Air-Liquid Interface (ALI) Culture of Human Bronchial Epithelial Cell Monolayers as an in vitro Model for Airway Drug Transport Studies

2007 ◽  
Vol 96 (2) ◽  
pp. 341-350 ◽  
Author(s):  
Hongxia Lin ◽  
Hong Li ◽  
Hyun-Jong Cho ◽  
Shengjie Bian ◽  
Hwan-Jung Roh ◽  
...  
Keyword(s):  
Drug Transport ◽  
In Vitro Model ◽  
Bronchial Epithelial Cell ◽  
Bronchial Epithelial ◽  
Cell Monolayers ◽  
Transport Studies ◽  
Epithelial Cell Monolayers ◽  
Vitro Model ◽  
Air Liquid Interface

In vitro model of “wound healing” analyzed by laser scanning cytometry: Accelerated healing of epithelial cell monolayers in the presence of hyaluronate

Cytometry ◽  
2003 ◽  
Vol 53A (1) ◽  
pp. 1-8 ◽  
Author(s):  
Asifa S. Haider ◽  
Jerzy Grabarek ◽  
Ben Eng ◽  
Paulina Pedraza ◽  
Nicholas R. Ferreri ◽  
...  
Keyword(s):  
Wound Healing ◽  
Epithelial Cell ◽  
Laser Scanning ◽  
In Vitro Model ◽  
Cell Monolayers ◽  
Laser Scanning Cytometry ◽  
Epithelial Cell Monolayers ◽  
Vitro Model

An in vitro Model of Pseudomonas aeruginosa Biofilms on Viable Airway Epithelial Cell Monolayers

2008 ◽  
Vol 22 (3) ◽  
pp. 235-238 ◽  
Author(s):  
Bradford A. Woodworth ◽  
Edwin Tamashiro ◽  
Geeta Bhargave ◽  
Noam A. Cohen ◽  
James N. Palmer
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Epithelial Cell ◽  
In Vitro Model ◽  
Airway Epithelial Cell ◽  
Airway Epithelial ◽  
Cell Monolayers ◽  
Epithelial Cell Monolayers ◽  
Vitro Model

The Influence and Mechanism of Paeoniflorin and Albiflorin on Strychnine and Brucine Transport Through Madin-Darby Canine Kidney/Multidrug Resistance1 Cells In Vitro Model

2020 ◽  
Vol 14 (5) ◽  
pp. 616-623
Author(s):  
Yun-Feng Liu ◽  
Yong-Mei Guan ◽  
Shi-Yu Huang ◽  
Lu Wu ◽  
Wei-Feng Zhu ◽  
...  
Keyword(s):  
In Vitro Model ◽  
Efflux Rate ◽  
Madin Darby Canine Kidney ◽  
Expression Levels ◽  
Transport Studies ◽  
Mdr1 Mrna ◽  
Vitro Model ◽  
Darby Canine Kidney ◽  
Canine Kidney

This research sought to study the influence and potentialmechanism of paeoniflorin and albiflorin on strychnine and brucine transport in MDCK-MDR1 cells regulated by P-gp. Cytotoxicity of drugs was tested by MTT assay, and the transport studies were performed in both directions in MDCK-MDR1 cells. The influence of drugs on P-gp ATPase, and the efflux function of P-gp, the expression levels of P-gp and MDR1 mRNA were also estimated. Strychnine and brucine showed well absorption, and the main transport mechanism might be passive diffusion. Verapamil could significantly decrease the efflux rate (ER) of strychnine and brucine, while the ER of strychnine and brucine was increased significantly when co-administrated with paeoniflorin or albiflorin, indicating that paeoniflorin and albiflorin could promote the efflux of these two alkaloids. Strychnine and brucine could activate the activity of P-gp ATPase, suppress the efflux function of P-gp, but have no significant effect on the expression of P-gp. In addition, strychnine could upregulate the expression of MDR1 mRNA. Paeoniflorin and albiflorin could increase the transmembrane transport of strychnine and brucine mediated by P-gp when co-administrated with strychnine or brucine via stimulating the activity of P-gp ATPase, enhancing the efflux function of P-g, increasing the expression levels of MDR1 mRNA and P-gp.

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