scholarly journals Growth factors produced by bone marrow stromal cells on nanoroughened titanium–aluminum–vanadium surfaces program distal MSCs into osteoblasts via BMP2 signaling

2020 ◽  
Author(s):  
Michael B. Berger ◽  
Kyla B. Bosh ◽  
Thomas W. Jacobs ◽  
D. Joshua Cohen ◽  
Zvi Schwartz ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Growth Factors ◽  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
Marrow Stromal Cells ◽  
Titanium Aluminum ◽  
Titanium Aluminum Vanadium

Regulation of insulin-like growth factors I and II and their binding proteins in human bone marrow stromal cells by dexamethasone

1998 ◽  
Vol 71 (3) ◽  
pp. 449-458 ◽  
Author(s):  
Su-Li Cheng ◽  
Shu-Fang Zhang ◽  
Subburaman Mohan ◽  
Fernando Lecanda ◽  
Aurora Fausto ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Growth Factors ◽  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
Binding Proteins ◽  
Marrow Stromal Cells ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Insulin Like Growth Factors

Rat tail suspension reduces messenger RNA level for growth factors and osteopontin and decreases the osteoblastic differentiation of bone marrow stromal cells

2009 ◽  
Vol 10 (3) ◽  
pp. 415-423 ◽  
Author(s):  
Renwen Zhang ◽  
Scott C. Supowit ◽  
Gordon L. Klein ◽  
Zhaofeng Lu ◽  
Monique D. Christensen ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Growth Factors ◽  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
Messenger Rna ◽  
Osteoblastic Differentiation ◽  
Marrow Stromal Cells ◽  
Tail Suspension ◽  
Rat Tail

Fibroblast growth factors 1 and 2 inhibit adipogenesis of human bone marrow stromal cells in 3D collagen gels

2015 ◽  
Vol 338 (2) ◽  
pp. 136-148 ◽  
Author(s):  
Solange Le Blanc ◽  
Meike Simann ◽  
Franz Jakob ◽  
Norbert Schütze ◽  
Tatjana Schilling
Keyword(s):  
Bone Marrow ◽  
Growth Factors ◽  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
Fibroblast Growth Factors ◽  
Marrow Stromal Cells ◽  
Human Bone ◽  
Fibroblast Growth ◽  
Collagen Gels ◽  
3D Collagen

scholarly journals Monocytes stimulate fibroblastoid bone marrow stromal cells to produce multilineage hematopoietic growth factors

Blood ◽  
1986 ◽  
Vol 68 (2) ◽  
pp. 530-534 ◽  
Author(s):  
VC Broudy ◽  
KS Zuckerman ◽  
S Jetmalani ◽  
JH Fitchen ◽  
GC Jr Bagby
Keyword(s):  
Bone Marrow ◽  
Growth Factors ◽  
Conditioned Medium ◽  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
Mononuclear Phagocytes ◽  
Marrow Stromal Cells ◽  
Conditioned Media ◽  
Hematopoietic Growth Factors ◽  
Colony Stimulating Activity

Abstract In previous studies we have found that monocytes produce soluble factors that stimulate human umbilical vein endothelial cells to produce granulocyte-macrophage colony-stimulating activity (CSA), burst- promoting activity (BPA), and megakaryocyte colony-stimulating activity (Meg-CSA) as well as factors that stimulate T lymphocytes and neonatal fibroblasts to produce CSA. To test the hypothesis that monocytes would similarly stimulate the production of hematopoietic growth factors by autologous bone marrow stromal cells, multiply-passaged adherent fibroblastoid cells derived from the bone marrow of normal volunteers were exposed to conditioned media prepared by incubating autologous peripheral blood monocytes in complete medium for three days. When conditioned media from stromal cells incubated in monocyte-conditioned medium were compared with those of stromal cells cultured in the absence of monocyte-conditioned medium, BPA was increased fourfold and CSA was increased more than 30-fold. We conclude that mononuclear phagocytes recruit stromal cells of the marrow to produce multilineage growth factors in vitro. We suggest that these monocyte-derived recruiting activities may play an important role in orchestration of hematopoietic growth factor production by cells of the marrow microenvironment.

