Studies on the genomic heterogeneity of Micrococcus luteus strains by macro-restriction analysis using pulsed-field gel electrophoresis

2003 ◽  
Vol 43 (4) ◽  
pp. 337-340 ◽  
Author(s):  
O. Murayama ◽  
M. Matsuda ◽  
J. E. Moore
Keyword(s):  
Gel Electrophoresis ◽  
Micrococcus Luteus ◽  
Restriction Analysis ◽  
Pulsed Field Gel Electrophoresis ◽  
Pulsed Field

Listeria monocytogenes in Five Sardinian Swine Slaughterhouses: Prevalence, Serotype, and Genotype Characterization

2013 ◽  
Vol 76 (11) ◽  
pp. 1863-1867 ◽  
Author(s):  
DOMENICO MELONI ◽  
FRANCESCA PIRAS ◽  
ANNA MUREDDU ◽  
FEDERICA FOIS ◽  
SIMONETTA GIANNA CONSOLATI ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Gel Electrophoresis ◽  
Restriction Analysis ◽  
Pulsed Field Gel Electrophoresis ◽  
Pork Meat ◽  
Pulsed Field ◽  
Restriction Profiles ◽  
Full Complement ◽  
Contact Surfaces ◽  
Slaughtered Pigs

In a 3-year study (2008 to 2011) to estimate the prevalence and the contamination sources of Listeria monocytogenes in pork meat in Sardinia, Italy, 211 samples were collected from five Sardinian swine slaughterhouses: 171 samples from slaughtered pigs and 40 from the slaughterhouse environment. Fifty L. monocytogenes isolates were characterized by PCR-based serotyping, presence of virulence-associated genes, and pulsed-field gel electrophoresis restriction analysis. The overall prevalence of L. monocytogenes was 33% in swine carcasses, 7% in cecal material, 23% on meat contact surfaces, and 25% on noncontact surfaces. Only two serotypes were detected: 1/2c (78%) and 1/2a (22%). In all, based on the presence of virulence-associated genes, eight pathogenic profiles were detected. Only 42% of all isolates carried the full complement of virulence-associated genes and were allotted to profile 1. Six pulsed-field gel electrophoresis profiles persisted in the slaughterhouses; restriction profiles appeared to be specific to each plant.

scholarly journals Evaluation of PFGE and mtDNA restriction analysis methods to detect genetic diversity of saccharomyces cerevisiae strains associated to vitis vinifera

OENO One ◽  
2007 ◽  
Vol 41 (3) ◽  
pp. 155
Author(s):  
Mar Vilanova ◽  
Sol Zamuz ◽  
Antón Masa ◽  
Carmen Sieiro
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Genetic Diversity ◽  
Mitochondrial Dna ◽  
Vitis Vinifera ◽  
Gel Electrophoresis ◽  
Restriction Analysis ◽  
Molecular Genetic ◽  
Pulsed Field Gel Electrophoresis ◽  
Pulsed Field ◽  
Mtdna Restriction Analysis

<p style="text-align: justify;"><strong>Aims</strong>: The aim of this work was realize a comparative study of two different methods of Saccharomycec cerevisiae yeast strain characterization.</p><p style="text-align: justify;"><strong>Methods and results</strong>: Pulsed-field gel electrophoresis analysis (PFGE) and mitochondrial DNA (mtDNA) restriction analysis have been carried out to differentiate strains of Saccharomyces cerevisiae associated to Vitis vinifera musts from different Galicia wineyard (NW Spain). Seventeen strains isolated from wineries from Galicia were used in this study.</p><p style="text-align: justify;"><strong>Conclusion</strong>: The results have showed that although PFGE analysis technique has greater discriminatory power than mtDNA restriction analysis to detect genetic diversity in Saccharomyces cerevisiae, some clones with the same PFGE profile can only be differentiated by mtDNA restriction analysis.</p><p style="text-align: justify;"><strong>Significance and impact of study</strong>: Pulsed-field gel electrophoresis analysis of chromosome (PFGE), by its discriminating power, constitute an ideal technique for the differentiation of Saccharomyces cerevisiae strains in biotechnological industries, however, mitochondrial DNA (mtDNA) restriction analysis is a rapid, simple and less expensive and time-consuming method. The results obtained demonstrate the value of using molecular genetic methods in taxonomic and ecological surveys.</p>

scholarly journals Use of Pulsed-Field Gel Electrophoresis to Determine the Source of Methicillin-Resistant Staphylococcus aureus Bacteremia

2021 ◽  
Vol 13 (3) ◽  
pp. 602-610
Author(s):  
Eugene Y. H. Yeung ◽  
Ivan Gorn
Keyword(s):  
Staphylococcus Aureus ◽  
Gel Electrophoresis ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Epidemiological Studies ◽  
Pelvic Trauma ◽  
Pulsed Field Gel Electrophoresis ◽  
Bacterial Strains ◽  
Methicillin Resistant ◽  
Pulsed Field ◽  
Clinical Cases

Pulsed-field gel electrophoresis (PFGE) has historically been considered the gold standard in fingerprinting bacterial strains in epidemiological studies and outbreak investigations; little is known regarding its use in individual clinical cases. The current study detailed two clinical cases in which PFGE helped to determine the source of their methicillin-resistant Staphylococcus aureus (MRSA) bacteremia. Patient A was found to have MRSA bacteremia after trauma in her pelvic area. MRSA was also found in her groin but not in her nostril and rectum. PFGE was performed that showed variable bands of her MRSA isolates from blood and groin, suggestive of different strains of MRSA. Her MRSA bacteremia was determined to be unrelated to her pelvic trauma. Patient B was found to have MRSA bacteremia after colonoscopy. MRSA was also found in his nostril and rectum. PFGE was performed that showed variable bands of his MRSA isolates from blood and rectum but identical bands of MRSA isolates from his blood and nostril. His MRSA bacteremia was determined to be unrelated to his colonoscopy procedure. The current study demonstrates the use of PFGE to rule out the source of bacteremia in individual clinical cases.

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