Optimization extraction and purification of biological activity curcumin from Curcuma longa L by high‐performance counter‐current chromatography

2020 ◽  
Vol 43 (8) ◽  
pp. 1586-1592 ◽  
Author(s):  
Yan Pan ◽  
Ronghui Ju ◽  
Xueli Cao ◽  
Hairun Pei ◽  
Tianhao Zheng ◽  
...  
Keyword(s):  
Biological Activity ◽  
High Performance ◽  
Curcuma Longa ◽  
Performance Counter ◽  
Extraction And Purification ◽  
Counter Current

scholarly journals Development of an Efficient Protocol for Cimifugin Isolation from Peucedanum schottii and Evaluation of Enzyme Inhibitory Activity

2016 ◽  
Vol 11 (8) ◽  
pp. 1934578X1601100
Author(s):  
Ewelina Kozioł ◽  
Ilkay Erdogan Orhan ◽  
F. Sezer Senol ◽  
Kalina Alipieva ◽  
Milen Georgiev ◽  
...  
Keyword(s):  
Nmr Spectroscopy ◽  
Ethyl Acetate ◽  
Crude Extract ◽  
Inhibitory Activity ◽  
High Performance ◽  
Performance Counter ◽  
Inhibitory Potential ◽  
Counter Current ◽  
First Time ◽  
Analytical Scale

The dichloromethane (DCM) extract of the fruits of Peucedanum schottii Besser ex DC. (Apiaceae) was subjected to high-performance counter-current chromatography (HPCCC) for the efficient and fast separation (30 min) and isolation of cimifugin using an ethyl acetate: water (1:1 v/v, K = 1.01) system. The analytical scale-optimized separation was easily scaled to semi-preparative conditions. Cimifugin (11.25% yield, 96.5% purity) was isolated for the first time from P. schottii and characterized by NMR spectroscopy. Cimifugin and the crude DCM extract were evaluated using ELISA microtiter assays for their inhibitory potential against the cholinesterases (acetylcholinesterase - AChE and butyrylcholinesterase - BChE), and tyrosinase (TYR), which are key enzymes for the treatment of some neurodegenerative diseases, i.e. Alzheimer's and Parkinson's. The crude extract exhibited a weak inhibitory activity against AChE, BChE, and TYR (4.2, 35.5, and 0% at 100 μg mL−1 and 10.3, 40.0, and 12.2% at 200 μg mL−1, respectively), while cimifugin displayed low to moderate inhibition towards AChE and BChE (3.1 and 21.6%, respectively) at 200 μg mL−1.

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