Cell attachment, collagen binding, and receptor analysis on bovine articular chondrocytes

2000 ◽  
Vol 18 (3) ◽  
pp. 364-373 ◽  
Author(s):  
David L. Reid ◽  
Margaret B. Aydelotte ◽  
Jurgen Mollenhauer
Keyword(s):  
Articular Chondrocytes ◽  
Cell Attachment ◽  
Collagen Binding ◽  
Bovine Articular Chondrocytes

BEHAVIOR OF ARTICULAR CHONDROCYTES ON NANOENGINEERED SURFACES

Nano LIFE ◽  
2013 ◽  
Vol 03 (03) ◽  
pp. 1342001
Author(s):  
JAMEEL SHAIK ◽  
JAVEED SHAIKH MOHAMMED ◽  
MICHAEL J. MCSHANE ◽  
DAVID K. MILLS
Keyword(s):  
Articular Chondrocytes ◽  
Cell Attachment ◽  
Cellular Growth ◽  
Tissue Formation ◽  
Initial Cell ◽  
Bovine Articular Chondrocytes ◽  
Poly Ethylene ◽  
Poly Styrene Sulfonate ◽  
Imagej Software ◽  
And Behavior

In tissue engineering, surface modification has becomes one of the leading methods to enhance initial cell attachment and subsequent cellular growth, differentiation and tissue formation. This work studied growth and behavior of primary bovine articular chondrocytes on self-assembled multilayer nanofilms composed of: polyelectrolytes [poly(styrene sulfonate) (PSS), poly-L-lysine (PLL), poly-D-lysine (PDL), chondroitin sulfate (CS), poly(ethyleneimine) (PEI), poly(dimethyldiallylammonium chloride) (PDDA), poly(ethylene glycol) amine (PEG - NH 2)] and proteins [bovine serum albumin (BSA), collagen, fibronectin, laminin]. These biomaterials were used to build mono-, bi-, and tri-layer nanofilm architectures. Potential cytotoxic effects were assessed using Live/Dead assay and cell proliferation was quantified using MTT assay. Bright field and fluorescence microscopy were used to analyze chondrocyte morphology. ImageJ software was used to analyze the number, mean area, circularity and Feret's diameter of viable cells. Cumulative results demonstrated that chondrocyte growth; proliferation and functionality were dependent on initial cell density, nanofilm thickness and material composition of nanofilms.

scholarly journals Histamine H2 receptors on foetal-bovine articular chondrocytes

1983 ◽  
Vol 212 (2) ◽  
pp. 517-520 ◽  
Author(s):  
D J Taylor ◽  
J R Yoffe ◽  
D E Woolley
Keyword(s):  
Cyclic Amp ◽  
Adenylate Cyclase ◽  
Dose Response ◽  
Response Curve ◽  
Articular Chondrocytes ◽  
Monolayer Cultures ◽  
H2 Receptors ◽  
Bovine Articular Chondrocytes ◽  
The Right ◽  
H1 Antagonists

The dose-response curve of histamine-induced cyclic AMP elevation in monolayer cultures of primary foetal-bovine articular chondrocytes was displaced to the right by cimetidine. In addition, H2 but not H1 antagonists prevented the histamine-induced cyclic AMP elevation, suggesting histamine activates chondrocyte adenylate cyclase through an H2 receptor.

Internalization of hyaluronan by chondrocytes occurs via receptor-mediated endocytosis

1993 ◽  
Vol 106 (1) ◽  
pp. 365-375 ◽  
Author(s):  
Q. Hua ◽  
C.B. Knudson ◽  
W. Knudson
Keyword(s):  
Cell Surface ◽  
Articular Chondrocytes ◽  
Dark Field ◽  
Cartilage Tissue ◽  
Receptor Mediated Endocytosis ◽  
Dark Field Microscopy ◽  
Bovine Articular Chondrocytes ◽  
Hyaluronan Receptors ◽  
Hyaluronan Receptor

Several studies have suggested that chondrocytes must have the capacity to internalize and degrade extracellular hyaluronan. In the present study we show direct evidence that hyaluronan is, in fact, endocytosed by chondrocytes and that the endocytosis is mediated via cell surface CD44/hyaluronan receptors. Cultures of bovine articular chondrocytes as well as rat chondrosarcoma chondrocytes were incubated with either fluorescein- or 3H-labeled hyaluronan. Intense binding and accumulation of labeled hyaluronan was visualized by fluorescence microscopy or bright-field/dark-field microscopy following autoradiography. Cell surface hyaluronan was removed with either trypsin or Streptomyces hyaluronidase in order to distinguish and quantify intracellular endocytosed hyaluronan. Labeled hyaluronan was visualized within small discrete intracellular vesicles distributed throughout the cytoplasm. Binding and endocytosis of fluorescein- or 3H-labeled hyaluronan was totally blocked by the addition of excess unlabeled hyaluronan or hyaluronan hexasaccharides, competitive inhibitors of hyaluronan/hyaluronan receptor interactions. Binding and endocytosis was also blocked by the addition of anti-CD44 monoclonal antibodies. Characterization of endocytosed 3H-labeled hyaluronan demonstrated that a significant portion of the hyaluronan was degraded by both the bovine articular and rat chondrosarcoma chondrocytes. Interestingly, a higher proportion of bound hyaluronan was internalized by the bovine chondrocytes. Therefore, hyaluronan receptor-mediated endocytosis and degradation of hyaluronan may provide a critical link to the maintenance and homeostasis of cartilage tissue.

scholarly journals Enhanced chondrogenic phenotype of primary bovine articular chondrocytes in Fibrin-Hyaluronan hydrogel by multi-axial mechanical loading and FGF18

2020 ◽  
Vol 105 ◽  
pp. 170-179 ◽  
Author(s):  
Bernardo P. Antunes ◽  
M. Letizia Vainieri ◽  
Mauro Alini ◽  
Efrat Monsonego-Ornan ◽  
Sibylle Grad ◽  
...  
Keyword(s):  
Mechanical Loading ◽  
Articular Chondrocytes ◽  
Chondrogenic Phenotype ◽  
Bovine Articular Chondrocytes

Nutrient Utilization by Bovine Articular Chondrocytes: A Combined Experimental and Theoretical Approach

2005 ◽  
Vol 127 (5) ◽  
pp. 758-766 ◽  
Author(s):  
Bram G. Sengers ◽  
Hannah K. Heywood ◽  
David A. Lee ◽  
Cees W. J. Oomens ◽  
Dan L. Bader
Keyword(s):  
Oxygen Uptake ◽  
High Glucose ◽  
Articular Chondrocytes ◽  
Lactate Production ◽  
Glucose Medium ◽  
Numerical Predictions ◽  
High Glucose Medium ◽  
Biosensor System ◽  
Wide Range ◽  
Bovine Articular Chondrocytes

A combined experimental-numerical approach was adopted to characterize glucose and oxygen uptake and lactate production by bovine articular chondrocytes in a model system. For a wide range of cell concentrations, cells in agarose were supplemented with either low or high glucose medium. During an initial culture phase of 48h, oxygen was monitored noninvasively using a biosensor system. Glucose and lactate were determined by medium sampling. In order to quantify glucose and oxygen uptake, a finite element approach was adopted to describe diffusion and uptake in the experimental model. Numerical predictions of lactate, based on simple relations for cell metabolism, were found to agree well for low glucose, but not for high glucose medium. Oxygen did not play a role in either case. Given the close association between chondrocyte energy metabolism and matrix synthesis, a quantifiable prediction of utilization can present a valuable contribution in the optimization of tissue engineering conditions.

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