Physicochemical characterization and linear epitopes identification of arginine kinase allergen from Crassostrea gigas

Costa Rica muestra una condición incipiente en el desarrollo de la acuicultura marina, a pesar de los esfuerzos invertidos por más de veinte años. La experiencia en el cultivo de Crassostrea gigas alcanzada por comunidades, la institucionalidad pública y ONG´s, desde el ámbito social- económico y ambiental, evidencian que el país cuenta con las condiciones idóneas, para direccionar la ostricultura como un nuevo sector socio-económico con y desde las comunidades marino costeras del litoral pacífico. El presente trabajo expone los logros obtenidos por comunidades y actores locales; además los retos que ambas partes se han propuesto para hacer de esta actividad una herramienta más que contribuye con la sustentabilidad y empoderamiento de familias y comunidades que actualmente se encuentran en condiciones de pobreza.

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Characterization of Major Allergens of Local Mud Crab (Scylla serrata)

Scientific Research Journal ◽

10.24191/srj.v12i1.5434 ◽

2015 ◽

Vol 12 (1) ◽

pp. 1 ◽

Cited By ~ 1

Author(s):

Rosmilah Misnan ◽

Nurul Izzah Abdul Rahman ◽

Zailatul Hani Mohd Yadzir ◽

Noormalin Abdullah ◽

Mohd Faizal Bakhtiar ◽

...

Keyword(s):

Mass Spectrometry ◽

Arginine Kinase ◽

Mass Spectrometry Analysis ◽

Scylla Serrata ◽

Mud Crab ◽

Meat Allergy ◽

Proteomic Approach ◽

The World ◽

Major Allergens ◽

Crab Meat

Crab meat is widely consumed in several countries around the world. However, when consumed, crab meats are frequent cause of allergic reactions throughout the world. Scylla serrata is among the most common mud crab in Malaysia. In a previous study two major allergens of mud crab at 36 and 41 kDa was identified. Thus, the aim of this study is to further identify these major allergens by a proteomic approach. Protein extract was prepared and resolved by 2-dimensional electrophoresis (2-DE). Immunoblotting was then performed using reactive sera from patients with crab allergy. Major allergenic spots were then excised from the 2-DE gel and analysed by mass spectrometry. The 2-DE profile of the extract revealed approximately >100 protein spots between pH of 4.00 to 8.00. Mass spectrometry analysis has identified the 36 and 41 kDa proteins as tropomyosin and arginine kinase, respectively. Our findings indicated that tropomyosin and arginine kinase play a major role in allergic reaction to mud crab meat among local patients with crab meat allergy, and should be included in diagnostics and therapeutic strategies of this allergy.

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Solid Dispersions: A Review

International Journal of Drug Delivery Technology ◽

10.25258/ijddt.v4i2.8857 ◽

2014 ◽

Vol 4 (2) ◽

Author(s):

Sumant Saini ◽

Yashwant .

Keyword(s):

Solid Dispersions ◽

Physicochemical Characterization ◽

Water Soluble ◽

Absolute Minimum ◽

Technology Transfers ◽

Molecular Arrangement ◽

Water Soluble Drugs ◽

Carrier Solvent ◽

Preparation Techniques ◽

Selection Of

Solid dispersions are one of the most promising strategies to improve the oral bioavailability of poorly water soluble drugs. By reducing drug particle size to the absolute minimum, and hence improving drug wettability, bioavailability may be significantly improved. This article reviews the various preparation techniques for solid dispersion and compiles some of the recent technology transfers. The different types of solid dispersions based on the molecular arrangement have been highlighted. Some of the practical aspects to be considered for the preparation of solid dispersions, such as selection of carrier, solvent and methods of physicochemical characterization, along with an insight into the molecular arrangement of drugs in solid dispersions are also discussed. In this review, it is intended to discuss the recent advances related on the area of solid dispersions.

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