Changes in cytoplasmic ultrastructure during submerged cultivation of a peptide alkaloids-producing strain ofClaviceps purpurea (FR.) TUL

1982 ◽  
Vol 22 (1) ◽  
pp. 49-61 ◽  
Author(s):  
W. Lösecke ◽  
D. Neumann ◽  
H.-P. Schmauder ◽  
D. Gröger
Keyword(s):  
Submerged Cultivation

Determination of Specific Activity of Cholera Chemical Vaccine Components using Cell Culture

2020 ◽  
Vol 36 (3) ◽  
pp. 82-89
Author(s):  
O.V. Gromova ◽  
O.S. Durakova ◽  
S.V. Generalov ◽  
L.F. Livanova ◽  
O.A. Volokh
Keyword(s):  
Cell Culture ◽  
Vibrio Cholerae ◽  
Production Control ◽  
Specific Activity ◽  
Methodological Approach ◽  
Toxin Production ◽  
Submerged Cultivation ◽  
Vaccine Production ◽  
Antigenic Activity

Том 36(2020) №3 стр. 82-89; DOI 10.21519/0234-2758-2020-36-3-82-89А.В. Гаева1*, О.В. Громова1, О.С. Дуракова1, С.В. Генералов1, Л.Ф. Ливанова1, О.А. Волох1 Определение специфической активности компонентов холерной химической вакцины с использованием культуры клеток 1ФКУЗ «Российский научно-исследовательский противочумный институт «Микроб»» Федеральной службы по надзору в сфере защиты прав потребителей и благополучия человека, Саратов 410005 *[email protected] Поступила - 2019-11-26; После доработки - 2020-03-16; Принята к публикации - 2020-05-15 Список литературы Описаны методы определения динамики продукции токсинов штаммом Vibrio cholerae 569B при глубинном культивировании в биореакторе и антигенной активности специфической фракции холерогена-анатоксина по анатоксинсвязыванию с использованием клеточных культур. Показана высокая степень соответствия результатов, полученных методами, применяемыми для контроля этапов производства холерной химической вакцины и рассмотренными в данной работе. Отмечено, что применение клеточной линии СНО-К1 наиболее перспективно для замены биомоделей на промежуточных этапах контроля активных компонентов холерной химической вакцины. Разработанный методический подход впервые предлагается использовать на этапах производства холерной бивалентной химической вакцины. культура клеток, Vibrio cholerae, холерная химическая вакцина, контроль производства, холера. Vol 36(2020) N 3 p. 82-89; DOI 10.21519/0234-2758-2020-36-3-82-89A.V. Gaeva1*, O.V. Gromova1, O.S. Durakova1, S.V. Generalov1, L.F. Livanova1, O.A. Volokh1 Determination of Specific Activity of Cholera Chemical Vaccine Components using Cell Culture 1Russian Research Anti-Plague Institute «Microbe» of the Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing, Saratov, 410005 *[email protected] Received - 26.11.2019; Accepted - 15.05.2020 References The methods has been described to determine the dynamics of toxin production by the Vibrio cholerae 569B strain during submerged cultivation in bioreactor and of the antigenic activity of specific choleragen anatoxin fraction by anatoxin binding levels using cell cultures. High degree of consistency was observed between the results obtained via the method under consideration and those obtained via control methods at different stages of cholera chemical vaccine production. It was shown that the CHO-K1 cell line is the most promising substitute for biomodels at the intermediate stages of control of active cholera chemical vaccine components. The developed methodological approach was first proposed for use at the stages of cholera chemical bivalent vaccine manufacturing. cell culture, Vibrio cholerae, cholera chemical vaccine, production control, cholera.

Submerged cultivation of a strain ofHumicola lutea 72 producing acid protease

1981 ◽  
Vol 13 (3) ◽  
pp. 165-169 ◽  
Author(s):  
P. Aleksieva ◽  
A. Djerova ◽  
B. Tchorbanov ◽  
I. Grigorov
Keyword(s):  
Acid Protease ◽  
Submerged Cultivation

Pathogenicity and Lipid Composition of Mycelium of the Fungus Stagonospora cirsii VIZR 1.41 during Submerged Cultivation

2021 ◽  
Vol 57 (2) ◽  
pp. 226-235
Author(s):  
G. M. Frolova ◽  
E. R. Kotlova ◽  
S. V. Sokornova ◽  
S. V. Senik ◽  
A. L. Shavarda ◽  
...  
Keyword(s):  
Lipid Composition ◽  
Submerged Cultivation

scholarly journals Short Communication Screening of basidiomycetes in submerged cultivation based on antioxidant activity

2015 ◽  
Vol 14 (3) ◽  
pp. 9907-9914 ◽  
Author(s):  
S.H. Umeo ◽  
G.P.N. Souza ◽  
P.M. Rapachi ◽  
D.M. Garcia ◽  
L.D. Paccola-Meirelles ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Short Communication ◽  
Submerged Cultivation

scholarly journals Trametes suaveolens as ligninolytic enzyme producer

2013 ◽  
pp. 437-444 ◽  
Author(s):  
Aleksandar Knezevic ◽  
Ivan Milovanovic ◽  
Mirjana Stajic ◽  
Jelena Vukojevic
Keyword(s):  
Enzyme Activity ◽  
Solid State ◽  
Wheat Straw ◽  
Solid State Fermentation ◽  
Nitrogen Concentration ◽  
Pollution Prevention ◽  
Ligninolytic Enzyme ◽  
Species Selection ◽  
Submerged Cultivation ◽  
Phenol Red