scholarly journals Graphene-Modified Titanium Surface Enhances Local Growth Factor Adsorption and Promotes Osteogenic Differentiation of Bone Marrow Stromal Cells

2021 ◽  
Vol 8 ◽  
Author(s):  
Jiayu Lu ◽  
Jiayue Sun ◽  
Derong Zou ◽  
Jinlin Song ◽  
Sheng Yang
Keyword(s):  
Bone Marrow ◽  
Growth Factors ◽  
Osteogenic Differentiation ◽  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
Electrostatic Interactions ◽  
Early Stage ◽  
Pure Titanium ◽  
Marrow Stromal Cells ◽  
Concentrated Growth Factors

Graphene coating exhibits excellent abilities of protein adsorption and cell adhesion, which might expand the osteogenic activity of titanium implant surface to adapt to the environment of low bone mass and poor bone quality. In this paper, we designed and explored the graphene-coated titanium sheet, through the surface modification of oxygen-containing functional groups, to optimize the adsorption capacity of material by improving the electrostatic interactions, and successfully adsorbed and sustained-released a variety of osteogenic related growth factors in the autologous concentrated growth factors. Compared with the pure titanium, we observed that the bone marrow stromal cells (BMSCs) on the graphene-coated titanium with concentrated growth factors showed a flat shape and expressed osteogenic related genes and proteins, while the coating surfaces promoted and accelerated the osteogenic differentiation ability of BMSCs. The results suggested that it might be a feasible alternative to improve the osteogenesis of dental implant in the early stage.

scholarly journals Regulation of cytokine expression by interferon-alpha in human bone marrow stromal cells: inhibition of hematopoietic growth factors and induction of interleukin-1 receptor antagonist

Blood ◽  
1994 ◽  
Vol 84 (12) ◽  
pp. 4142-4150 ◽  
Author(s):  
MJ Aman ◽  
U Keller ◽  
G Derigs ◽  
M Mohamadzadeh ◽  
C Huber ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Growth Factors ◽  
Receptor Antagonist ◽  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
Interleukin 1 ◽  
Marrow Stromal Cells ◽  
Hematopoietic Growth Factors ◽  
Gm Csf ◽  
Ifn Alpha

We investigated the effects of interferon-alpha (IFN-alpha) on the expression of cytokines by human bone marrow stromal cells. Production of granulocyte-macrophage colony-stimulating factor (GM-CSF), granulocyte-CSF (G-CSF), and interleukin-1 beta (IL-1 beta) in stromal cell layers was induced by incubation with IL-1 alpha, tumor necrosis factor (TNF), or lipopolysaccharide (LPS). Addition of IFN-alpha to such stimulated cultures resulted in a strong downregulation of mRNA expression of GM-CSF and IL-1 beta. Similarly, the protein levels of GM- CSF and IL-1 beta were significantly reduced by IFN-alpha, whereas G- CSF production was only moderately inhibited. In contrast, IFN-alpha markedly stimulated the production of IL-1 receptor antagonist (IL-1RA) by stromal cells. The inhibition of cytokine expression resulted in a reduced hematopoietic activity of stromal cells, indicated by a reduced proliferation of the factor dependent cell line MO7e on IFN-alpha- treated stromal cells. In the presence of cycloheximide (CHX), IFN- alpha failed to inhibit IL-1 mRNA expression, whereas the regulation of GM-CSF and IL-1RA by IFN-alpha was not affected. Our results indicate that the myelosuppressive effects of IFN-alpha, as observed in therapeutic applications or associated with viral infections, are, in part, indirectly mediated by inhibition of the paracrine production of hematopoietic growth factors.

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