Characterization of a novel standardized human three‐dimensional skin wound healing model using non‐sequential fractional ultrapulsed CO 2 laser treatments

2015 ◽  
Vol 47 (3) ◽  
pp. 257-265 ◽  
Author(s):  
Yvonne Marquardt ◽  
Philipp M. Amann ◽  
Ruth Heise ◽  
Katharina Czaja ◽  
Timm Steiner ◽  
...  
Keyword(s):  
Wound Healing ◽  
Three Dimensional ◽  
Skin Wound ◽  
Skin Wound Healing ◽  
Laser Treatments ◽  
Wound Healing Model ◽  
Healing Model

Interleukin-1 Beta Regulates Lysophosphatidic Acid-accelerated Skin Wound Healing

2020 ◽  
Author(s):  
Delong Ge ◽  
Xiaoxiao Mou ◽  
Liying Liu ◽  
Yanwei Li ◽  
Hui Jiang ◽  
...  
Keyword(s):  
Wound Healing ◽  
Interleukin 1 ◽  
Skin Wound ◽  
Inflammatory Factor ◽  
Interleukin 1 Beta ◽  
Skin Wound Healing ◽  
Wound Healing Model ◽  
Kinetics Of ◽  
Healing Model

Abstract Background: Interleukin 1 beta (IL-1β) is considered to be a mediator of infectious, inflammatory and autoimmune diseases, and the kinetics of its production is relevant to understanding the pathogenesis of these diseases. Lysophosphatidic acid (LPA), the structurally simplest bioactive phospholipid, is necessary for homeostasis in various physiological and pathophysiological processes and plays a pivotal role in wound healing. Skin trauma can not only weaken the barrier function, but also cause pain and infection. Chronic wounds are characterized by impaired healing and uncontrolled inflammation that damages the protection of the immune system. The aim of this study is to investigate whether inflammatory factor IL-1β has an effect on LPA in the wound healing model. Results: In this study, the kinetics of IL-1β gene expression was studied in vivo and in vitro with a wound healing model by quantitative real-time polymerase chain reaction (qRT-PCR) through LPA treatment. As a result, we found that LPA up-regulated inflammatory factor IL-1β in HaCaT cell and skin wound healing. The pro-inflammatory cytokines IL-1β mRNA had higher expression in LPA-treated mice group 3 days after the treatment. In vitro, after the treatment with LPA (20 μM) for 6, 12, and 24 hours, IL-1β mRNA expression increased by 61.16%, 129.39%, and 117.07%, respectively. Conclusion: These results strongly suggest that IL-1β may regulate LPA-accelerated skin wound healing. IL-1β has significant efficacy, and our observations are of interest to the development of drugs targeting LPA in skin therapy.

Skin Wound Healing Model - Excisional Wounding and Assessment of Lesion Area

BIO-PROTOCOL ◽  
2015 ◽  
Vol 5 (22) ◽  
Author(s):  
Camila Moreira ◽  
Puebla Cassini-Vieira ◽  
Mariana da Silva ◽  
Luc�ola da Barcelos
Keyword(s):  
Wound Healing ◽  
Skin Wound ◽  
Lesion Area ◽  
Skin Wound Healing ◽  
Wound Healing Model ◽  
Healing Model

scholarly journals Xanthohumol-supplemented beer modulates angiogenesis and inflammation in a skin wound healing model. Involvement of local adipocytes

2011 ◽  
Vol 113 (1) ◽  
pp. 100-109 ◽  
Author(s):  
Rita Negrão ◽  
Raquel Costa ◽  
Delfim Duarte ◽  
Tiago Taveira Gomes ◽  
Pedro Coelho ◽  
...  
Keyword(s):  
Wound Healing ◽  
Skin Wound ◽  
Skin Wound Healing ◽  
Wound Healing Model ◽  
Healing Model

A Tissue-Engineered Corneal Wound Healing Model for the Characterization of Reepithelialization

2013 ◽  
pp. 59-78 ◽  
Author(s):  
Karine Zaniolo ◽  
Patrick Carrier ◽  
Sylvain L. Guérin ◽  
François A. Auger ◽  
Lucie Germain
Keyword(s):  
Wound Healing ◽  
Corneal Wound Healing ◽  
Corneal Wound ◽  
Wound Healing Model ◽  
Healing Model

scholarly journals Enzyme-Crosslinked Gelatin Hydrogel with Adipose-Derived Stem Cell Spheroid Facilitating Wound Repair in the Murine Burn Model

Polymers ◽  
2020 ◽  
Vol 12 (12) ◽  
pp. 2997
Author(s):  
Ting-Yu Lu ◽  
Kai-Fu Yu ◽  
Shuo-Hsiu Kuo ◽  
Nai-Chen Cheng ◽  
Er-Yuan Chuang ◽  
...  
Keyword(s):  
Wound Healing ◽  
Wound Repair ◽  
Three Dimensional ◽  
Stromal Vascular Fraction ◽  
Skin Wound ◽  
3D Scaffold ◽  
Skin Wound Healing ◽  
Cell Spheroid ◽  
Cell Spheroids ◽  
Adipose Derived Stem Cell

Engineered skin that can facilitate tissue repair has been a great advance in the field of wound healing. A well-designed dressing material together with active biological cues such as cells or growth factors can overcome the limitation of using auto-grafts from patients. Recently, many studies showed that human adipose-derived stem cells (hASCs) can be used to promote wound healing and skin tissue engineering. hASCs have already been widely applied for clinical trials. hASCs can be harvested abundantly because they can be easily isolated from fat tissue known as the stromal vascular fraction (SVF). On the other hand, increasing studies have proven that cells from spheroids can better simulate the biological microenvironment and can enhance the expression of stemness markers. However, a three-dimensional (3D) scaffold that can harbor implanted cells and can serve as a skin-repaired substitute still suffers from deficiency. In this study, we applied a gelatin/microbial transglutaminase (mTG) hydrogel to encapsulate hASC spheroids to evaluate the performance of 3D cells on skin wound healing. The results showed that the hydrogel is not toxic to the wound and that cell spheroids have significantly improved wound healing compared to cell suspension encapsulated in the hydrogel. Additionally, a hydrogel with cell spheroids was much more effective than other groups in angiogenesis since the cell spheroid has the possibility of cell–cell signaling to promote vascular generation.

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