽  
G. Michael Taylor ◽  
Rashpal K. Loi ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
In Situ Hybridization ◽  
Cellular Localization ◽  
Endotoxic Shock ◽  
No Synthase ◽  
Similar Distribution ◽  
Mouse Macrophage ◽  
Inducible No Synthase ◽  
Monocytes And Macrophages

1. Endotoxin induces a shock-like syndrome with increased nitric oxide synthesis. To clarify the cellular source of NO in endotoxic shock we used immunohistochemistry and in situ hybridization to localize inducible NO synthase in rats given lipopolysaccharide or Corynebacterium parvum and lipopolysaccharide. Immunohistochemistry was carried out with an antibody raised against a synthetic peptide of mouse macrophage NO synthase. In situ hybridization was performed with 35S-labelled oligonucleotide probes corresponding to cDNA sequences common to mouse macrophage inducible NO synthase and rat vascular smooth inducible NO synthase. Monocytes and macrophages were identified by immunohistochemistry with the mouse monoclonal antibody ED1. 2. After lipopolysaccharide alone, the major site of NO synthase induction was monocytes and macrophages in multiple organs, principally liver and spleen. Bronchial, bile duct, intestinal and bladder epithelium and some hepatocytes also expressed inducible NO synthase. Expression peaked at 5 h and had returned to normal by 12 h except in spleen. 3. After priming with C. parvum, lipopolysaccharide led to a similar distribution of inducible NO synthase as lipopolysaccharide alone, but in addition there was more prominent hepatocyte staining, staining in macrophage granulomas in the liver and inducible NO synthase was present in some endothelial cells in the aorta. 4. These findings provide a direct demonstration of the cellular localization of inducible NO synthase after lipopolysaccharide.

A method for cellular localization of gene expression via quantitative in situ hybridization in plants

2007 ◽  
Vol 50 (1) ◽  
pp. 159-187 ◽  
Author(s):  
Hendrik Küpper ◽  
Laura Ort Seib ◽  
Mayandi Sivaguru ◽  
Owen A. Hoekenga ◽  
Leon V. Kochian
Keyword(s):  
Gene Expression ◽  
In Situ Hybridization ◽  
Cellular Localization

Immunocytochemical and in situ hybridization evidence for the coexistence of GABA and tyrosine hydroxylase in the rat locus ceruieus

1992 ◽  
Vol 234 (4) ◽  
pp. 593-604 ◽  
Author(s):  
Koichi Iijima ◽  
Mitsuru Sato ◽  
Naosuke Kojima ◽  
Kazuo Ohtomo
Keyword(s):  
In Situ Hybridization ◽  
Tyrosine Hydroxylase

scholarly journals Synthesis of transthyretin (pre-albumin) mRNA in choroid plexus epithelial cells, localized by in situ hybridization in rat brain.

1986 ◽  
Vol 34 (7) ◽  
pp. 949-952 ◽  
Author(s):  
A J Stauder ◽  
P W Dickson ◽  
A R Aldred ◽  
G Schreiber ◽  
F A Mendelsohn ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Epithelial Cells ◽  
Rat Brain ◽  
Choroid Plexus ◽  
Cellular Localization ◽  
Lateral Ventricles ◽  
The Brain ◽  
Albumin Mrna ◽  
Choroid Plexus Epithelial

The sites of synthesis of transthyretin in the brain were investigated using in situ hybridization with [35S]-labeled recombinant cDNA probes specific for transthyretin mRNA. Autoradiography of hybridized coronal sections of rat brain revealed specific cellular localization of transthyretin mRNA in choroid plexus epithelial cells of the lateral, third, and fourth ventricles. Transferrin mRNA was also investigated and, in contrast to transthyretin mRNA, was localized mainly in the lateral ventricles. Our results indicate that substantial synthesis of transthyretin and transferrin mRNA may occur in the choroid plexus.

scholarly journals Cellular localization of nerve growth factor synthesis by in situ hybridization.

1987 ◽  
Vol 6 (4) ◽  
pp. 891-899 ◽  
Author(s):  
C.E. Bandtlow ◽  
R. Heumann ◽  
M.E. Schwab ◽  
H. Thoenen
Keyword(s):  
In Situ Hybridization ◽  
Nerve Growth Factor ◽  
Growth Factor ◽  
Cellular Localization ◽  
Nerve Growth ◽  
Nerve Growth Factor Synthesis

Localization of tyrosine hydroxylase and its messenger RNA in the brain of rainbow trout by immunocytochemistry and in situ hybridization

2002 ◽  
Vol 449 (4) ◽  
pp. 374-389 ◽  
Author(s):  
Angelique Vetillard ◽  
Sanae Benanni ◽  
Christian Saligaut ◽  
Patrick Jego ◽  
Thierry Bailhache
Keyword(s):  
In Situ Hybridization ◽  
Rainbow Trout ◽  
Tyrosine Hydroxylase ◽  
Messenger Rna ◽  
The Brain
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