Species of the genus Trametes represent one of the most efficient lignin-degraders which can be attributed to a well developed ligninolytic enzyme system. Current trends are screening of ability of new species to produce these enzymes, as well as the optimization of conditions for their overproduction. Therefore, the aim of the study was to evaluate the potential of T. suaveolens to synthesize laccase and Mn-oxidizing peroxidases during fermentation of the selected plant raw materials. Level of enzyme activities was measured on 7, 10 and 14th day of submersion, as well as the solid-state fermentation of wheat straw and oak sawdust in the presence of NH4NO3 in previously determined optimal nitrogen concentration of 25 mM. The enzyme activity was determined spectrophotometrically using ABTS and phenol red as the substrates. The highest level of laccase activity (1087.1 U/L) was noted after 7 days of wheat straw solid-state fermentation, while during the submerged cultivation the production of the enzyme was not noted. Submerged cultivation in oak sawdust-enriched medium was the optimal for activity of Mn-dependent peroxidase (1767.7 U/L on day 14) and Mn-independent peroxidase (1113.7 U/L on day 7). Introduction of T. suaveolens to produce ligninolytic enzyme represented the base for further study, as well as the determination of relation between enzyme activity and rate of lignin degradation. It could lead to greater possibility of fungal species selection with high delignification capacity, which could take participation in sustainable production of food, feed, fibres, and energy, environmentally friendly pollution prevention, and bioremediation.

scholarly journals Filamentous Fungus Aspergillus oryzae for Food: From Submerged Cultivation to Fungal Burgers and Their Sensory Evaluation—A Pilot Study

Foods ◽  
2021 ◽  
Vol 10 (11) ◽  
pp. 2774
Author(s):  
Neda Rousta ◽  
Coralie Hellwig ◽  
Steven Wainaina ◽  
Lukitawesa Lukitawesa ◽  
Swarnima Agnihotri ◽  
...  
Keyword(s):  
Protein Content ◽  
Aspergillus Oryzae ◽  
Submerged Fermentation ◽  
Consumer Preferences ◽  
Biomass Concentration ◽  
Downstream Processing ◽  
Food Sources ◽  
Submerged Cultivation ◽  
Minimally Processed ◽  
Oat Flour

New food sources are explored to provide food security in sustainable ways. The submerged fermentation of edible filamentous fungi is a promising strategy to provide nutritious and affordable food that is expected to have a low environmental impact. The aim of the current study was to assess the novel use of Aspergillus oryzae cultivated in submerged fermentation on oat flour as a source for food products that do not undergo secondary fermentation or significant downstream processing. The fungus was cultivated in a pilot-scale airlift bioreactor, and the biomass concentration and protein content of the biomass were assessed. A tasting with an untrained panel assessed consumer preferences regarding the taste and texture of minimally processed vegetarian and vegan burger patties made from the biomass, and how the patties fared against established meat-alternative-based patties. The cultivation of Aspergillus oryzae resulted in a yield of 6 g/L dry biomass with a protein content of 37% on a dry weight basis. The taste and texture of the minimally processed fungal burger patties were to the liking of some participants. This was also reflected in diverse feedback provided by the participants. The cultivation of the fungus on oat flour and its utilization in developing burger patties shows its promising potential for the production of nutritious food. The applications of the fungus can be further developed by exploring other favorable ways to texture and season this relatively new functional food source to the preferences of consumers.

scholarly journals Isolation and Screening of Microorganisms for the Effective Pretreatment of Lignocellulosic Agricultural Wastes

2021 ◽  
Vol 2021 ◽  
pp. 1-16
Author(s):  
Zichen Zhang ◽  
Aabid Manzoor Shah ◽  
Hassan Mohamed ◽  
Nino Tsiklauri ◽  
Yuanda Song
Keyword(s):  
Enzyme Activities ◽  
Dominant Role ◽  
Value Added ◽  
Ligninolytic Enzymes ◽  
Agricultural Wastes ◽  
Submerged Cultivation ◽  
X Ray Diffraction ◽  
Lignocellulose Conversion ◽  
Selective Delignification ◽  
Hydrolysis Of Cellulose

Lignocellulosic waste is the most abundant biorenewable biomass on earth, and its hydrolysis releases highly valued reducing sugars. However, the presence of lignin in the biopolymeric structure makes it highly resistant to solubilization thereby hindering the hydrolysis of cellulose and hemicellulose. Microorganisms are known for their potential complex enzymes that play a dominant role in lignocellulose conversion. Therefore, the current study was designed to isolate and screen potential microorganisms for their selective delignification ability for the pretreatment of lignocellulosic biomass. An extensive isolation and screening procedure yielded 36 desired isolates (22 bacteria, 7 basidiomycete fungi, and 7 filamentous fungi). Submerged cultivation of these desired microorganisms revealed 4 bacteria and 10 fungi with potent lignocellulolytic enzyme activities. The potent isolates were identified as Pleurotus, Trichoderma, Talaromyces, Bacillus, and Chryseobacterium spp. confirmed by morphological and molecular identification. The efficiency of these strains was determined through enzyme activities, and the degraded substrates were analyzed through scanning electron microscopy (SEM) and X-ray diffraction (XRD). Among all isolated microbes, Pleurotus spp. were found to have high laccase activity. The cellulose-decomposing and selective delignification strains were subjected to solid-state fermentation (SSF). SSF of field waste corn stalks as a single-carbon source provides Pleurotus spp. better condition for the secretion of ligninolytic enzymes. These isolated ligninolytic enzymes producing microorganisms may be used for the effective pretreatment of lignocellulosic agricultural wastes for the production of high value-added natural products by fermentation.

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